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Sains Malaysiana 44(7)(2015): 947–955 Pure
Cultivation and Morphological Studies of Four Chaetoceros
Taxa from the Coastal Waters of Pahang, Malaysia (Pengkulturan
Tulen dan Kajian Morfologi Empat Takson Chaetoceros
dari Persisiran
Pantai Pahang, Malaysia) M.A.M.
FUAD1*,
N.
MOHAMMAD-NOOR2,
A.K.C.
JALAL2
& B.Y. KAMARUZZAMAN2 1Institut Kesihatan Umum, Kementerian Kesihatan Malaysia,
Jalan Bangsar, 50590 Kuala Lumpur Malaysia 2Kulliyyah of Science,
International Islamic University Malaysia, Jalan
Istana, Bandar Indera Mahkota,
25200 Kuantan, Pahang Darul Makmur, Malaysia Received: 1 December
2013/Accepted: 2 February 2015 ABSTRACT This study was conducted to
study the morphological features of species under the genus Chaetoceros isolated from the coastal waters of
Pahang, Malaysia. The species were isolated, cultivated and viewed
under light microscope (LM) and scanning electron microscope (SEM).
Four taxa were successfully isolated and cultivated in pure culture,
namely Chaetoceros affinis var. affinis, Chaetoceros affinis var. willei, Chaetoceros anastomosans and
Chaetoceros baculites. Both varieties
of C. affinis were considered as
distinct taxa where both strains can be distinguished based on morphological
characteristics. C. affinis var.
affinis has a thick cell wall compared with C. affinis var. willei. C.
anastomosans has special features including
a silica bar on the intersection of setae and the production of
mucous. The length of the aperture opening is a new record from
this study. The new record obtained for C. baculites
includes the size of the aperture, the terminal setae, spinal
arrangement, the thinness of the cell wall
and the location of the species in tropical waters. Detailed data
on each species will be added to the taxonomic information for future
studies. Keywords: Chaetoceros; coastal water; LM;
morphology; SEM ABSTRAK Kajian ini dijalankan untuk
mengkaji ciri morfologi spesies di bawah genus Chaetoceros
yang diasingkan dari pesisir pantai Pahang, Malaysia. Kesemua
spesies dipencil, dikultur dan diperiksa melalui miskroskop cahaya
(LM)
dan mikroskop elektron imbasan (SEM). Empat takson telah berjaya
dipencilkan dan dikulturkan kepada kultur tulen, iaitu Chaetoceros
affinis var. affinis, Chaetoceros affinis var.
willei, Chaetoceros anastomosans, dan Chaetoceros
baculites. Kedua-dua varieti C. affinis dikategorikan
sebagai takson terdekat dengan kedua-dua strain boleh dibezakan
berdasarkan ciri morfologi. C. affinis var. affinis mempunyai
dinding sel yang tebal berbanding C. affinis var. willei.
C. anastomosans mempunyai ciri-ciri istimewa iaitu bar silika
di persilangan seta dan penghasilan mukus. Panjang bukaan merupakan
rekod terbaru di dalam kajian ini. Rekod terbaru diperoleh bagi
C. baculites yang merangkumi saiz bukaan, seta utama, susunan
duri dan kenipisan dinding sel dan spesies ini dijumpai di kawasan
tropika. Keperincian data untuk setiap spesies akan ditambah ke
dalam informasi taksonomi untuk kajian masa hadapan. Kata kunci: Chaetoceros; LM;
morfologi; pesisiran
pantai; SEM REFERENCES Asma, T. & Saifullah, S.M. 2010. The
planktonic diatom of the genus Chaetoceros
Ehrenberg from northwestern Arabian sea bordering Pakistan.
Pakistan Journal of Botany 42(2): 1137-1151. Be´rard-Therriault, L.,
Poulin, M. & Bosse´,
L. 1999. Guide d’identification du phytoplancton marin
de l’estuaire et du golfe
du Saint-Laurent incluant e´ galement
certains protozoaires.
Publication spéciale canadienne
des sciences halieutiques et
aquatiques 128: 1-387. Boonyapiwat, S. 1998. Distribution, abundance and
species composition of phytoplankton in the South China Sea, Area
II: Sabah, Sarawak and Brunei Darussalam. Proceedings of the
Second Technical Seminar on Marine Fishery Resources Survey in the
South China Sea, Area II: West Coast of Sabah, Sarawak and Brunei
Darussalam. Kuala Lumpur, Malaysia. 14-15 December. Cupp, E.E. 1943. Marine planktonic diatoms of the west coast of North
America. Bulletin of the Scripps Institution of Oceanography
5(1): 1-238. Evensen, D.L.
& Hasle, G.R. 1975. The morphology of some Chaetoceros
(Bacillariophyceae) species as seen
in the electron microscopes. Beiheft
Nova Hedwigia 53: 153-184. Gran,
H.H. 1897. Botanik. Protophyta: Diatomaceae,
Silico-flagellata or Cilioflagellata.
