Sains Malaysiana 48(11)(2019): 2437–2449

http://dx.doi.org/10.17576/jsm-2019-4811-14

Diversity of Saprobic Fungi on Magnolia garrettii: Do Collecting Sites and Seasons Affect the Fungal Community?

(Kepelbagaian Kulat Saprob pada Magnolia garrettii: Adakah Tapak Pengumpulan dan Musim Mempengaruhi Komuniti Kulat?)

KODSUEB, R.¹* & LUMYONG, S.^2,3

¹Microbiology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand

²Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand

³Academy of Science, The Royal Society of Thailand, Bangkok 10300, Thailand

Received: 1 April 2019/Accepted: 15 August 2019

ABSTRACT

The diversity of saprobic fungi found on woody litter of Magnola garrettii collected from Phu Hin Rongkla National Park in Phitsanulok Province, Thailand during the wet and dry seasons, from July 2008 to April 2009, was studied, and the fungal diversity and community was compared with a previous study in Doi Suthep-Pui National Park in Chiang Mai Province. Based on morphological characteristics, 141 taxa were obtained and classified as 40 Ascomycetes, 5 Basidiomycetes, 42 lichens, one unidentified taxon and 53 anamorphic fungi. The number of taxa recovered indicated that Dry season samples supported a more diverse fungal community than samples collected in the wet season, for both collecting sites, although the common genera of fungi obtained during each season were similar. Distinct fungal communities of saprobic fungi collected from each site suggest that site characteristics affect the community composition. Samples from Phu Hin Rongkla National Park provided higher numbers of fungi (especially lichens) than were collected in the previous Doi Suthep-Pui National Park study with few species overlapping in the two sites.

Keywords: Fungal diversity; lignicolous fungi; magnoliacea; seasonal effect; site specific

ABSTRAK

Kepelbagaian kulat saprob yang terdapat pada sampah berkayu Magnola garrettii yang dikumpulkan dari Taman Negara Phu Hin Rongkla di Wilayah Phitsanulok, Thailand semasa musim basah dan kering, dari Julai 2008 hingga April 2009 telah dikaji dan kepelbagaian kulat dan komuniti dibandingkan dengan kajian sebelumnya di Taman Negara Doi Suthep-Pui di Wilayah Chiang Mai. Berdasarkan ciri morfologi, 141 taksonomi telah diperoleh dan dikelaskan sebagai 40 Ascomycetes, 5 Basidiomycetes, 42 liken, satu tidak dikenal pasti dan 53 kulat anamorfik. Bilangan takat yang pulih menunjukkan bahawa sampel musim Kering menyokong komuniti kulat yang lebih pelbagai daripada sampel yang dikumpul pada musim hujan, untuk kedua-dua lokasi pengumpulan, walaupun genus kulat umum yang diperoleh pada setiap musim adalah sama. Komuniti kulat yang berbeza daripada kulat saprob yang dikumpulkan dari setiap tapak menunjukkan bahawa ciri tapak mempengaruhi komposisi masyarakat. Sampel dari Taman Negara Phu Hin Rongkla menyediakan lebih banyak kulat (terutama liken) daripada yang dikumpulkan dalam kajian di Taman Negara Doi Suthep-Pui dengan spesies yang agak banyak bertindih di kedua-dua tapak.

Kata kunci: Kepelbagaian kulat; kesan musim; kulat lignikolus; magnoliacea; tapak khas

REFERENCES

Alias, S.A., Kuthubutheen, A.J. & Jones, E.B.G. 1995. Frequency of occurrence of fungi on wood in Malaysian mangroves. Hydrobiologia 295: 97-106.

Allen, R.B., Buchanan, P.K., Clinton, P.W. & Cone, A.J. 2000. Composition and diversity of fungi on decaying logs in a New Zealand temperate beech (Nothofagus) forest. Canadian Journal of Forest Research 30: 1025-1033.

Anonymous. 1995. JMP® Statistics and Graphics Guide. Version 3.1 of JMP, SAS Institute Inc., Cary, NC.

Bisby, G.R. 1943. Geographical distribution of fungi. The Botanical Review 9: 466-482.

Boddy, L. & Watkinson, S.C. 1995. Wood decomposition, higher fungi, and their role in nutrient redistribution. Canadian Journal of Botany 73: S1377-S1383.

