Sains Ma1aysiana 25(1): 87-97 (1996)                                                                                          Sains Hayat/

Life Sciences

A Combined SEM and TEM Studies of

Microsporum canis After in Vitro Treatment with

Atratoxin B₁ from Holothuria atra

Darah, I., Teo, M. L. & Ibrahim C. O.

School of Biological Sciences

Universiti Sains Malaysia

11800 Minden, Pulau Pinang Malaysia

ABSTRACT

Atratoxin B₁ which was extracted from a local sea cucumber, Holothuria atra is a potential antifungal agent against dermatophytes. Exposure of Microsporum canis mycelia inoculated into Sabouraud glucose agar medium to the 10 mg/ml atratoxin B₁ solution, resulted in complete suppression of the fungal growth. Prolonged exposure to the atratoxin B_] (72 hours) resulted with necrosis in a substantial portion of the existing hyphae. The main anomalies noted were the abnormally shaped hyphae and the alterations in its morphology and cytology.

ABSTRAK

Atratoksin B_] yang diekstrak daripada timun laut tempatan, Holothuria atra, adalah suatu agen antikulat yang berpotensi terhadap dermatofit. Pendedahan miselium Microsporum canis yang diinokulat ke dalam medium agar glukosa Sabouraud kepada larutan atratoksin B_] berkepekatan 10 mg/ml, mengakibatkan penindasan lengkap dalam pertumbuhannya. Pendedahan yang lebih lama kepada larutan atratoksin B_] (72 jam) mengakibatkan nekrosis berlaku pada sebahagian besar hifanya. Keganjilan yang jelas berlaku adalah ketidaknormalan pada bentuk hifanya dan juga pengubahsuaian yang terjadi pada morfologi dan sitologinya.

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