Sains Malaysiana 33(2): 83-88 (2004)                                                           Sains Fizis dan Gunaan /

                                                                                                                                Physical and Applied Sciences

Pengasingan dan Kajian Struktur Andrografolid daripada

Andrographis paniculata Nees

(Separation and Structural Study of Andrographolide from

Andrographis paniculata Nees)

Wan Zuraida Wan Mohd Zain. Nik Loris Yusoff.

Jalifah Latip & Bohari M. Yamin

Pusat Pengajian Sains Kimia dan Teknologi Makanan

Fakulti Sains dan Teknologi

43600 UKM Bangi, Selangor D.E.  Malaysia

ABSTRAK

Andrografolid telah dipisahkan daripada herba Andrographis paniculata Nees dengan kaedah kromatografi turus dan dihablur semula daripada metanol.  Analisis spektroskopi RMN ¹H dan ¹³C, 1M, UL, KGSJ bersetuju dengan laporan terdahulu. Hablur produk mempunyai sistem monoklinik dengan kumpulan ruang P2₁, a=6.541(1), b=8.003(1), c=I7.962(2)Å dan β =97.39(1)°. Fragmen bisikloheksana berkonformasi kerusi dengan kumpulan metil dan CH₂OH mengisi kedudukan paksi. Kumpulan OH serta C₂-lakton berada di kedudukan khatulistiwa. Panjang dan sudut ikatan adalah normal. Terdapat dua ikatan intrahidrogen O-H .... O dan satu C-H .. O. Molekul distabilkan oleh dua ikatan interhidrogen O-H ……O dan C-H .… O membentuk rangkaian polimer tak terhingga selari dengan paksi- b.

ABSTRACT

Andrographolide was separated from Andrographis paniculata Nees by using column chromatography and recrystallized from methanol. ¹H and ¹³C NMR, IR, UV spectroscopic and GCMS data were in agreement with the previous report. The crystallined product has a monoclinic system with space group P2₁, a=6.541(1), b=8.003(1), c=17.962(2) Å and β=97.39(1)19^o. The bicyclohexane fragments have a chair conformation with the methyl and the methylene hydroxyl groups occupying the axial position, while the OH and the C₂-lactone are in the equatorial positions. The bond lengths and the angles are in the normal ranges. There are two intramolecular interactions of the type O-H … O and C-H …. O in the molecule. The molecule is stabilized by two interhydrogen bonds O-H … O and C-H … O to form polymeric chains parallel to b-axis.

RUJUKAN/REFERENCES

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