Cai, L., Hyde, K.D. & Tsui, C.K.M. 2006. Genera of Freshwater Fungi. Fungal Diversity Research Series 17, Hong Kong: Fungal Diversity Press.

Carmichael, J.W., Kendrick, W.B., Conners, I.L. & Sigler, L. 1980. Genera of Hyphomycetes. Edmonton: University of Alberta Press.

Chatanon, L. 2001. Biodiversity of Ascomycetous fungi at Huai- Kha Khaeng Wildlife Sanctuary. M.S. Thesis. Kasetsart University, Thailand (in Thai) (Unpublished).

Costa, L.A. & Gusmao, L.F. 2015. Characterization saprobic fungi on leaf litter of two species of trees in the Atlantic Forest, Brazil. Brazilian Journal of Microbiology 46: 1027- 1035.

Crites, S. & Dale, M.R.T. 1998. Diversity an abundance of bryophytes, lichens, and fungi in relation to woody substrate and successional stage in aspen mixed wood boreal forests. Canadian Journal of Botany 76: 641-651.

Dice, L.R. 1945. Measures of the amount of ecologic association between species. Ecology 26: 297-302.

Doilom, M., Dissanayake, A.J., Wanasinghe, D.N., Boonmee, S., Liu, J-K., Bhat, D.J., Taylor, J.E., Bahkali, A.H., McKenzie, E.H.C. & Hyde, K.D. 2017. Microfungi on Tectona grandis (teak) in Northern Thailand. Fungal Diversity 82: 107-182.

ElHadj, A.I.B., Zaouali, Y., Bejaoui, A. & Boussaid, M. 2010. Variation of the chemical composition of essential oils in Tunisian populations of Thymus algeriensis Boiss. et Reut. (Lamiaceae) and implication for conservation. Chemistry & Biodiversity 7: 1276-1289.

Ellis, M.B. 1976. More Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew.

Ellis, M.B. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew.

Hanlin, R.T. 1998. Illustrated Genera of Ascomycetes, Vol II. Minnesota: APS Press.

Hanlin, R.T. 1990. Illustrated Genera of Ascomycetes. Minnesota: APS Press.

Hawksworth, D.L. 2002. Why study tropical fungi. In Tropical Mycology, Vol. 2, Micromycetes, edited by Watling, R., Frankland, J.C., Ainsworth, A.M., Isaac, S. & Robinson, C.H.). Wallingford: CABI Publishing. pp. 1-11.

Ho, W.H., Yanna, Hyde, K.D. & Hodgkiss, I.J. 2002. Seasonality and sequential occurrence of fungi on wood submerged in Tai Po Kau Forest Stream, Hong Kong. In Fungal Succession, edited by Hyde, K.D. & Jones, E.B.G.), Hong Kong: Fungal University Press. pp. 21-43.

Holmer, L. & Stenlid, J. 1996. Diffuse competition among wood decay fungi. Oecologia 106: 531-538.

Huhndorf, S.M. & Lodge, D.J. 1997. Host specificity among wood-inhabiting pyrenomycetes (fungi, ascomycetes) in a wet tropical forest in Puerto Rico. Tropical Ecology 38: 307-315.

Hyde, K.D. & Lee, S.Y. 1995. Ecology of mangrove fungi and their role in nutrient cycling. What gaps occur in our knowledge? Hydrobiologia 295: 107-118.

Hyde, K.D., Taylor, J.E. & Frohlich, J. 2000. Genera of Ascomycetes from Palms. Fungal Diversity Research Series 2. Hong Kong: Fungal Diversity Press.

Hyde, K.D., Bussaban, B., Paulus, B., Crous, P.W., Lee, S., Mckenzie, E.H.C., Photita, W. & Lumyong, S. 2007. Diversity of saprobic microfungi. Biodiversity and Conservation 16: 7-35.

Inderbitzin, P. & Berbee, M.L. 2001. Lollipopaia minuta from Thailand, a new genus and species of the Diaporthales (Ascomycetes, Fungi) based on morphological and molecular data. Canadian Journal of Botany 79: 1099-1106.

