Institut Biologi Sistem

 Institute of Systems Biology

CODA – Metabolomics

Metabolomics is a powerful approach that can provide diagnostic patterns via fingerprinting, absolute quantification of targeted metabolites via pool analysis and relative quantification of large portions of the metabolome using metabolite profiling. Each of these outputs is supported by the two most commonly used techniques, currently available at Chemical Analysis Laboratory, INBIOSIS; gas chromatography mass spectrometer (GC-MS) and liquid chromatography coupled to time of flight (ToF) mass spectrometer (LC-QToF-MS). 

Mass spectrometry (MS) based metabolomics techniques are the most sensitive for simultaneous analysis of a large number of compounds/metabolites. However, researchers often encounter challenging downstream data analysis as the generated raw file formats can be diverse between service providers and MS instruments (either from Bruker, Thermo, Sciex, Agilent etc.) with a plethora of metabolomics analysis software to choose from. CODA-Metabolomics undertakes this endeavour by streamlining proven and meticulous data analysis workflow to provide statistical power to eliminate systematic bias, confidently identify compounds and explore significant findings.  

GC-MS BASED METABOLOMICS AND DATA ANALYSIS WORKFLOW 

METABOLOMICS WORKFLOW (LC-MS)

COMPOUND IDENTIFICATION

The compound identification is based on available library Massbank. The compound spectrum list report must provide data generate from AutoMSn that include the fragmentations. M/z value from the LCMS data will be use in the search engine (MetFrag/Massbank/Metlin). Top 2 result will be listed in the final report.

ADVANCED DATA ANALYSIS

WHAT DO WE OFFER?

Overview of services

Data analysis and bioinformatics⮚     Software packages:
GC-MS
·         MS-DIAL, Met-IDEA, SpeedConnects, AMDIS for data alignment
·         MetaboAnalyst, SPSS for statistical analysis
·         SIMCA P+  for multivariate analysis
LC-TOF-MS
·        Compound identification from available database, Smartformula
·         Data pre-processing service using profile analysis
·         MetaboAnalyst, SPSS for statistical analysis
·         SIMCA P+ for multivariate analysis
·         Pathway mapping and biological interpretation

PRICING DETAILS

TYPE OF DATA ANALYSISCHARGES
GC-MS data analysis
Data analysis including deconvolution and alignment 

Multivariate analysis (PCA, PLS-DA, OPLS-DA) using Simca P+
RM 200 / sample raw data*

RM 200 per hour
LC-MS data analysis
Multivariate analysis

For identification of 100 *compounds depending on complexity

Profile analysis for pre-processing data from raw data
#The raw data must be in .d format

For advanced analysis (biological interpretation/pathway mapping) please contact PIC for more details. Price might varies depending on complexity of data

RM 200 / jam


RM 2,000 –
 

RM 200/jam
 
 
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ACHIEVEMENTS

PERSON IN CHARGE

ChM. Dr. Kamalrul Azlan Azizan

Head of Quality Assurance / Research Fellow

+603 8921 4572

kjkinbio@ukm.edu.my / kamalrulazlan@ukm.edu.my

Metabolomics

Ts. Rafidah Ahmad

Senior Research Officer / Metabolomic lab PIC

+603 8921 4566

fida.ahmad@ukm.edu.my

ChM. Hazim Syahmi Elias

Science Officer / Metabolomic Lab PIC

+603 8921 4566

syahmielias@ukm.edu.my