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Sains Malaysiana 41(2)(2012): 149–154
Clonal Diversity and Genetic
Differentiation in Rhizomatous Herb, Iris japonica (Iridaceae)
Populations on Jinyun Mountain, Southwest China
(Kepelbagaian Klon dan Pembezaan Genetik dalam Populasi Tumbuhan Berizom,
Iris japonica (Iridaceae) di Gunung Jinyun, Barat Daya China)
Yong-Jian Wang*
College of
Horticulture & Forestry Sciences, Huazhong Agricultural University
Wuhan 430070
China
Xue-Ping Shi
College of
Horticulture & Forestry Sciences, Huazhong Agricultural University
Wuhan 430070
China
Zhang-Cheng Zhong
Key Laboratory of
Eco-environments of Three Gorges Reservoir Region
(Ministry of Education) of
Southwest University
Chongqing 400715 China
Received: 31 May
2011 / Accepted: 1 August 2011
ABSTRACT
Many plants can reproduce
both clonally and sexually, and the relative importance between the two modes
of reproduction varied among habitats. Clonal diversity and genetic differentiation of the rhizomatous herb, Iris
japonica populations, was analyzed by 12 randomly amplified polymorphic DNA markers
to determine the extent that reproductive mode varied locally between two
habitats, open area of forest edge (OAFE, 2 populations OAFE 1 and OAFE 2) and bamboo forest (BF,
2 populations BF 1 and BF 2) on Jinyun Mountain, southwest China. Total clonal diversity and
genetic diversity of I. japonica populations were high. The clonal diversity and genetic diversity of I. japonica populations
in BF habitats were lower than those in OAFE habitats.
Neighbor-joining tree by Jaccard’s genetic distance
showed two genetically distinct groups: OAFE group and BF group. AMOVA indicated that about half of the total variation existed within
the four populations, and that genetic differentiation
among habitats (BF populations and OAFE populations)
was also significant. OAFE habitats were helpful in
maintaining clonal diversity and genetic diversity of I. japonica populations than BF habitats. Habitat
differences might have distinguished effects on the relative significance of clonal propagation and sexual reproduction, and further
have a fatal influence on clonal diversity, genetic
diversity and genetic differentiation of I. japonica populations in Jinyun Mountain, Southwest China.
Keywords: Clonal diversity; genetic diversity; Iris
japonica; population differentiation; RAPD
ABSTRAK
Banyak tumbuhan boleh membiak secara klon dan seksual, dan kepentingan relatif di antara kedua-dua cara pembiakan berbeza-beza mengikut habitat. Kepelbagaian klon dan pembezaan genetik bagi populasi tumbuhan berizom, Iris japonica, dianalisis dengan 12 penanda polimorfik DNA teramplikasi rawak untuk menentukan perbezaan setempat cara pembiakan antara dua habitat: kawasan terbuka pinggir hutan (OAFE;
2 populasi – OAFE 1 dan OAFE 2) dan hutan buluh (BF; 2 populasi – BF 1 dan BF 2) di Gunung Jinyun, barat daya China. Kepelbagaian total klon dan genetik bagi populasiI. japonica adalah tinggi. Kepelbagian klon dan genetik bagi populasi BF adalah lebih rendah berbanding populasi OAFE. Pohon Neighbor-joining berdasarkan jarak genetik Jaccard menunjukkan kewujudan dua kumpulan genetik yang berbeza: kumpulan OAFE dan kumpulan BF. AMOVA menunjukkan separuh daripada variasi terkandung dalam empat populasi, dan pembezaan genetik antara habitat (populasi BF dan populasi OAFE) adalah signifikan. Habitat OAFE lebih berguna dalam pengekalan kepelbagaian klon dan genetik populasi I. japonica jika dibandingkan dengan habitat BF. Perbezaan habitat mungkin mempunyai kesan membezakan yang relatif signifikan pada propagasi klon dan pembiakan seksual, dan ini seterusnya memberikan kesan kemandirian pada kepelbagian klon, kepelbagaian genetik dan pembezaan genetik I. japonica di Gunung Jinyun, barat daya China.
Kata kunci: Iris
japonica; kepelbagaian genetik; kepelbagaian klon; pembezaan populasi; RAPD
REFERENCES
Ellstrand,
N.C. & Roose, M.L. 1987. Patterns of
Genotypic Diversity in Clonal Plant Species, American
Journal of Botany 74: 123-131.
Honjo,
M., Ueno, S., Tsumura, Y., Washitani, I. & Ohsawa, R. 2004. Phylogeographic study based on intraspecific sequence variation of
chloroplast DNA for the conservation of genetic diversity in the Japanese
endangered species Primula sieboldii. Biological Conservation 120: 211-220.
Jacquemyn,
H., Brys, R., Honnay, O., Hermy, M. & Rolda´n-Ruiz, I.
2006. Sexual reproduction, clonal diversity and genetic
differentiation in patchily distributed populations of the temperate forest
herb Paris quadrifolia(Trilliaceae). Oecologia 147: 434-444.