Inderbitzin, P., Landvik, S., Abdel-Wahab, M.A. & Berbee, M.L. 2001. Aliquandostipitaceae, a new family for two new tropical ascomycetes with unusually wide hyphae and dimorphic ascomata. American Journal of Botany 88: 52-61.

Kane, D.F., Tam, W.Y. & Jones, E.B.G. 2002. Fungi colonizing and sporulating on submerged wood in the River Severn, U.K. In Fungal Succession, edited by Hyde, K.D. & Jones, E.B.G. Hong Kong: Fungal University Press. pp. 45-55.

Kennedy, N., Brodie, E., Connolly, J. & Clipson, N. 2006. Seasonal influences on fungal community structure in unimproved and improved upland grassland soils. Canadian Journal of Microbiology 52: 689-694.

Khattak, K.F. & Rahman, T.R. 2015. Effect of geographical distributions on the nutrient composition, phytochemical profile and antioxidant activity of Morus nigra. Pakistan Journal of Pharmaceutical Science 28: 1671-1678.

Kiffer, E. & Morelet, M. 1999. The Deuteromycetes, Mitosporic fungi: Classification and Generic Keys. Enfield: Science Publishers.

Kodsueb, R. & Lumyong, S. 2018. Antimicrobial properties and detection of crude extract derived from Magnolia garrettii inhabiting fungi. NU. International Journal of Science 15: 49-68.

Kodsueb, R., Lumyong, S., Ho, W.H., Hyde, K.D., McKenzie, E.H.C. & Jeewon, R. 2007a. Morphological and molecular characterization of Aquaticheirospora and phylogenetics of Massarinaceae (Pleosporales). Botanical Journal of the Linnean Society 155: 283-296.

Kodsueb, R., McKenzie, E.H.C., Ho, W.H., Hyde, K.D., Lumyong, P. & Lumyong, S. 2007b. New anamorphic fungi from decaying woody litter of Michelia baillonii (Magnoliaceae) in northern Thailand. Cryptogamie Mycologie 28: 237-245.

Kodsueb, R., Lumyong, S., Hyde, K.D., Lumyong, P. & McKenzie, E.H.C. 2006. Acrodictys micheliae and Dictyosporium manglietiae, two new anamorphic fungi from woody litter of Magnoliaceae in northern Thailand. Cryptogamie Mycologie 27: 111-119.

Kodsueb, R., McKenzie, E.H.C., Lumyong, S. & Hyde, K.D. 2008. Diversity of saprobic fungi on Magnoliaceae. Fungal Diversity 30: 37-53.

Lee, B.H., Kwon, W.J., Kim, J.Y., Park, J.S. & Eom, A.H. 2017. Differences among endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from different sites in Korea. Microbiology 45: 312-317.

Ling, O.M., Teen, L.P., Mujahid, A., Proksch, P. & Muller, M. 2016. Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential. Sains Malaysiana 45(7): 1063-1071.

Liu, Y., Chen, P., Zhou, M., Wang, T., Fang, S., Shang, X. & Fu, X. 2018. Geographic variation in the chemical composition and antioxidant properties of phenolic compounds from Cyclocarya paliurus (Batal) Iljinskaja leaves. Molecules 23: 2440.

Liu, W., Liu, J., Yin, D. & Zhao, X. 2015. Influence of ecological factors on the production of active substances in the anti-cancer plant Sinopodophyllum hexandrum (Royle) T.S. Ying. PLoS ONE 10: e0122981.

Lodge, D.J. 1997. Factors related to diversity of decomposer fungi in tropical forests. Biodiversity and Conservation 6: 681-688.

Lu, B. & Hyde, K.D. 2000. A World Monograph of Anthostomella. Fungal Diversity Research Series 4. Hong Kong: Fungal Diversity Press.

Magurran, A.E. 2004. Measuring Biological Diversity. Blackwell Publishing, Oxford.

Maria, G.L. & Sridhar, K.R. 2004. Fungal colonization of immersed wood in mangroves of the southwest coast of India. Canadian Journal of Botany 82: 1409-1418.

McCune, B. & Mefford, M.J. 1999. PC-ORD: MultivariateAanalysis of Ecological Data. Version 4 [computer program]. MjM software Design, Gleneden Beach, Oregon.

