Sains Malaysiana 40(4)(2011): 369–372

Effects of Pink Guava (Psidium guajava) Puree Supplementation on Antioxidant Enzyme Activities and Organ Functions of Spontaneous Hypertensive Rat

(Kesan Suplementasi Puri Jambu Batu Merah (Psidium guajava) ke Atas Aktiviti

Enzim Antioksidan dan Fungsi Organ Tikus Hipertensi Spontan)

Norazmir Md Nor*

Department of Nutrition & Dietetics, Faculty of Health Sciences

Universiti Teknologi MARA, Puncak Alam Campus 42300 Puncak Alam, Selangor D.E., Malaysia

Ayub Mohd Yatim

Deceased

Received: 7 January 2010 / Accepted: 21 June 2010

ABSTRACT

This study was aimed to determine the effects of pink guava (Psidium guajava) puree supplementation on enzyme activities, kidney and liver function tests of Spontaneous Hypertensive Rats (SHR). Twenty-four male SHR were divided into four groups (control, CG (distilled water); low dosage group, LDG (0.5 g/kg body weight); medium dosage group, MDG (1.0 g/kg body weight); high dosage group, HDG (2.0g/kg body weight)). The rats were given pink guava puree via force-feeding and fed rat pellets ad libitum for 28 days in individual cages at 25±2°C. At the end of experiment, the rats were fast overnight (12 to14 h) and euthanized under an anesthetic condition with ether, and blood was collected from the portal vein or posterior vena cava. The specific activities of glutathione peroxidase (GPx) was significantly higher in LDG (2332.5±81.8 U/L), MDG (2424.8±97.1 U/L) and HDG (2594.6±82.8 U/L) respectively, as compared to CG (2171.8±65.9 U/l). Significant differences were also seen in glutathione reductase (GR) activities among all treated groups (LDG (132.5±11.8 U/L), MDG (141.5±16.4 U/L), HDG (148.8±13.2 U/L) compared to CG (126.1±14.2 U/L)). Liver function tests for total antioxidant status (TAS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and γ-glutamyl transpeptidase (GGT) showed significant differences in the treated group compared to control group. In conclusion, this study shows pink guava puree supplementation increase antioxidant enzyme activity in SHR’s blood concentration.

Keywords: Antioxidant; enzyme activity; organ functions; pink guava; spontaneous hypertensive rats

ABSTRAK

Kajian dijalankan untuk menentukan kesan pemberian suplemen puri jambu batu merah (Psidium guajava) ke atas aktiviti enzim, dan ujian kefungsian ginjal dan hati tikus teraruh hipertensi (SHR). Sebanyak 24 ekor SHR dibahagi kepada 4 kumpulan (kawalan, CG (air suling); dos rendah, LDG (0.5 g/kg berat badan); dos sederhana, MDG (1.0 g/kg berat badan); dos tinggi, HDG (2.0 g/kg berat badan)). Tikus diberi puri jambu batu merah melalui suap-paksa dan pelet tikus secara ad libitum selama 28 hari dan diletakkan dalam sangkar individu pada suhu 25±2°C. Akhir kajian, tikus dipuasa semalaman (12-14 jam) dan dibiuskan di bawah pengaruh eter, dan darah diambil dari vena kava posterior. Aktiviti spesifik bagi glutation peroksida (GPx) adalah tinggi secara signifikan dalam LDG (2332.5±81.8 U/L), MDG (2424.8±97.1 U/L) dan HDG (2594.6±82.8 U/L) masing-masing, berbanding CG (2171.8±65.9 U/L). Terdapat perbezaan signifikan pada aktiviti glutation reduktase (GR) dalam kumpulan rawatan (LDG (132.5±11.8 U/L), MDG (141.5±16.4 U/L), HDG (148.8±13.2 U/L) berbanding CG (126.1±14.2 U/L)). Ujian fungsi hati bagi status antioksidan total (TAS), alanin aminotransferase (ALT), aspartat aminotransferase (AST), laktat dehidrogenase (LDH) dan γ-glutamil transpeptidase (GGT) menunjukkan perbezaan signifikan dalam kumpulan rawatan berbanding kumpulan kawalan. Kajian menunjukkan suplementasi puri jambu batu merah meningkatkan aktiviti enzim antioksidan dalam darah tikus hipertensi spontan.

Kata kunci: Antioksidan; aktiviti enzim; fungsi organ; jambu batu merah; tikus hipertensi spontan

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*Corresponding author; email: azmir2790@puncakalam.uitm.edu.my