Sains Malaysiana 47(11)(2018): 2693–2698

http://dx.doi.org/10.17576/jsm-2018-4711-12

 

(-)-Glaciantarcin, a New Dipeptide and Some Secondary Metabolites from the Psychrophilic Yeast Glaciozyma antarctica PI12

((-)-Glasiantarsin, satu Dipeptida Terbaharu dan Beberapa Metabolit Sekunder daripada Yis Psikrofilik Glaciozyma antarctica PI12)

 

ANDI R. ROSANDY¹, YEOH CHIANN YING³, CHEAH YOKE KQUEEN³, SENG JOE LIM¹, JALIFAH LATIP¹, A. MUNIR A. MURAD², MUNTAZ A. BAKAR¹ & ROZIDA M. KHALID^1*

 

¹School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

²School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

³Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

 

Received: 26 March 2018/Accepted: 6 July 2018

 

ABSTRACT

A new dipeptide, (-)-glaciantarcin (1) and three known compounds, cyclo(-Pro-Gly) (2), 1-(2-deoxypentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione (3) and vidarabine (Ara-A) (4), were isolated from Glaciozyma antarctica PI12, a cold-adapted yeast. The chemical structures were elucidated by FT-IR, NMR and mass spectrometry. The cytotoxicity and antioxidant activities of compounds 1-4 were evaluated by using the MTT bioassay on MCF-7 (human breast cancer cell line), PC-3 (human prostate cancer cell line) and HEK-293 (normal human embryonic kidney cell line) and DPPH free radical scavenging activity, respectively. At concentration of 400 μM, all compounds showed the highest activity on MCF-7, with compound 1 at 65%, compound 2 (70%), compound 3 (66%) and compound 4 (58%) cell viability. All compounds exhibited weak antioxidant properties. To the best of our knowledge, this is the first report of compounds 1-4 from Glaciozyma antactica.

 

Keywords: Biological activities; dipeptide; (-)-glaciantarcin; Glaciozyma antarctica PI12; psychrophilic yeast

 

ABSTRAK

Satu dipeptida terbaharu iaitu (-)-glasiantarsin (1) dan tiga sebatian yang telah diketahui; siklo (-Pro-Gli) (2), 1- (2-Deoksipentofuranosil) -5-metil-2,4 (1H, 3H) -pirimidinedion (3) dan vidarabin (Ara-A) (4) telah diasingkan daripada Glaciozyma antarctica PI12, yis yang teradaptasi sejuk. Struktur kimia dijelaskan oleh spektroskopi inframerah (IM), ultra lembayung (UL), resonans magnetik nuklear (RMN) dan spektroskopi jisim. Aktiviti kesitotoksikan dan antioksidan daripada sebatian 1-4 dinilai menggunakan bioasai MTT ke atas MCF-7 (sel kanser payudara manusia), PC-3 (sel kanser prostat manusia) dan sel HEK-293 (sel normal) serta aktiviti perencatan radikal bebas DPPH. Pada kepekatan 400 μM, semua sebatian menunjukkan aktiviti tertinggi ke atas MCF-7, iaitu masing-masing: sebatian 1 (62 ± 0.9), 2 (66 ± 1.6), 3 (62 ± 1.3) dan 4 (55 ± 1.7). Kesemua sebatian terpencil menunjukkan sifat antioksidan yang lemah. Sepanjang pengetahuan kami, ini merupakan laporan pertama sebatian 1-4 daripada Glaciozyma antactica.

 

Kata kunci: Aktiviti biologi; dipeptida; (-)-glasiantarsin; Glaciozyma antarctica PI12; yis psikrofilik

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*Corresponding author; email: rozidakhalid@ukm.edu.my