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Sains Malaysiana 43(5)(2014): 739–744
Phylogenetic Analysis of Respiratory Syncytial Virus Identified at Universiti Kebangsaan Malaysia Medical Centre (Analisis Filogenetik Virus
Pernafasan Sinsitium di Pusat Perubatan, Universiti Kebangsaan Malaysia)
M.M. RAHMAN*1, K.K.
WONG1, I.
ISAHAK2, Z.Z.
RASHID1& H. ALFIZAH1 1Department of Microbiology & Immunology,
Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre,
Jalan Yaacob Latiff, Cheras, 56000 Kuala Lumpur, Malaysia
2Department of Medical Sciences, Faculty
of Medicine and Health Sciences Universiti Sains Islam Malaysia (USIM), Tingkat
13, Menara B, Persiaran MPAJ
Jalan Pandan Utama, 55100 Kuala Lumpur, Malaysia
Received: 22 May 2012/Accepted: 1
September 2013
ABSTRACT
Human respiratory syncytial virus (RSV)
is an important cause of acute respiratory tract infection in infants and young
children. Phylogenetic analysis for RSV in Malaysia has not been
reported before. We investigated the genetic features of RSV in
respiratory specimens from March to August 2011 with molecular methods. From a
total of 130 throat swab and nasopharyngeal aspirate specimens, 54 (41.5%) were
positive with RSV, identified by in-house real-time reverse
transcriptase polymerase chain reaction (rRT-PCR)
assay. Thirty-four out of 54 (63.0%) RSV positive patients were
children below two years old and two (1.4%) were adults. Phylogenetic analysis
showed 39 isolates were genotype GA5, 13 genotypes GA2,
one genotype GA1 and one genotype GA7.
The findings indicated four genotypes of RSV circulating in the country
and the predominant genotype is GA5.
Keywords: Genotype GA1, GA2, GA5 and GA7; phylogenetic analysis;
respiratory syncytial virus
ABSTRAK
Virus pernafasan sinsitium (RSV)
adalah punca utama yang menyebabkan jangkitan saluran pernafasan akut pada bayi
dan kanak-kanak. Analisis filogenetik untuk RSV di
Malaysia belum pernah dilaporkan. Kami mengkaji ciri-ciri genetik RSV daripada
spesimen yang dikumpulkan bermula dari bulan Mac hingga bulan Ogos 2011 dengan
cara pengesanan molekular. Daripada jumlah sebanyak 130 spesimen swab tekak dan
cecair nasofarink, 54 (41.5%) adalah RSV positif selepas diuji
dengan asai dalaman masa nyata transkriptase balik tindak balas berantai
polimerase (rRT-PCR). Tiga puluh empat daripada 54
(63.0%) pesakit dengan RSV positif adalah kanak-kanak di
bawah dua tahun dan dua (1.4%) adalah dewasa. Analisis filogenetik menunjukkan
bahawa 39 virus RSV adalah genotip GA5,
13 genotip GA2, satu genotip GA1 dan satu genotip GA7.
Hasil penyelidikan ini menunjukkan empat genotip RSV yang
sedang merebak di negara kita dan genotip utama adalah GA5.
Kata
kunci: Analisis filogenetik; genotip GA1, GA2, GA5 dan GA7; virus pernafasan sinsitium
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*Corresponding author; email: mmr@ppukm.ukm.edu.my
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