The Malaysian Journal of Analytical Sciences Vol 12 No 2 (2008): 352 – 356
SELECTIVE ACCELERATED SOLVENT EXTRACTION FOR THE ANALYSIS OF SOIL POLYCYCLIC AROMATIC HYDROCARBONS AND STEROLS
Rozita Osman1*, Nor’ashikin Saim1, and Md Pauzi Abdullah2
1Faculty of Applied Sciences, Universiti Teknologi MARA 40450 Shah Alam, Selangor
2Faculty of Science and Technology,
Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia
*Corresponding author: rozit471@salam.uitm.edu.my
Abstract
Accelerated solvent extraction (ASE) has been successfully used in the analysis of a wide range of chemicals from many sample matrices. However, the main problem with accelerated solvent extraction is low selectivity towards the analyte because during the extraction process, many interfering components are co-extracted together with target analytes and thus requires post-extraction clean-up processes. In this study, a selective accelerated solvent extraction with clean-up step incorporated inside the extraction cell was developed for the analysis of polycyclic aromatic hydrocarbons (PAHs) and sterols in soil. PAHs (naphthalene, acenaphtene, anthracene, pyrene) and sterols (coprostanol, cholesterol, stigmasterol, stigmastanol) were extracted separately using two elution steps. The selectivity and efficiency of this approach were evaluated using several sorbents and proper choice of solvents. Using polar sorbents, PAHs were recovered in the first extraction using n-hexane while sterols were recovered in the second extraction using a more polar solvent such as methanol, isopropanol, acetone and mixture of DCM:MeOH (40:60, v/v). Recoveries for PAHs ranged from 76.5-99.2 % and sterols from 83.7.7-91.4% using silica as the sorbent, n-hexane as the first eluent, and methanol as the second eluent.
Keywords: Accelerated solvent extraction, polycyclic aromatic hydrocarbons, sterols.
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