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http://dx.doi.org/10.17576/jsm-2019-4810-21
Role of Different Salmonella enterica Serotypes in THP-1 Macrophage-Derived Foam Cell
Autophagy and Inflammatory Response (Peranan Serotip Berbeza Salmonella enterica pada Tindak Balas Sel
Buih THP-1 Makrofaj Terbitan Autofagi dan Inflamasi) MIN YI WONG1,
YUAN-HSI
TSENG1,
TSUNG-YU
HUANG2,
CHENG-HSUN
CHIU3,4,
CHYI-LIANG
CHEN3
& YAO-KUANG HUANG1* 1Division of Thoracic
and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital,
Puzi City, Chiayi County 613, and College of Medicine, Chang
Gung University, Taoyuan City 333, Taiwan (R.O.C.) 2Division of Infectious
Diseases, Department of Internal Medicine, Chang Gung Memorial Hospital,
Chang Gung University College of Medicine, Taoyuan City 333, Taiwan
(R.O.C.) 3Molecular Infectious
Disease Research Center, Chang Gung Memorial Hospital, Taoyuan City
333, Taiwan (R.O.C.) 4Division of Pediatric
Infectious Diseases, Department of Pediatrics, Chang Gung Children’s
Hospital and Chang Gung University, Taoyuan City 333, Taiwan (R.O.C.) Received: 22 January 2019/Accepted:
12 September 2019 ABSTRACT Infected
aortic aneurysm is a rare but life-threatening condition. Intimal
injury, especially in arteries with atherosclerotic plaques, is
vulnerable to Salmonella
species. In this study, THP-1 macrophage-derived foam cells were
used to mimic the atherosclerotic plaques. We investigated the role
of different Salmonella serotype infections in foam cell
autophagy and inflammatory response. Three different serotypes of
Salmonella enterica-Typhimurium, Enteritidis,
and Choleraesuis - with or without
a virulence plasmid were used in this study. The foam cell model
was established and observed under confocal microscopy. The expression
of autophagy and inflammasome-related proteins after Salmonella infection
was detected by Western blotting, and the secretion of the proinflammatory
cytokines interleukin-1β and IL-18 was detected using ELISA.
The plasmid-bearing S. enteritidis
OU7130-
and plasmid-less S. choleraesuis OU7266-infected
foam cells both showed a lower expression of beclin-1 at 0.5 h post-infection
(hpi) and p62 at 2 hpi as well as
a higher expression of caspase-1 at 2 hpi
than other infected cells. Moreover, infection of OU7266 induced the highest IL-1β
and IL-18 production among the tested strains. In conclusion,
the virulence plasmid of S. enteritidis
OU7130
induced foam cell inflammatory responses, whereas that of S.
choleraesuis played an opposite role. The different serotype
of Salmonella may impact the infected aortic aneurysms through
regulating inflammatory balance of the macrophage and foam cells
in the atherosclerotic plaque. Keywords:
Autophagy; foam cells; Salmonella serotypes; virulence
plasmid ABSTRAK Aneurisma aortik
yang terjangkit adalah
keadaan yang jarang berlaku namun boleh
mengancam nyawa.
Kecederaan intimal, terutamanya
dalam arteri dengan
plak atherosklerosis,
terdedah kepada spesies Salmonella. Dalam kajian ini,
sel buih yang
berasal daripada makrofaj THP-1 digunakan
untuk meniru
plak aterosklerotik. Kami mengkaji peranan jangkitan serotip Salmonella
yang berbeza dalam
autophagia sel buih dan tindak
balas keradangan.
Tiga serotip Salmonella enterica - Typhimurium, Enteritidis dan Choleraesuis-dengan atau
tanpa kevirulenan
plasmid digunakan dalam kajian ini. Model sel buih telah
ditubuhkan dan
diperhatikan di bawah mikroskopi konflik. Ekspresi protein yang berkaitan
dengan autofagia dan inflamasom selepas jangkitan Salmonella
dikesan oleh blot Western,
dan rembesan
sitokin proinflamasi interleukin-1β
dan IL-18
dikesan menggunakan
ELISA.
Pembekuan plasmid S. enteritidis
OU7130-
dan sel-sel
buih yang dioksida S. choleraesuis OU7266 kedua-duanya
menunjukkan ungkapan
beclin-1 yang lebih rendah
pada 0.5 jam selepas jangkitan (hpi) dan p62 pada 2 hpi serta ungkapan
kaspase-1 yang lebih tinggi
pada 2 hpi daripada sel yang dijangkiti yang lain. Tambahan pula,
jangkitan OU7266
mendorong kepada
pengeluaran tertinggi IL-1β
dan IL-18 dalam
kalangan strain yang diuji.
Sebagai kesimpulan,
plasmid virulensi S. enteritidis
OU7130
bertindak balas
dengan keradangan sel busa, sedangkan
S. choleraesuis memainkan
peranan yang bertentangan.
Serotip Salmonella yang berlainan
boleh memberi
kesan kepada aneurisma
aortik yang dijangkiti
melalui mengawal keseimbangan radang makrofaj dan buih
di plak aterosklerotik. Kata kunci: Autofagi; kevirulenan
plasmid; sel buih; serotip
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