Institut Biologi Sistem

 Institute of Systems Biology

Workshop Outline

This workshop is aimed at giving participants a detailed understanding of real-time quantitative PCR: 

  • qPCR primer design and experimental considerations

  • Considerations in conducting standard curve and gene expression experiment

  • Hands-on analysis of qPCR data

  • Different applications of qPCR

Who should attend?

​This workshop is open to local & international participants, from beginner to advanced researchers in the field.

Speaker Biography

  • Associate Professor Dr. Goh Hoe Han is a plant molecular biologist who obtained his PhD from the University of Sheffield, United Kingdom in 2011 before starting his first acedemic position at the Institute of Systems Biology, Universiti Kebangsaan Malaysia. Upon joining INBIOSIS, he pioneered the Plant Functional Genomics Research Group focusing on the molecular exploration of tropical plants and crop improvement using functional genomics approaches. Some of the tropical plant species include kesum, ketum, Rafflesia, and Nepenthes pitcher plants, as well as important crops, such as mangosteen, papaya, rice, tomato, and oil palm. Fundamental understandings on the molecular aspects of these plants are crucial for their bioprospecting, industrial application, and crop improvement.

  • Dr Goh is keen on industrial collaboration in precision biotechnology for tropical plant improvement and also the commercialisation of novel recombinant plant enzymes. If you are interested to collaborate or be part of the plant functional genomics group, please email to gohhh@ukm.edu.my.

Programme

16th July 2024 (Tuesday)

Time Activity Venue
09.00 am Registration
9.30 am LECTURE 1 Meeting Room / Seminar Hall
11.00 am Hands-on session:

  • Sample Preparation for Extraction and Lysis (Biotek Abadi)
  • Primer design (Biotek Abadi)
Genomics Lab
12.30 pm Lunch break  
2.00 pm Hands-on session:

  • DNA/RNA Extraction (Biotek Abadi)
  • Spectrophotometric Analysis (Biotek Abadi)
Genomics Lab
4.00 pm Discussion of Extraction Result Genomics Lab
4.30 pm End of workshop

 

17th July 2024 (Wednesday)

Time Activity Venue
09.00 am Registration
9.30 am LECTURE 2 Meeting Room / Seminar Hall
11.00 am Hands-on session:

  • cDNA synthesis
  • Preparation of qPCR mixes
Genomics Lab
01.00 pm Lunch break (Run qPCR)  
2.30 pm Hands-on session: 

  • Data analysis (AP. Dr. Goh Hoe Han, INBIOSIS)
Genomics Lab
4.30 pm Discussion Genomics Lab
5.00 pm End of workshop

Register now or contact us for more details