Tissue Culture Laboratory

Model: Mettler Toledo

Brand: Mettler Toledo

Analytical Balance 4-D

Status: Available

The balance line comprises portable balances with a readability of 0.0001 g to 210 g.

An analytical balance is a highly accurate laboratory balance designed to determine an object’s mass precisely. The objects could be solid, liquid, granular substances, or powders. Analytical balances are extremely accurate laboratory balances created to precisely measure the mass of an object. Offering a readability of up to 0.00001 grams (0.01 mg), analytical balances are frequently used in laboratories. Providing such accurate measurements means that the balance is highly sensitive.

Analytical balance calculates weights based on the force required to balance the mass of a sample rather than utilizing actual masses. They produce a force to balance the sample using an electromagnet, then output the result by measuring the force required. A transparent enclosure with doors surrounds the measurement pan of an analytical balance (0.1 mg or greater), blocking external influences. As a result, dust does not collect, and air currents in the room do not affect the weighing performance.


Uses/ Applications of Analytical Balance

Analysis and determination of density
Preparation of sample
Pipette calibration
Differential weighing
Piece counting
Interval weighing
Formulation/ Recipe calculation
Percent weighing
Filling
Gross-net tare weighing
Checkweighing

Model: PL 4002

Brand: Mettler Toledo

Analytical Balance 2-D

Status: Available

Specification: The balance line comprises portable balances with a readability of 0.01 g to 4100 g.

An analytical balance is a highly accurate laboratory balance designed to determine an object’s mass precisely. The objects could be solid, liquid, granular substances, or powders. Analytical balances are extremely accurate laboratory balances created to precisely measure the mass of an object. Offering a readability of up to 0.00001 grams (0.01 mg), analytical balances are frequently used in laboratories. Providing such accurate measurements means that the balance is highly sensitive.

Analytical balance calculates weights based on the force required to balance the mass of a sample rather than utilizing actual masses. They produce a force to balance the sample using an electromagnet, then output the result by measuring the force required. A transparent enclosure with doors surrounds the measurement pan of an analytical balance (0.1 mg or greater), blocking external influences. As a result, dust does not collect, and air currents in the room do not affect the weighing performance.

Uses/ Applications of Analytical Balance

Analysis and determination of density
Preparation of sample
Pipette calibration
Differential weighing
Piece counting
Interval weighing
Formulation/ Recipe calculation
Percent weighing
Filling
Gross-net tare weighing
Check weighing

Model: Sorvall ST 16R

Brand: Thermo Fisher Scientific

Refrigerated Bench Top Centrifuge

Status: Available

The centrifuge is to be used for separating materials of different densities or particle sizes suspended in a liquid. A centrifuge is a device used to separate components of a mixture based on their size, density, viscosity of the medium, and the rotor speed.
The centrifuge is commonly used in laboratories for the separation of biological molecules from a crude extract.
There are different types of centrifuges used for the separation of different molecules, but they all work on the principle of sedimentation.
It is driven by an electric motor where the tubes are rotated about a fixed axis, resulting in force perpendicular to the tubes.
A benchtop centrifuge has a rotor with racks for the sample tubes and a lid that closes the working unit of the centrifuge.

Specification:

Capacity 1.6L, maximum speed 15,000 to 15,999 rpm, maximum RCF 5,580 x g, Temperature Range -10°C to +40°C, run time 9 hr. 99 min. plus HOLD

Model: NU 430-400

Brand: Neuere

Biological Safety Cabinet

Status: Available

BSCs offer three levels of protection:

1. Personnel- HEPA filters and an air curtain shield users from bio-hazardous aerosols produced inside the chamber.
2. Sample Protection- Recirculating and unidirectional HEPA-filtered air guards against contamination of samples by contaminated lab air.
3. Lab/ Environmental Protection- HEPA-filtered exhaust from the top of the cabinet safeguards against contaminating the lab environment with biohazardous aerosols inside the chamber.

Working mechanism of Class II Type B2 Biosafety Cabinet

The Type B2 cabinet features an internal motor that pushes air past the supply HEPA filter and into the work zone to produce downflow. The air is drawn into the room through an opening at the top of the cabinet. The air that the internal blower forces down into the work area combines with the air that is drawn into the front access aperture to form the air that is pulled out by the external exhaust blower during normal operation. The only function of the internal motor is to draw air into the opening. The Type B2 cabinet is unable to operate independently. It needs to be paired with an external exhaust blower that has enough force to both pull air into the front of the cabinet and to keep pulling exhaust air out of the cabinet, through the ductwork, and back into the cabinet. The internal blower must stop forcing air down into the work zone if the external exhaust blower ever fails and is no longer able to generate enough “pull” to keep air flowing in that direction toward the user. Hazardous particles could escape from that work zone and move toward the user. There is no recirculation with 100% air exhausted through HEPA filters. These cabinets work best for operations that need the emission of chemical vapors because no air is recirculated in them.

