Sains Malaysiana 48(6)(2019): 1239–1249

http://dx.doi.org/10.17576/jsm-2019-4806-11

3-Dimensional Electric Field Distributions of Castellated and Straight Dielectrophoresis Electrodes for Cell Separation

(Pengagihan Medan Elektrik 3-Dimensi Elektrod Dielektroforesis Kekota dan Lurus untuk Pembahagian Sel)

FARAHDIANA WAN YUNUS, MUHAMMAD RAMDZAN BUYONG, JUMRIL YUNAS,

BURHANUDIN YEOP MAJLIS & AZRUL AZLAN HAMZAH*

Institute of Microenegineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Diserahkan: 13 Disember 2018/Diterima: 28 Februari 2019

ABSTRACT

This paper discusses the 3-dimensional (3D) electric field distributions on the surface and across the bulk volume of dielectrophoresis (DEP) electrodes. The importance of obtained high electric field is to ensure that biological particles will be able to separate even at low voltage potentials in order to avoid damage to the biological particles. Two electrodes - straight and castellated, were designed using the COMSOL Multiphysics Software Version 5.2 to compare the surface distribution and volume electric fields along the x, y and z axes. The results showed that castellated electrodes showcased higher electric fields for both the surface and volume factors along all axes. The maximum value of volume electric field results was 3.94×105 V/m along the x-axis, 3.80×105 V/m along the y-axis and 1.65×105 V/m along the z-axis. The maximum value of surface electric fields distributions was 3.39×105 V/m along the x-axis, 2.87×105 V/m along the y-axis and 1.14×105 V/m along the z-axis. Additionally, the uniformity of the electric field lines distribution from the COMSOL Multiphysics also indicated that castellated electrodes have a much higher uniformity. The experimental results showed that the castellated electrodes separated particles much faster at 69 s, as compared to straight electrodes at 112 s. Henceforth, this has proven that castellated electrodes have a high electric field as it separates much faster as compared to straight electrodes

.

Keywords: Biological particles; castellated electrodes; DEP; dielectrophoresis; electric fields; straight electrodes

ABSTRAK

Kajian ini membincangkan aliran medan elektrik 3-dimensi (3D) pada permukaan dan isi padu elektrod dieletroforesis (DEP). Medan elektik begitu penting bagi memastikan sel zarah biologi mampu untuk dipisahkan walaupun pada voltan yang rendah bagi mengelakkan kerosakan pada sel zarah tersebut. Dua elektrod, lurus dan kekota direka bentuk menggunakan perisian COMSOL Multiphysics versi 5.2 bagi membandingkan taburan medan elektrik di permukaan elektrod dan isi padu elektrod pada paksi x, y dan z. Keputusan menunjukkan bahawa elektrod kekota mempunyai medan elektrik yang tertinggi pada kedua-dua medan elektrik permukaan dan medan elektrik isi padu. Keputusan pada medan elektrik berisi padu adalah bernilai 3.94×105 V/m pada paksi x, 3.80×105 V/m pada paksi y dan 1.65×105 V/m pada paksi z, manakala keputusan pada medan elektrik permukaan adalah 3.39×105 V/m pada paksi x, 2.87×105 V/m paksi y dan 1.14×105 V/m pada paksi z. Tambahan pula, keputusan COMSOL Multiphysic juga menjelaskan keseragaman medan elektrik pada elektrod kekota adalah lebih tinggi. Keputusan menunjukkan elektrod kekota berjaya mengasingkan zarah dengan lebih cepat iaitu pada 69 s berbanding elektrod lurus pada 112 s. Oleh yang demikian, ini telah membuktikan bahawa elektrod kekota mempunyai daya medan elektrik yang tinggi setelah ia berjaya memisahkan sampel sel zarah tersebut lebih laju daripada elektrod lurus.

Kata kunci: DEP; dielektroforesis; elektrod kekota; elektrod lurus; medan elektrik; zarah biologi

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*Pengarang untuk surat-menyurat; email: azlanhamzah@ukm.edu.my