Sains
Malaysiana 38(5)(2009): 767–771
Biological Activities of Four Melicope Species
(Aktiviti
Biologi bagi Empat Spesies Melicope)
Najihah Mohd Hashim1, Mawardi Rahmani1*, Hazar Bebe Mohd Ismail2,
Mohd Aspollah Sukari1, A. Manaf Ali3 & Gwendoline Ee Cheng Lian1
1Department of Chemistry, Universiti Putra Malaysia
43400 UPM Serdang, Selangor D.E., Malaysia
2Centre for Foundation Study in Science
Universiti Malaya, 50603 Kuala
Lumpur, Malaysia
3Department of Biotechnology, Universiti Putra Malaysia
43400 UPM Serdang, Selangor D.E., Malaysia
Diserahkan: 13 Jun 2008 / Diterima: 30 Disember
2008
ABSTRACT
The leaves
and stem barks extracts of four plant species, Melicope confusa, M. clemensiae, M. lunu-ankenda and M.
latifolia were screened for their antimicrobial, antioxidant and cytotoxic
activities. Almost all extracts of M. clemensia exhibited moderate
growth inhibition when tested against the three pathogenic fungi. However, when
tested against pathogenic bacteria, the three leaves extracts showed strong
activity against methicillin resistant S. aureus and moderate for the
other bacteria. In the antioxidant assay by using DPPH, the three leaves extracts of M. lunu-ankenda exhibited significant activity with with IC50 values of 7.8, 15.6 and 7.8 μg/mL. In addition, the
petroleum ether leaves extract of M. clemensiae and the chloroform leaves
extract of M. lunu-ankenda showed strong
cytotoxic activity when tested against human promyelocytic leukemia (HL60) cell
line with IC50 values of 2.8 and 0.8 μg/mL, respectively.
Keywords:
Antioxidant antimicrobial; cytotoxic; M.
clemensiae; M. lunu-ankenda; M. latifolia; Melicope-confusa
ABSTRAK
Ekstrak daun dan kulit batang daripada empat spesies tumbuhan, Melicope confusa, M. Clemensiae, M. lunu-ankenda dan M.
latifolia telah dilakukan penabiran aktiviti antimikrob, antioksidan dan
sitotoksik. Hampir
semua ekstrak M. clemensiae menghalang
pertumbuhan secara sederhana bila diuji dengan kulat patogenik. Tetapi
apabila diuji dengan bakteria patogenik, ketiga-tiga ekstrak daun menunjukkan
aktiviti yang kuat terhadap S. aureus resisten
metisillin dan sederhana terhadap bakteria yang lain. Dalam penelitian
antioksidan menggunakan DPPH,
ketiga-tiga ekstrak daun M. lunu-ankenda mempamerkan
aktiviti yang kuat dengan nilai IC50 sebagai 7.8, 15.6 dan 7.8 μg/mL. Selain itu, ekstrak
petroleum eter daun M. clemensiae dan ekstrak
kloroform M. lunu-ankenda memberikan aktiviti sitotoksik yang kuat bila
diuji dengan sel leukemia promielositik manusia (HL60) dengan nilai IC50 masing-masing sebagai 2.8 dan 0.8 μg/mL
Kata kunci:
Antimikrob antioksidan; Melicope confusa; M.
clemensiae; M. lunu-ankenda; M. latifolia; sitotoksik.
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*Pengarang untuk surat-menyurat;
email: mawardi@fsas.upm.edu.my
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