The Malaysian Journal of Analytical Sciences Vol 14 No 1 (2010): 32 – 40

 

 

 

ANALYSIS OF ESSENTIAL OILS OF ETLINGERA SPHAEROCEPHALA VAR. GRANDIFLORA BY TWO-DIMENSIONAL GAS CHROMATOGRAPHY WITH TIME-OF-FLIGHT MASS SPECTROMETRY

 

M.A.A. Yahya¹, W.A. Yaacob¹, Laily B. Din¹ , I. Nazlina²

 

¹School of Chemical Sciences and Food Technology,

²School of Bioscience and Biotechnology,

Faculty of Science and Technology, Universiti Kebangsaan Malaysia,

43600 Bangi, Selangor, Malaysia.

 

Abstract

Hydrodistillation using a Clevenger-type apparatus of the Etlingera sphaerocephala var. grandiflora rhizomes, stem, leaves and whole plant yielded oils of respective 0.03, 0.02, 0.17 and 0.05%. Forty two compounds (or 97%), 32 (81%), 40 (63%) and 36 (80%) of the rhizome, stem, leaf and whole plant oils gave good matches in GCXGC-TOFMS analysis. The major components in the rhizome oil: 1,8-cineole (16.8%), α-phellandrene (12.7%) and β-trans-ocimene (8.9%); the stem: 1,8-cineole (17.4%), α-phellandrene (9.7%) and 1S-α-pinene (9.5%); the leaf: α-phellandrene (12.3%) and diprene (10.3%); and in the whole: β-pinene (12.2%), α-pinene (8.6%), p-menth-1-en-8-ol (8.5%) and α-phellandrene (8.5%). Monoterpenes constituted the richest components (76% average) in all of the four oils followed by sesquiterpenes (4%) and non-terpenes (0.2%). Three clusters of 1,8-cineole and α-phellandrene; β-pinene and 1,8-cineole; and α-phellandrene were obtained in cluster and principal component analyses.

 

Keywords: Etlingera sphaerocephala var. grandiflora, essential oils, GCXGC-TOFMS

 

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