Sains Malaysiana 46(12)(2017): 2447–2454

http://dx.doi.org/10.17576/jsm-2017-4612-21

 

A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

(Penderia Baru yang Berasaskan Nano zarah Perak untuk Pengesanan Antigen Virus Ensefalitis Jepun)

 

LIH SHAN LIM, SUK FUN CHIN*, SUH CEM PANG, MAGDLINE SIA HENRY SUM

 & DAVID PERERA

 

Department of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak

94300 Kota Samarahan, Sarawak Bumi Kenyalang, Malaysia

 

Received: 1 September 2016/Accepted: 3 April 2017

 

ABSTRACT

A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optical sensing probes. The detection of JEV antigen was observed via changes in light absorbance by Ag NPs upon occurrence of JEV antigen-antibody bindings. A highly sensitive and rapid optical sensing probe for JEV antigen with a detection limit of 12.8 ng/mL (for S/N ratio = 3) and an analysis assay time of 1 h had been demonstrated.

 

Keywords: Antibody; biosensor; Japanese Encephalitis Virus (JEV) antigen; optical sensing probe; silver nanoparticles (Ag NPs)

ABSTRAK

Biopenderia optik baru yang berasaskan nano zarah perak (Ag NPs) telah dihasilkan untuk pengesanan antigen virus Ensefalitis Jepun (JEV). Ag NPs telah dimendapkan di atas slaid kaca yang difungsikan dengan amino. Seterusnya antibodi JEV dipegunkan pada permukaan Ag NPs untuk membentuk biopenderia optik. Pengesanan terhadap JEV antigen diperhatikan melalui perubahan penyerapan cahaya oleh Ag NPs apabila berlakunya pengikatan antara JEV antigen dan antibodi. Satu biopenderia optik yang sensitif bagi pengesanan JEV dengan had pengesanan (LOD) sebanyak 12.8 ng/mL (ratio S/N = 3) dan 1 jam masa analisis telah dihasilkan.

 

Kata kunci: Antibodi; antigen virus Ensefalitis Jepun (JEV); biopenderia; nano zarah perak (Ag NPs); perangsang optic

 

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*Corresponding author; email: sukfunchin@gmail.com