Monkai, J., Promputtha, I., Kodsueb, R., Chukeatirote, E., McKenzie E.H.C. & Hyde, K.D. 2013. Fungi on decaying leaves of Magnolia liliifera and Cinnamomum iners show litter fungi to be hyperdiverse. Mycosphere 4: 292-301.

Nag Raj, T.R. 1993. Coelomycetous Anamorphs with Appendage-bearing Conidia. Mycological Paper, Waterloo.

Nikolcheva, L.G. & Barlocher, F. 2005. Seasonal and substrate preferences of fungi colonizing leaves in streams: Traditional versus molecular evidence. Environmental Microbiology 7: 270-280.

Pinnoi, A., Jeewon, R., Sakayaroj, J., Hyde, K.D. & Jones, E.B.G. 2007. Berkleasmium crunisia sp. nov. and its phylogenetic affinities to the Pleosporales based on 18S and 28S rDNA sequence analyses. Mycologia 99: 378-384.

Pinnoi, A., Lumyong, S., Hyde, K.D. & Jones, E.B.G. 2006. Biodiversity of fungi on the palm Eleiodoxa conferta in Sirindhorn peat swamp forest, Narathiwat, Thailand. Fungal Diversity 22: 205-218.

Promputtha, I., Lumyong, S., Lumyong, P., McKenzie, E.H.C. & Hyde, K.D. 2005. A new species of Anthostomella on Magnolia liliifera from northern Thailand. Mycotaxon 91: 413-418.

Promputtha, I., Hyde, K.D., Lumyong, P., McKenzie, E.H.C. & Lumyong, S. 2004a. Fungi on Magnolia liliifera: Cheiromyces magnoliae sp. nov. from dead branches. Nova Hedwigia 80: 527-532.

Promputtha, I., Lumyong, S., Lumyong, P., McKenzie, E.H.C. & Hyde, K.D. 2004b. A new species of Pseudohalonectria from Thailand. Cryptogamie Mycologie 25: 43-47.

Promputtha, I., Lumyong, S., Lumyong, P., McKenzie, E.H.C. & Hyde, K.D. 2004c. Fungal saprobes on dead leaves of Magnolia liliifera (Magnoliaceae) in Thailand. Cryptogamie Mycologie 25: 315-321.

Promputtha, I., Hyde, K.D., Lumyong, P., McKenzie, E.H.C. & Lumyong, S. 2003. Dokmaia monthadangii gen. et sp. nov., a synnematous anamorphic fungus on M. garrettii. Sydowia 55: 99-103.

Rattanathirakul, W. & Boonkerd, T. 2003. Taxonomy of ferns and fern allies at Phu Hin Rong Kla national park, Phitsanulok province. BRT 2003 Research Reports. pp. 58-67.

Rayner, A.D.M. & Boddy, L. 1988. Fungal Decomposition of Wood and Its Biology and Ecology. Chichester: John Wiley and Sons.

Rayner, A.D.M. & Todd, N.K. 1979. Population and community structure and dynamics of fungi in decaying wood. Advance in Botanical Research 7: 333-420.

Renvall, P. 1995. Community structure and dynamics of wood-rotting basidiomycetes on decomposing conifer trunks in northern Finland. Karstenia 35: 1-51.

Sarma, V.V. & Vittal, B.P.R. 2000. Biodiversity of mangrove fungi on different substrata of Rhizophora apiculata and Avicennia spp. from Godavari and Krishna deltas, east coast of India. In Aquatic Mycology across the Millennium, edited by Hyde, K.D. Ho, W.H. & Pointing, S.B. Fungal Diversity 5: 23-41.

Schumacher, T. 1982. Ascomycetes from northern Thailand. Nordic Journal of Botany 2: 257-263.

Seephueak, P., Petcharat, V. & Phongpaichit, S. 2010. Fungi associated with leaf litter of para rubber (Hevea brasiliensis). Mycology 1: 213-227.

Seephueak, P., Phongpaichit, S., Hyde, K.D. & Petcharat, V. 2011. Diversity of saprobic fungi on decaying branch litter of the rubber tree (Hevea brasiliensis). Mycosphere 2: 307-330.

Seifert, K., Jones, M.G., Gams, W. & Kendrick, B. 2011. The Genera of Hyphomycetes. CBS biodiversity series no. 9: 1-997. Utrecht: CBS-KNAW Fungal Biodiversity Centre.

