Workshop Outline
Liquid Chromatography – High Resolution Mass Spectroscopy (LC-HRMS) continued to grow in the last two decades as an analytical technique used in metabolomics. Additionally, by combining spectral resolution, mass accuracy, and mass fragmentation, MS metabolite identification capabilities have been enhanced and recognized as the most employed technique in metabolomics.
We will cover sample preparation (extraction with hands-on), data acquisition (qualitative and quantitative analysis), and finally data analysis using univariate and multivariate approaches in this three-day workshop.
Who should attend
Those who are interested in metabolomics and its applications (research scientists, academicians, postdoctoral fellows, and students) are encouraged to attend the workshop.
List of Speakers and Trainers
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As a pioneer in Metabolomics field in Malaysia, Associate Professor Dr. Syarul Nataqain Baharum research is focused on the new insight of analytical and biological perspectives of the metabolomics in the field of systems biology, in particularly on the understanding of secondary metabolite production in local herbal plants as well as fluxomics studies. She has expanding her research focus in understanding host-pathogen interaction in aquaculture disease and also green therapeutic mechanism in neurogenerative diseases. In metabolomics research, pipeline of analytical tools such as LCMS, GCMS, CEMS, FTIR and NMR combined with Principal Component Analysis (PCA) is essential for the dynamic high throughput analysis. This frontier analysis which includes mathematical modelling has provided glimpse of cellular biology and fingerprint of functional network in the model organism in her lab. Currently she has published about 100 research articles, 4 books and 6 chapters in books. As project leader, she also received more than RM 4 million research grants in total amount. Under her supervision, 9 PhD students and 17 MSc were graduated. At the moment, she is supervising a total of 21 PhD students and 18 MSc students. She also has filed two patents and granted one Trade Mark (TM) and one Trade Secret. Her work has been awarded prestigious awards including BioInnovation Awards in 2011 and Selangor My Innovation Award, 2014. She also engaged in collaborative partnership with industries such as Medika Natura Sdn Bhd, AAR Sdn Bhd and FGV Sdn Bhd.
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If you are interested to collaborate with her, or be part of her research group, please drop her an email to nataqain@ukm.edu.my.
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Dr. Nurkhalida Kamal completed her PhD in Pharmaceutical Sciences (Natural Products Chemistry & Metabolomics) from the University of Strathclyde, Glasgow, UK in 2015. She then worked as a Postdoctoral Fellow at the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) focusing on metabolomics analysis of marine organisms including seaweed and marine microbes. She joined INBIOSIS in 2020 as a Research Fellow.
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Her research focuses on the application of metabolomics in natural products and food chemistry. She wants to understand the chemistry of various resources, why it is produced and what we can use it for. She has vast experiences in natural products isolation and elucidation from various organisms (plants, microbes, marine organisms and mushrooms), NMR and LCMS and chemometric data analysis. Her on-going research is to unravel the mechanism of immunomodulation of polyphenols-rich tempeh fraction on Caco-2 and HT-29 cell lines. Her interest also includes on using metabolomics as a tool to identify and biotechnologically optimize the production of bioactive secondary metabolites in medicinal plants, mushrooms, and microorganisms.
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Please feel free to visit her research group website (www.nurkhalidakamal.com) or contact Dr. Nurkhalida Kamal (nurkhalida.kamal@ukm.edu.my) for research and industrial projects collaboration.
Programme
8 March 2022 (Tuesday)
| Time | Activity | Venue |
| 8.30 am | Registration | Foyer |
| 9.00 am | Opening ceremony | Seminar Hall |
| 9.10 am | Lecture 1: Lecture on metabolites profiling-from extraction to data analysis | Seminar Hall |
| 10.30 am | Morning break | Foyer |
| 11.00 am | Lecture 2: Experimental design of Metabolomics | Seminar Hall |
| 12.30 pm | Lunch break | Foyer |
| 2.00 pm | Practical 1: Hands on sample preparation | Metabolomics Lab |
| 3.00 pm | Afternoon break | Foyer |
| 3.15 pm | Hands on sample preparation | Metabolomics Lab |
| 5.00 pm | End of day 1 |
9 March 2022 (Wednesday)
| Time | Activity | Venue |
| 9.00 am | Introduction to day 2 | Seminar Hall |
| 9.10 am | Lecture 3: Analytical strategies in metabolomics | Seminar Hall |
| 10.30 am | Morning break | Foyer |
| 11.00 am | Practical 4: Hands-on sample preparation for LC-TOF-MS | Chemical Analysis Lab |
| 12.30 pm | Lunch break | Foyer |
| 2.30 pm | Practical 5: Hands-on sample preparation for LC-TOF-MS | Chemical Analysis Lab |
| 3.30 pm | Afternoon break | Foyer |
| 3.45 pm | Practical 6: Sample analysis | Computer Lab |
| 5.00 pm | End of day 2 |
10 March 2022 (Thursday)
| Time | Activity | Venue |
| 9.00 am | Introduction to day 3 | Seminar Hall |
| 9.10 am | Lecture 4: Introduction to Metabolomics Data Analysis | Seminar Hall |
| 10.30 am | Morning break | Foyer |
| 10.45 am | Lecture 5: Introduction to Data Analysis I | Seminar Hall |
| 1.00 pm | Lunch break | Foyer |
| 2.00 pm | Lecture 6: Introduction to Data Analysis II | Seminar Hall |
| 3.30 pm | Afternoon break | Foyer |
| 3.45 pm | Practical 7: Hands-on Introduction to Data Analysis | Seminar Hall |
| 5.00 pm | End of day 3 |