Workshop Outline
This workshop will focus on the introduction to proteomics analysis through theory and hands-on.
Who should attend
Postgraduate students (Master and PhD students), Post Doctorate, Academicians and Researchers (Government and non-government institution)
List of Speakers and Trainers
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Associate. Prof. Dr. Maizom Hassan obtained her Bachelor (1999), Masters (2004) and Ph.D. Degrees (2008) in Bioresources Science from the University of Tottori, Japan. After completing her studies, she was appointed as a Research Fellow/Senior Lecturer at the Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM). Her current research focuses on the development of biorational insecticide for oil palm pest management by using integrated systems biology, enzymology and entomology approaches. Her other research interests are purification and characterization of several microbial and plant enzymes. Her research interests were manifested in 2 patents, 1 book, 1 book-chapter and 29 publications which include 22 publications in high-impact journals such as Plants, Plant Physiology and Biochemistry, Insects, Phytochemistry, Genes and PloS ONE. Her publications attracted 153 citations with h-index of 8. Expressions of interest in joining the research collaboration and activity with Associate. Prof. Dr. Maizom’s lab are always welcome. Please contact her via e-mail (maizom@ukm.edu.my) for further discussion.
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Dr. Hamizah Shahirah Hamezah's research group has a great interest in understanding the mechanisms of learning and memory, as well as interventions for the cognitive decline during aging and neurodegenerative diseases. They focus on finding possible biomarkers leading to the cognitive decline in brain aging and neurodegenerative diseases by using the state-of-art omics approaches, brain imaging, and cognitive neurosciences. They are interested in finding possible therapeutics for the treatment of neurodegenerative diseases by investigating the effect of natural compounds on transgenic animal models. If you are interested in collaborating with her, please email her at hamizahshahirah@ukm.edu.my.
Programme
8th October 2024 (Tuesday)
Time | Activity | Speaker | Venue |
8.30 am | Registration | Foyer | |
9.00 am | Introduction | Assoc. Prof. Dr. Maizom Hassan | Seminar Hall |
9.05 am | Lecture 1: Introduction to Proteomics - Part 1 ● What is proteomics? ● Gel-based & non gel-based proteomics |
Assoc. Prof. Dr. Maizom Hassan | Seminar Hall |
10.30 am | Morning break | Foyer | |
11.00 am | Lecture 2: Introduction to Proteomics - Part 2 ● Protein extraction ● Protein measurement ● Protein visualization |
Assoc. Prof. Dr. Maizom Hassan | Seminar Hall |
1.00 pm | Lunch break | ||
2.00 pm | Practical 1: ● Protein extraction ● Protein measurement ● SDS-PAGE electrophoresis ● Protein visualization |
●Assoc. Prof. Dr. Maizom Hassan ● Dr. Hamizah Shahirah Hamezah ● AJK kursus latihan |
Proteomics Lab |
5.00 pm | Tea break / END OF DAY 1 | Foyer |
9th October 2024 (Wednesday)
Time | Activity | Speaker | Venue |
9.00 am | Introduction | Dr. Hamizah Shahirah Hamezah | Seminar Hall |
9.30 am | Lecture 3: Introduction to Proteomics - Part 3 ● Mass spectrometry analysis in proteomics |
Dr. Hamizah Shahirah Hamezah | Seminar Hall |
10.30 am | Morning break | ||
11.00 am | Practical 2: ● In-gel digestion tutorial and quality checking |
●Assoc. Prof. Dr. Maizom Hassan ● Dr. Hamizah Shahirah Hamezah ● AJK kursus latihan |
Proteomics Lab |
1.00 pm | Lunch break | ||
2.00 pm | Practical 3: ● LC-MS-TOF demonstration |
● Dr. Hamizah Shahirah Hamezah ● AJK kursus latihan |
Chemical Analysis Workshop |
3.00 pm | Lecture 4: Introduction to Proteomics-Part 4 ● Understanding mass spectra |
Dr. Hamizah Shahirah Hamezah | Seminar Hall |
5.00 pm | Tea-time & end of session | Foyer |
10th October 2024 (Thursday)
Time | Activity | Speaker | Venue |
9.00 am | Introduction | Dr. Hamizah Shahirah Hamezah | Seminar Hall |
9.05 am | Lecture 5: ● Introduction to MaxQuant for proteomics data analysis |
Dr. Hamizah Shahirah Hamezah | Seminar Hall |
10.30 am | Morning break | ||
11.00 am | Practical 4: ● MaxQuant for proteomics data analysis |
● Dr. Hamizah Shahirah Hamezah ● AJK kursus latihan |
Seminar Hall |
1.00 pm | Lunch | ||
2.00 pm | Lecture 6: ● Introduction to Perseus for proteomics data analysis |
Dr. Hamizah Shahirah Hamezah | Seminar Hall |
3.00 pm | Practical 5: ● Perseus for statistical analysis of proteomics data |
Dr. Hamizah Shahirah Hamezah | Seminar Hall |
4.30 pm | Closing ceremony | ●Assoc. Prof. Dr. Maizom Hassan ● Dr. Hamizah Shahirah Hamezah ● AJK kursus latihan |
Seminar Hall |
5.00 pm | Tea time / End of workshop | Foyer |