Features of Class II Type B2 Biosafety Cabinet

The front opening
Sash
Exhaust HEPA filter
Supply plenum
Supply HEPA filter
Blower
The negative pressure exhaust plenum
Container Supply Blower
Filter screen

Specification:

Class II Type B2
size nominal 4 foot (1.2m), performance specifications personal protection product protection NSF / ANSI 49
Enclosed containments feature a well-ventilated hood and are intended to safeguard personnel, products, and the environment from hazardous particulates and infectious agents.

Model: Thermo Haake P5 Cycler

Brand: Daigger

Thermo Haake P5 Cycler

Status: Available

Circulating water bath It is also known as a stirrer water bath. In the baths with circulating water, the water is properly circulating. Stirrers or circulating water baths may be useful for applications where the samples must have consistent or uniform temperatures. The circulating water bath uses continuously circulating water to efficiently and dependably obtain and maintain the desired water temperatures required for the cooling or heating of samples and reagents. It enables quick heating or cooling of samples across a wide temperature range. Enzymatic and serologic testing may be used for these purposes. It receives proper circulation, which leads to a more stable temperature.

Specification:

Temperature range = 25-100 ℃, temperature stability = 0.01-0.04 ℃, bath volume = 5L, heater capacity = 1.5kw

Model: BE 400

Brand: Memmert

Incubator Memmert

Status: Available

An incubator is a device that is used in laboratories for the growth and maintenance of microorganisms and cultures. Incubators provide an optimal temperature, pressure, and moisture, among other things required for the growth of microorganisms.

Working Principle

The incubator is based on the principle of maintaining a proper atmosphere for the growth of microorganisms.
Incubators have a heating system that allows for the temperature within the incubator to be adjusted according to the type of organism cultivated inside.
Similarly, they are provided with adjustments for maintaining the concentration of CO2 to balance the pH and humidity required for the growth of the organisms.
Variation of the incubator like a shaking incubator is also available, which allows for the continuous movement of the culture required for cell aeration and solubility studies.

Uses

Incubators have a wide range of applications including cell culture, pharmaceutical studies, hematological studies, and biochemical studies.
Incubators can also be used in the stem cell research area.

Specification:

Temperature range: up to 70 °C

Model: KS 4000 i control

Brand: IKA

Incubator Shaker & Access KS 4000

Status: Available

Incubator shakers are used for both incubation and shaking of samples. Typical applications that use them are cell culture, fermentation, hybridization, biochemistry, enzyme, and cell tissue research. Various temperature characteristics are found in different incubator shakers. Some can be used for both cooling and heating, while others can only raise the temperature above the ambient temperature.


An agitation or shaking process is required to integrate oxygen and distribute nutrients uniformly throughout the culture media in order to create the best conditions for cell growth. Incubator shakers, which combine those activities for a more practical and effective setup, are sometimes used to accomplish that by inserting a separate shaker within an incubator. Any sort of cell growth, including bacterial cultures, tissue cultures, and yeast, can be accomplished with the incubator shaker.

Specification:

Speed Range 10 to 500 rpm
Temperature Range (Metric) Ambient plus 5°C to 80°C
Humidity Range 80%
Timer Range 0 min. to 999 hr.

Model: CK 30

Brand: Olympus

Inverted Microscope

Status: Available

An inverted Microscope is a type of light microscope whose objective lenses and turret are below the stage, and the illuminator and condenser are above the stage. In this type, we have to look upward to see the specimen ideal for tissue culture.
Its working principle and instrumentation are similar to a simple / compound / upright microscope, but the position of the light source, turret, and objective lenses are inverted. In some digitalized types, the camera is fixed to take photos or videos of specimens..

Specification:

Head: Binocular, Eyepieces: 10x Eyepieces, Nosepiece: 4 Position, Objectives: A 4x, A 10x PL Phase, LWD C A 20x PL Phase, Stage: Flat Top Stage, Condenser: N.A. 0.3 w/ CK40-SLP Phase Slider, Focusing: Coarse & Fine, Light Source: Halogen

Model: SSM1

Brand: Stuart

Mini Orbital Shaker

Status: Available

An orbital shaker has a circular shaking motion with a slow speed (25-500 rpm). It is suitable for culturing microbes, washing blots, and general mixing.

