The Malaysian Journal of Analytical Sciences Vol 10 No 1 (2006): 137 – 142

 

 

 

 

A PRELIMINARY INVESTIGATION ON A HISTAMINE BIOSENSOR CONSTRUCTED  FROM DIAMINE OXIDASE IMMOBILISED ONTO AN OXYGEN PROBE

 

Norazlina Othman, Fatimah Abu Bakar¹, Abu Bakar Salleh¹, Lee Yook Heng²*

and Rahman Wagiran¹

 

¹Institute of Bioscience, Universiti Putra Malaysia

43400 Serdang, Selangor, Malaysia

² School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

 

*Correspondence author:  yhl1000@pkrisc.cc.ukm.my

 

Abstract

A  histamine  biosensor  consisting  of  diamine  oxidase  (DAO)  and  a  conventional  oxygen  electrode transducer was developed and applied for the determination of standard histamine solutions. Two different approaches for the histamine biosensor design were studied, i.e. the enzyme DAO was directly immobilized on the surface of the oxygen electrode membrane using glutaraldehyde or entrapped in a hydrogel film. For immobilisation with glutaraldehyde, the enzyme was cross-linked with glutaraldehyde as a bifunctional reagent on the electrode surface.  For entrapment, DAO was entrapped in a polymeric hydrogel film, i.e. poly(hydroxyl ethyl methacrylate) (pHEMA) polymer and deposited onto the teflon membrane of the oxygen electrode. The response of the histamine biosensors with immobilized DAO showed good linear correlation between the changes of oxygen level with changes in concentration of histamine at both high concentration ranges (200-1000 mg/L) and low concentrations (20-100 mg/L). However, at high concentration range of histamine, the sensitivity of the biosensor response decreased for the direct DAO immobilisation with glutaraldehyde.

 

References

1.     Halasz  A.,  Barats  A.,  Simon-Sardaki  L.  and  Holzapfen  W.(1994).  Trends  in  Food  Science  and Technology.  5, 42.

2.     Yano Y., Yokoyama K., Tamiya E. and Karube I.(1996). Anal. Chim. Acta, 64, 584.

3.     Hui J. Y. and Taylor S. L.(1983). High pressure liquid chromatographic determination of putrefactive amines in foods, Journal of The Association of Official Analytical Chemist, 66, 853-857.

4.     Rosier  J.  and Peteghem C. V.(1988). A screening method for the simultaneous determination of putrescine, cadaverine, histamine, spermidine and spermine in fish by means of high pressure liquid chromatography    of their 5-dimethylaminonapthalene-1-sulphonyl    derivatives,   Zeitschrift   fur Lebensmittel Untersuchung und Forschung, 186, 25-28.

5.   Yen G. C. and Hsieh C. L. (1991). Simultaneous analysis of biogenic amines in canned fish by HPLC, Journal of Food Science, 56, 158-160.

6.     Draisci  R.,  Cavalli  S.,  Lucentini  L.,  Stacchini  A.  (1993).  Ion  exchange  separation  and  pulsed amperometric detection for determination of biogenic amines in fish products, Chromatographia, 35, 584-590.

7.     Karube I., Satoh I., Araki Y. and Suzuki S.(1980). Monoamine oxidase electrode in freshness testing of meat, Enzyme and Microbial Technology, 2, 117-120.

8.     Toul Z., Macholan L. (1975).  Coll. Czech. Chem. Commun, 40, 2208.

9.     Botre F., Botre C., Laurenti G. and Mazzei F. (1993). Plant tissue biosensors for the determination of biogenic diamines and their amino acid precursor: effect of carbonic anhydrase, Sensors and Actuators B, 15 &16, 135-140.

10. D’Souza S. F. (2006) Immobilization and stabilization of biomaterials for biosensor applications, Appl. Biochem. Biotechnol. (in press).

11.  Bickerstaff G.F. (1997). Immobilization of Enzymes and Cells, Humana Press, Totowa, NJ.

12.  D’Souza S. F. (1989). Immobilized cells: techniques and applications”, Indian J. Microbiol. 29, 83- 117.

13.  Buffoni F. (1966). Histaminase and related amine oxidase, Pharmacol. Rev. 18, 1163-1199.

14. Mondovi B., Rotilio G, Costa M. T. and Agro A. F. (1971). Diamine oxidase (pig kidney)  In: Methods Enzymology (Eds. Tabor, H., Tabor, C. W.,) Academic Press, New York, XVII, Part B, 735-740.

15. Janes S. M., Palcic M. M., Scaman C. H., Smith A. J., Brown D. E., Mure D. M., Dooley M. and Klinman J. P. (1992). Identification of topaquinone and its consensus sequence in copper amine oxidases, Biochem, 31, 12147-12154.

16. Tombelli S. and Mascini M. (1998). Electrochemical biosensor for biogenic amines:a comparison between different approaches, Anal. Chim. Acta, 358, 277-284.

17. Bouvrette, P., Male, K.B., Luong, J.H.T. and Gibbs, B.F. (1997). Amperometric biosensor for diamine using diamine oxidase purified from porcine kidney. Enzyme Microbial Technology, 20, 32-38.

18.  Khor, S.M. (2004). Fabrikasi biopenderia sebatian fenol dan urea berasaskan pemegunan enzim dalam polimer metakrilat. MSc Thesis, School of Chemical Sciences & Food Technology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia.

19. Carsol, M.A. and Mascini, M. (1999). Diamine oxidase and putrescine oxidase immobilized reactors in flow injection analysis: a comparison in substrate specificity. Talanta, 50, 141-148.