Shannon, C.E. & Weaver, W. 1949. The Mathematical Theory of Communication. Urbana: University of Illinois Press.

Shearer, C.A. 1992. The role of woody debris. In The Ecology of Aquatic Hyphomycetes, edited by Bärlocher, F. Ecological Studies 94. Berlin: Springer-Verlag. pp. 77-98.

Sihanonth, P., Thienhirun, S. & Whalley, A.J.S. 1998. Entonaema in Thailand. Mycological Research 102: 458-460.

Sivanesan, A. 1984. The Bitunicate Ascomycetes and their Anamorphs. Germany: Cramer, Vaduz.

Sivichai, S., Jones, E.B.G. & Hywel-Jones, N. 2002. Fungal colonization of wood in a freshwater stream at Tad Ta Phu, Khao Yai National Park, Thailand. In Fungal Succession, edited by Hyde, K.D. & Jones, E.B.G. Hong Kong: Fungal University Press. pp. 113-129.

Sørensen, T. 1948. A method of establishing groups of equal amplitude in plant sociology based on similarity of species and its application to analyses of the vegetation on danish commons. Biologiske Skrifter/Kongelige Danske Videnskabernes Selskab 5: 1-34.

Supasri, T., Phuphisith, S. & Sampattagul, S. 2018. Monitering and evaluation of PM2.5/10 in Chiang Mai Province by DustBoy sensors. International Workshop: Environmental Health Issues and Research Collaboration between Thailand and Korea. Northern Science Park, Chiang Mai. 9-10 August.

Sutton, B.C. 1980. The Coelomycetes. Kew, UK: Commonwealth Mycological Institute.

Tan, T.K., Leong, W.F. & Jones, E.B.G. 1989. Succession of fungi on wood of Avicennia alba and A. lanata in Singapore. Canadian Journal of Botany 67: 2687-2691.

Taylor, J.E. & Hyde, K.D. 2003. Microfungi of Tropical and Temperate Palms. Fungal Diversity Research Series 12. Hong Kong: Fungal Diversity Press.

Teoh, Y.P. & Don, M.M. 2013. In vitro antifungal activities and phytochemical analysis of filamentous white-rot fungi, Schizophyllum commune. Sains Malaysiana 42(9): 1267- 1272.

Thienhirun, S. 1997. A preliminary account of the Xylariaceae of Thailand. Ph.D. Thesis. Liverpool John Moores University, U.K. (Unpublished).

Thienhirun, S., Rodtong, S., Phukhawan, N. & Suwannasai, N. 2003. Xylariaceous fungus in Phu Hin Rongkla National Park, Thailand. Technology for Life, Thailand: Pattaya Exhibition and Conventional Hall. July 17-21.

Thongkantha, S., Lumyong, S., McKenzie, E.H.C. & Hyde, K.D. 2008. Fungal saprobes and pathogens occurrence on tissues of Dracaena loureiri and Pandanus spp. Fungal Diversity 30: 149-179.

Tibpromma, S., Bhat, J.D., Doilom, M., Lumyong, S., Nontachaiyapoom, S., Yang, J-B. & Hyde, K.D. 2016. Three new Hermatomyces species (Lophiotremataceae) on Pandanus odorifer from Southern Thailand. Phytotaxa 275: 127-139.

Van Ryckegem, G. & Verbeken, A. 2005. Fungal diversity and community structure on Phragmites australis (Poaceae) along a salinity gradient in the Scheldt estuary (Belgium). Nova Hedwigia 80: 173-197.

Wu, W.P. & Zhuang, W. 2005. Sporidesmium, Endophragmiella and related genera from China. Fungal Diversity Research Series 15, Hong Kong: Fungal Diversity Press.

Zhou, D. & Hyde, K.D. 2001. Host-specificity, host-exclusivity, and host-recurrence in saprobic fungi. Mycological Research 105: 1449-1457.

Zouari, N., Ayadi, I., Fakhfakh, N., Rebai, A. & Zouari, S. 2012. Variation of chemical composition of essential oils in wild populations of Thymus algeriensis Boiss. et Reut., a North African endemic species. Lipids in Health and Disease 11: 28.

*Corresponding author; email: rampai.k@psru.ac.th