Sains Malaysiana 31: 109-115 (2002) Sains Fizis dan Gunaan /
Physical and Applied Sciences
Penurunan Sikloheksanon kepada Sikloheksanol oleh bakteria penghasil
aseton-butanol, Clostridium acetobutylicum NCIB 8052
(Reduction of cyclohexanone to cyclohexanol by acetone-butanol
producing bacteria, Clostridium acetobutylicum NCIB 8052)
Mohd Sahaid Kalil & Noorhisham Tan Kofli
Jabatan Kejuruteraan Kimia dan Proses
Fakulti Kejuruteraan
Universiti Kebangsaan Malaysia
43600 UKM Bangi, Selangor D.E., Malaysia
ABSTRAK
Penyelidikan ini bertujuan mengkaji tindak balas penurunan sikloheksanon kepada sikloheksanol secara biotransformasi menggunakan Clostridium acetobutylicum NCIB 8052. C. acetobutylicum ditumbuhkan dalam media RCM dalam kultur tiub yang mengandungi sikloheksanon pada kepekatan 130 mM. Kajian biotransformasi dalam fermenter dilakukan menggunakan media minima glukosa yang mengandungi sikloheksanon (5 mM). Hasil biotransformasi dianalisis menggunakan kromatografi gas (GC). Kepekatan sikloheksanol yang terhasil didapati meningkat secara linear dengan peningkatan substrat yang diberikan sehingga 10 mM. Kepekatan substrat yang lebih tinggi secara relatifnya tidak meningkatkan hasil. Kajian ini menunjukkan bahawa biotransformasi sikloheksanon merupakan proses yang tidak berkait dengan pertumbuhan dan penghasilan butanol merupakan prasyarat untuk biotransformasi.
ABSTRACT
The aim of this project was to investigate the reduction of cyclohexanone to cyclohexanol by Clostridium acetobutylicum NCIB 8052. C. acetobutylicum was grown in a tube culture containing RCM medium with added cyclohexanone at concentrations of 1-30 mM. Biotransformation studies in a fermenter was carried out using glucose minimal media containing 5 mM cyclohexanone. Products were ana lysed by gas chromatography (GC). It was found that cyclohexanol concentration increased with an increase in substrate concentration up to 10 mM. However, higher substrate concentration did not increase product concentration. This study showed that biotransformation of cyclohexanone was not growth-linked and the production of butanol was a prerequisite for biotransformation to occur.
RUJUKAN/REFERENCES
Green, E.M., Kalil, M.S., Williams, P. & Stephens, G.M. 1994. Screening for reduction of aldehydes and ketones by solventogenic cultures of the strict anaerobe. Clostridium acetobutylicum. Biotechnology Techniques 8(10): 733-738.
Hastings, J.H.J. 1978. Acetone-butyl alcohol fermentation. In A.H. Rose (ed), Economic Mkicrobiology, vol. 2. Primary products of metabolism. New York: Academic press, Inc., p.31-45.
Jones, D.T., Westruizen, A.V-D., Long, S., Allcock, E.R., Reid, S.J. & Woods, D.R. 1982. Solvent production and morphological changes in C. acetobutylicum. Appl. Env. Microbiol. 43: 1434-1439.
Jones, D.T. & Woods, D.R. 1986. Gene Transfer, Recombination & Gene Cloning in C. acetobutylicum. Microbiol. Sci. 3: 19-22.
Mitsuda, S., Uminura, T. & Hirohara, H., 1988. Preparation of an optically pure secondary alcohol of synthetic pyrethroids using microbial lipases. Appl. Microbiol. Biotechnol. 29: 310-315.
Mohd. Sahaid Kalil, Gillian M. Stephens, Mohd. Ramlan M.S. & Abdul Amir H.K. 1997. Reduction of ketones by solvent-producing cultures of Clostridium acetobutylicum NCIB 8052. 3rd Symposium on Trends in Biotechnology, Universiti Putra Malaysia, Serdang 211-213.
Roberts, S.M. 1989. Production of man-made molecules using natural catalysts. Kemia-Kemi 16: 250-251.
Stephens, G.M., Holt, R.A., Gottschal, J.C. & Morris, J.G. 1985. Studies on the stability of silvent production by Clostridium acetobutylicum in continuous culture. J. Appl. Bacteriol 59: 597-605.
|