Sains Malaysiana 32: 147-155 (2003) Sains Hayat/ Life Science
Ketoksikan Logam Zink dan Kadmium terhadap Anabaena flos-aquae (Toxicity of zinc and cadmium on Anabaena flos-aquae)
Khairiah Jusoh, Carol Hoh Mui Ling & Nik Mazidah Nik Ahmad Pusat Pengajian Sains Ssekitaran dan Sumber Alam Fakulti Sains dan Teknologi Universiti Kebangsaan Malaysia 43600 UKM Bangi, Selangor D.E., Malaysia
Nik Marzuki Sidik Pusat Pengajian BioSains dan Bioteknologi Fakulti Sains dan Teknologi Universiti Kebangsaan Malaysia 43600 UKM Bangi, Selangor D.E., Malaysia
Lee Yook Heng Pusat Pengajian Sains Kimia dan Teknologi Makanan Fakulti Sains dan Teknologi Universiti Kebangsaan Malaysia 43600 UKM Bangi, Selangor D.E., Malaysia
ABSTRAK
Kajian pengambilan logam zink (Zn) dan kadmium (Cd) oleh alga biru-hijau Anabena flos-aquae dan ketoksikan logam-logam ini telah dilakukan secara pengkulturan statik berasingan. A. flos-aquae didedahkan kepada kepekatan Zn dan Cd yang berbeza-beza. Kadar pertumbuhan A. flos-aquae ditentukan dengan kaedah spektrofotometri dan paras ketoksikan LD50 96 jam ditentukan melalui analisis Probit. Hasil kajian menunjukkan bahawa pengambilan kedua-dua logam ini oleh A flos-aquae mengikut corak yang serupa, iaitu apabila kepekatan logam meningkat, kuantiti logam diserap berkurangan. Walau bagaimanapun, pengambilan Zn oleh A. flos-aquae ialah tiga kali lebih tinggi berbanding Cd pada paras pendedahan 1.00 mg/L logam. lni menunjukkan Zn adalah lebih diperlukan oleh A. flos-aquae berbanding Cd. Pengambilan Cd yang rendah juga disebabkan oleh ketoksikan Cd yang lebih toksik. LD50 96 jam untuk Cd ialah 0.14 mg/L berbanding dengan LD50 96 jam Zn iaitu, 0.76 mg/L.
ABSTRACT
The uptake of the heavy metals zink (Zn) and cadmium (Cd) by the blue-green algae Anabena flos-aquae and their toxicity were studied using different static cultures. A. flos-aquae was exposed to different concentrations of Zn and Cd. The growth of the algae was monitored by spectrophotometric assay and the level of toxicity, LD50 96 h was measured by using Probit analysis. The study showed that uptake heavy metals by A. flos-aquae followed a similar trend but Zn was absorbed more than Cd. At the level of exposure of 1.00 mg/L for both metals, the absorption of Zn was three times higher than that of Cd. This indicates that Zn is required by A. flos-aquae as compared to Cd The low level of uptake of Cd may be attributed to the higher toxicity level of Cd LD50 96 h for Cd was 0.14 mg/L compared with that of Zn which was 0.76 mg/L.
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