Sains Malaysiana 50(9)(2021): 2563-2577

http://doi.org/10.17576/jsm-2021-5009-06

Biological Activities and Chemical Profiles of Selected Soil Fungi Isolated from Sirindhorn Peat Swamp Forest of Narathiwat, Thailand

(Aktiviti Biologi dan Profil Kimia Kulat Tanah Terpilih yang Dipencilkan dari Hutan Paya Gambut Sirindhorn di Narathiwat, Thailand)

SUKANYA DEJ-ADISAI*, NARISORN KAEWCHUTIMA, CHATCHAI WATTANAPIROMSAKUL & SATHIANPONG PHOOPHA

Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand

Received: 3 February 2020/Accepted: 6 January 2021

ABSTRACT

A total of 54 soil fungi were collected from Sirindhorn peat swamp forest in Narathiwat Province which is the last peat swamp forests in Thailand. They were extracted using four different solvents and 104 crude extracts were obtained. The bioactivity screening of these samples was done employing alpha-glucosidase inhibition, anti-inflammation using nitric oxide inhibition and cytotoxicity using cancer cell line (MCF-7). The results showed that SPSF224 crude extract had the best biological activities in the screening tests and was then selected for further study. The SPSF224 was identified as Penicillium maximae. The crude extracts of P. maximae were determined for chemical profile, IC₅₀ value of alpha-glucosidase inhibition, antioxidant and antimicrobial activities against Staphylococcus aureus, Trichophyton mentagrophytes, and T. rubrum. This was the first report of chemical investigation and biological activity evaluation of P. maximae.

Keywords: Alpha-glucosidase inhibition; antidiabetic; anti-microbial; antioxidant; Penicillium maximae

ABSTRAK

Sebanyak 54 kulat tanah telah dikumpulkan daripada hutan paya gambut Sirindhorn di Wilayah Narathiwat yang merupakan hutan paya gambut terakhir di Thailand. Kesemuanya diekstrak menggunakan empat pelarut yang berbeza dan 104 ekstrak kasar diperoleh. Penyaringan bioaktiviti sampel ini dilakukan dengan menggunakan aktiviti perencatan alfa-glukosidase, anti-radang menggunakan perencatan nitrik oksida dan sitotoksisita menggunakan titisan sel kanser (MCF-7). Hasil kajian menunjukkan bahawa ekstrak kasar SPSF224 mempunyai aktiviti biologi terbaik dalam ujian saringan dan kemudian dipilih untuk kajian lebih lanjut. SPSF224 dikenal pasti sebagai Penicillium maximae. Ekstrak kasar P. maximae ditentukan untuk profil kimia, nilai IC₅₀ perencatan alfa-glukosidase, aktiviti antioksida dan antimikrob terhadap Staphylococcus aureus, Trichophyton mentagrophytes dan T. rubrum. Ini adalah laporan pertama penyelidikan kimia dan penilaian aktiviti biologi P. maximae.

Kata kunci:      Antidiabetik; antimikrob; antioksidan; Penicillium maximae; perencatan alfa-glukosidase

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*Corresponding author; email: sukanya.d@psu.ac.th