Specification:

provides a smooth orbital shaking action with an orbit of 16 mm, ideal for samples of 0.5 to 5 ml held in multi-well plates, dishes, and petri dishes. It features a variable shaking speed from 30 up to 300 RPM and once set, the speed is maintained effectively, even over a long period. Using the digital timer, the shaking run time can be set from 1 second up to 9 hours, or it can be set for continuous operation.

Model: Heracell 150i

Brand: Thermo Scientific

Water Jacketed CO2 Incubator

Status: Available

CO2 incubators are a special kind of incubators that are provided with automatic control of CO2 and humidity. CO2 incubators are used to produce biological cell cultures. They must preserve the same conditions as those found inside the human body i.e. 98.6°F (37°C), > 90% relative humidity & neutral pH 7.4.

Specification:

External (w x h x d) = 470 x 607 x 530mm, Internal (w x h x d) = 470 x 607 x 530mm, Shelves full width (w x d) = 423 x 465mm, No. of shelves (standard/maximum) = 3/10, Max. load per shelf/total load kg = 10/30, Temperature control range = Ambient +3 to 55°C, Constant humidity 3.95 ± 3%rH Fill amount/water quality max. = 3L distilled/autoclaved and demineralized, CO2 Measure and control range Vol = 0 to 20 %, Inlet pressure = 12–15 PSIG (0.8–1 bar), O2 sensor type Zirconium oxide

Model: WNB 22

Brand: Memmert

Water Bath

Status: Available

A water bath is a piece of scientific equipment used to maintain a steady temperature for a prolonged time when incubating samples. Ideal for the temperature control of ointments, emulsions, samples, plates, and nutrient solutions in the laboratory, as well as warm storage. The combination of corrosion-resistant stainless steel, precise electronics and multiple temperature protection guarantees the highest level of safety in the laboratory.
The sensor converts the temperature of the water into a resistance value, which is then amplified and compared by an integrated amplifier. This produces the control signal, which effectively regulates the average heating power of the electric heating tube and keeps the water at a constant temperature.
MEMMERT-water baths are electrically heated and electronically controlled. The temperature of the thermostat liquid is continuously controlled by a microprocessor controller with pulse package control. Electronic microprocessor-PID-controller with continuous power matching and autodiagnostic system with fault indicator (see section 12), integral timer for digital program time selection. The temperature is measured using a Pt 100 temperature sensor (4-wire circuit). The accuracy is as follows:
The components of temperature control are controlled by integrated malfunction recognition. The heaters are installed outside - therefore no problems with dirt or lime residue.

Applications:

It can be used for reagent warming, substrate melting, or cell culture incubation.
The water bath is the preferred heat source for heating flammable compounds because it allows some chemical processes to occur at high temperatures.
It improves the solubility of poorly soluble compounds.

Specification:

Temperature range in ℃: min. 5°C above ambient up to +95°C with additional boiling mode (+100°C)

Model: 411L

Brand: Samsung

Upright Refrigerator (2 Door)

Status: Available

A refrigerator, also known as a “fridge,” is an appliance made up of a thermally insulated compartment and a mechanical, electronic, or chemical heat pump that transfers heat from the interior to the exterior to cool the interior down below room temperature.The fridge in the lab is the same as the one in our house. Its use and calibration needs are what differentiate it. Samples and specimens are cooled in laboratories so they can be preserved. They comprise refrigeration units for holding vaccines and other pharmaceutical or medical supplies, as well as blood plasma and other blood products. To reduce the risk of bacterial contamination and volatile material explosions, laboratory refrigerators must maintain a constant temperature. The refrigerator requires air circulation and a fan to maintain a consistent temperature to operate with high levels of accuracy. To stop cold air from blowing outside the unit when the door is open, the fan shuts off. Laboratory refrigerators have distinct sections to prevent contamination.

Specification:

Capacity = 321 ℓ, Twin Cooling Plus, No Frost, Deodorizer, Number of Shelf (Total) = 3,

Model:

Brand: Masflow

Fume Hood General Purpose

Status: Available

Fume hood represent the primary line of defense for personnel against exposure to harmful, toxic and dangerous fumes or chemicals as they are produced. A fume hood is an enclosure that safely contains and ventilates hazardous fumes, vapors, gasses and dust generated by chemical processes performed in the fume hood. Sometimes called a chemical hood or a lab hood, a fume hood protects workers from inhalation of hazardous substances. The clear sliding window on a fume hood, called the sash, also shields workers from spills and splashes that may occur in the chemical fume hood. Fume hoods are the workhorse of laboratory exhaust systems and are the most widely used approach for local ventilation.