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Sains Malaysiana 49(1)(2020): 19-27
http://dx.doi.org/10.17576/jsm-2020-4901-03
PEGylated
Oleic Acid-Lecithin Liposomes (POLL) for Anticancer Drug Delivery
(Liposom
Asid Olik-Lesitin BerPEG (POLL) untuk Penghantar Ubat Anti Barah)
VICIT
RIZAL EH SUK1*, IVY CHUNG2 & MISNI MISRAN1
1Colloid Laboratory, Department of
Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur,
Federal Territory, Malaysia
2Department of Pharmacology, Faculty of
Medicine, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia
Received:
6 March 2019/Accepted: 8 October 2019
ABSTRACT
Cancer is a major health issue, conferring
to more than 14.5 million deaths worldwide. Liposomes, self-assembly
amphiphilic bilayer molecules, served as excellent alternative vehicles due to
their ability to encapsulate both hydrophobic and hydrophilic anticancer drugs.
Conventional liposomes, comprised mainly phospholipids are cost-ineffective,
unstable, and easily degraded by the external environment. In this study, we
introduced PEGylated oleic acid-lecithin liposomes constructed by using C-18
monounsaturated fatty acids (oleic acid) and soy lecithin, in the presence of
DOPEPEG2000 in pH7.4, above their glass transition temperature, Tg, by employing the simple thin layer lipid
hydration method. FTIR spectrum of oleic acid, soy lecithin, and DOPEPEG2000
was studied. The average particle size without further mechanical interference
was 1102.3 nm while the zeta potential value was -18 mV, which is compatible
with the zeta potential of the red blood cell. The polydispersity index (PDI)
was reduced by 46.2% with the incorporation of the DOPEPEG2000. The
morphological study using OPM showed the presence of spherical shape liposomes
that exhibit the birefringence effect under the light field and Maltese cross
under the dark field. Encapsulation of folinic acid, methotrexate, doxorubicin,
or irinotecan resulted in greater than 75% encapsulation efficiency (EE).
Half-maximal inhibitory concentration, IC50, was significantly reduced in POLL as compared to free anticancer
drugs. Our data demonstrate POLL may be a promising alternative vehicle to
deliver various anticancer drugs to targeted tumour sites.
Keywords: Anticancer drugs; liposomes;
oleic acid; POLL; soy lecithin
ABSTRAK
Barah merupakan isu kesihatan utama yang menyebabkan lebih 14.5 juta kematian
di seluruh dunia. Liposom, iaitu molekul swabentuk dwilapis merupakan
penghantar alternatif terbaik berikutan kebolehannya untuk mengkapsulkan ubatan
anti barah hidrofobik dan hidrofilik. Liposom yang lazimnya diperbuat daripada
fosfolipid adalah tidak efektif daripada segi kos, tidak stabil dan mudah
terurai oleh persekitaran luar. Dalam kajian ini, kami memperkenalkan liposom
asid olik-lesitin berPEG yang terdiri daripada asid lemak tak tepu C-18 (asid
olik) dan lesitin soya, dengan kehadiran DOPEPEG2000 dalam pH7.4, pada suhu
lebih tinggi daripada suhu peralihan gelas Tg, menggunakan metod hidrasi lapisan nipis
lipid. Spektrum FTIR asid olik, lesitin soya, dan DOPEPEG2000 juga turut
dikaji. Purata saiz partikel adalah 1102.3 nm manakala keupayaan zeta adalah
-18 mV, sesuai dengan
keupayaan zeta sel darah merah. Indeks polisebaran telah berkurang sebanyak
46.2% dengan penambahan DOPEPEG2000. Kajian morfologi menggunakan Mikroskop
Optikal Pengutuban menunjukkan kewujudan liposom berbentuk sfera yang
menghasilkan kesan dwibiasan pada medan cerah dan silang Maltese pada medan
gelap. Pengkapsulan asid folinik, metotreksat, doksorubisin atau irinotecan
menghasilkan lebih 75% kecekapan pengkapsulan. Kepekatan separa rencatan, IC50, telah berkurang dengan signifikan pada POLL berbanding ubatan anti barah. Data menunjukkan POLL mungkin
berpotensi untuk menjadi penghantar ubatan anti barah
ke tempat yang ditujui.
Kata kunci: Asid olik; lesitin soya; liposom; POLL; ubatan anti barah
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*Corresponding
author; email: vicitrizal@um.edu.my
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