Sains Malaysiana 44(8)(2015): 1103–1110

Identification of Indigenous Bacteria Isolated from Shrimp Aquaculture Wastewater with Bioremediation Application: Total Ammoniacal Nitrogen (TAN) and Nitrite Removal

(Pengenalpastian Bakteria Asli Dipencilkan daripada Air Sisa Udang Akuakultur dengan Aplikasi BioPemulihan: Jumlah Amonia Nitrogen (TAN) dan Penyingkiran Nitrit)

SARMILA MUTHUKRISHNAN^1,3, VIKINESWARY SABARATNAM^1,2, GEOK-YUAN

ANNIE TAN^1,2,3 & VING-CHING CHONG¹*

¹Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur

Malaysia

²Mushroom Research Centre, Institute Biological Sciences, Faculty of Science, University of Malaya

50603 Kuala Lumpur, Malaysia

³Institute of Ocean and Earth Sciences (IOES), University of Malaya, 50603 Kuala Lumpur

Malaysia

Received: 10 August 2014/Accepted: 24 March 2015

ABSTRACT

Deterioration of water quality mainly due to high total ammonia nitrogen (TAN) and nitrite will affect the productivity of shrimp culture. In this study, three indigenous strains assigned as VCM5, VCM8 and VCM12 were evaluated for their ability to degrade TAN and nitrite. These strains were inoculated into shrimp aquaculture wastewater to enhance the degradation of TAN and nitrite. All the strains reduced TAN and nitrite level from the shrimp aquaculture wastewater significantly (p<0.05). Strain VCM5 (GenBank accession number KJ700465) and VCM8 (GenBank accession number KJ700464) showed 99.71% sequence similarity with the 16S rRNA gene type species Bacillus vietnamensis 15-1T (ABO99708) and strain VCM12 (GenBank accession number KJ700463) showed 99.05% sequence similarity with 16S rRNA gene sequence type species Gordonia bronchialis DSM43247T (CP001802).

Keywords: Bioremediation; shrimp; total ammonia nitrogen

ABSTRAK

Kemerosotan kualiti air terutamanya disebabkan oleh jumlah nitrogen amonia yang tinggi (TAN) dan nitrit akan menjejaskan produktiviti kultur udang. Dalam kajian ini, tiga strain asli dikenali sebagai VCM5, VCM8 dan VCM12 telah dinilai keupayaannya untuk mendegradasi TAN dan nitrit. Strain ini telah diinokulasi ke dalam air sisa udang akuakultur untuk meningkatkan degradasi TAN dan nitrit. Semua strain menurunkan tahap TAN dan nitrit daripada air sisa akuakultur udang dengan ketara (p<0.05). Strain VCM5 (nombor penerimaan GenBank KJ700465) dan VCM8 (nombor penerimaan GenBank KJ700464) menunjukkan 99.71% persamaan jujukan dengan spesies jenis gen 16 rRNA Bacillus vietnamensis 15-1T (ABO99708) dan strain VCM12 (nombor penerimaan GenBank KJ700463) menunjukkan persamaan jujukan 99.05% dengan16 rRNA spesies jenis jujukan gen Gordonia bronchialis DSM43247 T (CP001802).

Kata kunci: Biopemulihan; jumlah nitrogen amonia; udang

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*Corresponding author; email: chong@um.edu.my