Kanno,
H. & Seiwa, K. 2004. Sexual vs. vegetative reproduction in relation to forest dynamics
in the understorey shrub, Hydrangea paniculata(Saxifragaceae). Plant Ecology 170: 43-53
Mandujano,
M.C., Montaña, C., Méndez,
I. & Golubov, J. 1998. The relative contributions of sexual reproduction and clonal propagation in Opuntia rastrerafrom two habitats in the Chihuahuan Desert. Journal of Ecology 86:
911-921.
Nishitani,
S., Takada, T. & Kachi, N. 1999. Optimal resource allocation to seeds and vegetative propagules under density-dependent regulation in Syneilesis palmata(Compositae). Plant Ecology 141: 179-189.
Peakall,
R. & Smouse, P.E. 2006. GENALEX 6:
genetic analysis in Excel. Population genetic software for
teaching and research. Molecular Ecology Notes 6: 288-295.
Pielou, E.C. 1969. An Introduction to Mathematical Ecology. New York:
Wiley-Interscience.
Pollux,
B.J.A., Jong, M.D.E., Steegh,
A., Verbruggen, E., Van Groenendael,
J.M. & Ouborg, N.J. 2007. Reproductive strategy, clonal structure
and genetic diversity in populations of the aquatic macrophyte Sparganium emersum in river systems. Molecular Ecology 16: 313-325.
Prati,
D. & Schmid, B. 2000. Genetic differentiation of life-history traits within populations
of the clonal plant Ranunculus reptans. Oikos 90: 442-456.
Ruan,
C.J., He, Z.X. & Zhou, C.F. 2005. Plant Molecular Ecology.
Beijing: Chemical Industry Press.
Rusterholz,
H.P., Kissling, M. & Baur,
B. 2009. Disturbances by human trampling alter the performance, sexual reproduction and
genetic diversity in a clonal woodland herb. Perspectives
in Plant Ecology, Evolution and Systematics11:
17-29.
Tarasjev,
A. 2005. Impact of genet size and flowering stage on fruit set in Iris pumilaL. clones in wild. Acta Oecologica27: 93-98.
Tucic, B., Milojkovic, S., Vujcic, S. & Tarasjev, A. 1988. Clonal diversity and dispersion in Iris pumila. Acta Oecologica9:
211-219.
Wang,
Y.J. & Fang, X. 2010. Adaptability of Iris
japonica Thunb. in different habitats in Three Gorges Reservoir Regions. The Journal of
Chongqing Normal University (Natural Science) 27(5): 28-32.
Wang,
Y.J., Fang, X. & Zhang, Z.C. 2010. Effect of
different habitats on floral, fruit and seed traits of Iris japonica Thunb. Acta Ecologica Sinica30(17):
4628–4635.
Wang,
Y.J., Tao, J.P. & Zhong, Z.C. 2009. Factors
influencing the distribution and growth of dwarf bamboo, Fargesia nitida, in a subalpine forest in Wolong Nature Reserve, southwest China. Ecological
Research 24: 1013-1021.
Wang,
Y.J., Zhong, Z.C. & Tao, J.P. 2008. Patterns of ramet population of Iris
japonica Thunb. and their effects on herb diversity in different microsites on Jinyun Mountain, China. Acta Ecologica Sinica (International Journal) 28(7): 3082-3091.
Wilk,
J.A., Kramer, A.T. & Ashley, M.V. 2009. High variation in clonal vs. sexual
reproduction in populations of the wild strawberry, Fragaria virginiana(Rosaceae). Annals of Botany 104: 1413-1419.
Yamagishi,
M., Nishioka, M. & Kondo, T. 2010. Phenetic diversity in the Fritillaria camschatcensispopulation grown on the Sapporo campus
of Hokkaido University. Landscape and Ecological Engineering 6:
75-79.
Yang,
Y.J., Wu, N.B., Yang, C.X., Chen, M., Tang, Y.M., Tan, F. & Liao, Z.H.
2006. A protocol for extraction of high-quality DNA and RNA from Taxol-producing endophyte. Journal of Southwest Agricultural University (Natural Sciences) 28(3):
33-36.
Yeh,
F.C. & Boyle, T.J.B. 1997. Population genetic
analysis of co-dominant and dominant markers and quantitative traits. Belgian
Journal of Botany 129: 157.
Young,
A.G., Hill, J.H., Murrayc, B.G. & Peakall, R. 2002. Breeding system, genetic
diversity and clonal structure in the subalpine forb Rutidosis leiolepisF. Muell. (Asteraceae). Biological
Conservation 106: 71-78.
Zhang. M. & Huang, S.Z. 2008. RAPD analysis on some germplasm of Iris L. plants. Acta Botanica Boreali-Occidentalia Sinica28(5): 933-939.
*Corresponding author; email:
wangyj@mail.hzau.edu.cn
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