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Sains Malaysiana 47(7)(2018): 1535–1540
http://dx.doi.org/10.17576/jsm-2018-4707-22
Comparison of DNA Profiling
between Fishes and Pork Meat using Polymerase Chain Reaction-Restriction
Fragment Length Polymorphisms (PCR-RFLP) Analysis
(Perbandingan Profil DNA antara Ikan dan Daging Babi menggunakan Analisis Tindak Balas
Rantaian Polimerase-Polimorfisme Panjang Cebisan
Pemotongan (PCR-RFLP))
SAFIYYAH SHAHIMI, SAHILAH ABD. MUTALIB*, WAN SAKEENAH WAN NAZRI, AMINAH ABDULLAH
& NORRAKIAH ABDULLAH SANI
School of Chemical Sciences and Food
Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600
UKM Bangi, Selangor Darul Ehsan, Malaysia
Received: 14 September 2017/Accepted: 23
February 2018
ABSTRACT
Genomic DNA of
13 fish (n=13) species consist of four freshwater which were catfish (Clarias
gariepinus), shark catfish (Pangasius larnaudii), tilapia
(Oreochromis mossambicus), perch (Lates calcarifer)
and nine marine species which were black pomfret (Parastromateus
niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger
kanagurta), red snapper (Lutjanus erythropterus), herring
(Chirocentrus dorab), ray fish (Himantura gerrardii),
sardine (Decapterus macrosoma), mackerel (Euthynnus
affinis) and tuna (Thunnus tuna) were differentiated
using polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP).
Seven endonucleases of AluI, BsaJI, HaeIII,
HindIII, HinfI, MboI and MboII were
examined for the ability to digest cyt b amplicon from
each species. Genomic DNA
of pork (Sus scrofa domestica) were differentiated
from fishes by comparing the digestion patterns produced by similar
amplified region and enzymes used. In the present study, it was
demonstrated that fishes and pork DNA genome
were successfully differentiated using all endonucleases except
for HindIII. Thus, PCR-RFLP analysis was found useful for future pork DNA
detection in fish products.
Keywords: DNA profiling;
fish; PCR-RFLP; pork (Sus scrofa domestica)
ABSTRAK
DNA genomik 13
spesies ikan (n=13) terdiri daripada empat ikan air tawar iaitu keli (Clarias
gariepinus), patin (Pangasius larnaudii), tilapia (Oreochromis
mossambicus), siakap (Lates calcarifer) dan sembilan
ikan air masin iaitu bawal hitam (Parastromateus niger),
ikan bilis (Stolephorus commersonii), mabong (Rastrelliger
kanagurta), ikan merah (Lutjanus erythropterus), ikan
parang (Chirocentrus dorab), pari (Himantura gerrardii),
sardin (Decapterus macrosoma), tenggiri (Euthynnus
affinis) dan tuna (Thunnus tuna) telah dibezakan menggunakan
tindak balas rantaian polimerase-polimorfisme panjang cebisan
pemotongan (PCR-RFLP).
Tujuh endonuklease iaitu AluI, BsaJI, HaeIII,
HindIII, HinfI, MboI dan MboII telah
dinilai kebolehannya bagi mencernakan amplikon cyt b daripada
setiap spesies. DNA genomik babi (Sus scrofa domestica) telah dibezakan
daripada ikan dengan membandingkan corak pencernaan yang terhasil
ke atas kawasan amplifikasi yang serupa menggunakan enzim yang
digunakan. Dalam kajian ini, DNA genomik ikan dan babi telah berjaya dibezakan oleh kesemua
endonuklease kecuali enzim HindIII.
Kata kunci: Babi (Sus scrofa domestica); ikan; PCR-RFLP
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*Corresponding author; email: sahilah@ukm.edu.my
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