Den Norske Nordhavs-Expedition 1876-1878
7(24): 1-36. Grunow, A.
1888. U¨ ber einige neue und ungenu¨ gend bekannte
Arten und Gattungen.
Verh zool-bo. Ges Wien 13: 137-162. Harrison,
P.J. & Berges, J.A. 2005. Chapter 3: Marine Culture
Media. In Algae Culturing Technique, edited
by Anderson, R.A. New York: Elsevier. pp. 21-33. Hasle, G.R. & Syvertsen, E.E. 1997.
Marine diatoms. In Identifying
Marine Phytoplankton, edited by Tomas, C.R. New York:
Academic Press. pp. 5-386. Hendey, N.I. 1964. An introductory account of the smaller algae of British coastal waters.
Fishery Investigations Series 1 V, Part V. Bacillariophyceae (Diatoms). H.M.
Stationery Office. pp. 1-317. Herna´ndez-Becerril,
D.U. 1996.
A morphological study of Chaetoceros
species (Bacillariophyta) from the
plankton of the Pacific Ocean of Mexico. Bulletin of the
Natural History Museum 26: 1-73. Herna´ndez-Becerril,
D.U. & Ake´-Castillo, J.A. 2001. Morphological study of two marine planktonic
diatoms of the genus Chaetoceros:
C. anastomosans and
C. costatus. In Lange-Bertalot-
Festschrift: Studies on Diatoms, edited by Jahn,
R., Kociolek, J.P., Witkowski,
A. & Compe`re, P. Ruggell,
Liechtenstein: Gantner. pp. 53-62. Herna´ndez-Becerril,
D.U. & Granados, F. 1998. Species of the diatom
genus Chaetoceros (Bacillariophyceae)
in the plankton from the southern Gulf of Mexico. Botanica
Marina 41: 505-519. Horner,
R.A. 2002.
A Taxonomic Guide to Some Common Marine Phytoplankton.
Bristol: Biopress Ltd. pp. 1-206. Itakura, S. 2000. Physiological
ecology of the resting stage cells of coastal planktonic diatoms.
Bulletin of Fisheries and Environment of Inland
Sea 2: 67-130 (in Japanese with English abstract). Jensen,
K.G. & Moestrup, Ø. 1998. The genus Chaetoceros (Bacillariophyceae) in inner Danish coastal waters.
Opera Botanica 133: 1-68. Konno,
S., Inoue, N., Hernández-Becerril, D.U.
& Jordan, R.W. 2010.
Chaetoceros affinis
blooms in palauan meromictic marine
lakes. Vie et milieu-life and environment
60(3): 257-264. Lauder,
H.S. 1864.
Remarks on the marine diatomaceae found
at Hong Kong, with description of new species. Transactions of
the Microscopical Society of London, New
Series 12: 75-79. Mohammad-Noor,
N. 2012.
Chapter 13: Scanning Electron Microscope. Basic
Knowledge in Marine Sciences, First Edition. International
Islamic University Malaysia: IIUM Press. pp. 1-100. Rika
Partiwi, A., Dahrul,
S., Linawati, H., Lily Maria, G.P. & Maggy,
T.S. 2009.
Fatty acid synthesis by Indonesian marine diatom,
Chaetoceros gracilis.
HAYATI Journal of Biosciences 16(4): 151-156. Rines, J.E.B. 1999.
Morphology and taxonomy of Chaetoceros
contortus Schutt
1895, with preliminary observations on Chaetoceros
compressus Lauder 1864 (Subgenus Hyalochaete, Section Compressa).
Botanica Marina 42: 539-551.
Rines,
J.E.B. & Theriot, E.C. 2003. Systematics of
Chaetocerotaceae (Bacillariophyceae):
I. A phylogenetic analysis of the family. Phycological
Research 51(2): 83-98. Rines,
J.E.B. & Hargraves, P.E. 1988. The Chaetoceros
Ehrenberg (Bacillariophyceae) flora
of Narragansett Bay, Rhode Island, U.S.A. Bibliotheca Phycologica
79: 1-196. Shevchenko,
O.G., Orlova, T.Y. & Herna´ndez-Becerril,
D.U. 2006.
The genus Chaetoceros
(Bacillariophyta) from Peter the Great Bay, Sea of Japan.
Botanica Marina 49: 236-258.
Simonsen, R. 1974. The diatom plankton of the Indian Ocean Expedition of RV “Meteor”
1964-1965. “Meteor” Forsch.
Ergebnisse 19: 1-107. Sunesen, I., Hernández-Becerril, D.U. & Sar,
E.A. 2008. Marine diatoms from Buenos Aires coastal
waters (Argentina).V. Species of the genus Chaetoceros.
Revista de Biología
Marina y Oceanografía 43(2): 303-326.
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