Sains Malaysiana 38(5)(2009): 767–771

 

 

Biological Activities of Four Melicope Species

(Aktiviti Biologi bagi Empat Spesies Melicope)

 

Najihah Mohd Hashim1, Mawardi Rahmani1*, Hazar Bebe Mohd Ismail2,

Mohd Aspollah Sukari1, A. Manaf Ali3 & Gwendoline Ee Cheng Lian1

 

1Department of Chemistry, Universiti Putra Malaysia

43400 UPM Serdang, Selangor D.E., Malaysia

 

2Centre for Foundation Study in Science

Universiti Malaya, 50603 Kuala Lumpur, Malaysia

 

3Department of Biotechnology, Universiti Putra Malaysia

43400 UPM Serdang, Selangor D.E., Malaysia

 

Diserahkan: 13 Jun 2008 /  Diterima: 30 Disember 2008

 

 

ABSTRACT

 

The leaves and stem barks extracts of four plant species, Melicope confusa, M. clemensiae, M. lunu-ankenda and M. latifolia were screened for their antimicrobial, antioxidant and cytotoxic activities. Almost all extracts of M. clemensia exhibited moderate growth inhibition when tested against the three pathogenic fungi. However, when tested against pathogenic bacteria, the three leaves extracts showed strong activity against methicillin resistant S. aureus and moderate for the other bacteria. In the antioxidant assay by using DPPH, the three leaves extracts of M. lunu-ankenda exhibited significant activity with with IC50 values of 7.8, 15.6 and 7.8 μg/mL. In addition, the petroleum ether leaves extract of M. clemensiae and the chloroform leaves extract of M. lunu-ankenda showed strong cytotoxic activity when tested against human promyelocytic leukemia (HL60) cell line with IC50 values of 2.8 and 0.8 μg/mL, respectively.

 

Keywords: Antioxidant antimicrobial; cytotoxic; M. clemensiae; M. lunu-ankenda; M. latifolia; Melicope-confusa

 

ABSTRAK

 

Ekstrak daun dan kulit batang daripada empat spesies tumbuhan, Melicope confusa, M. Clemensiae, M. lunu-ankenda dan M. latifolia telah dilakukan penabiran aktiviti antimikrob, antioksidan dan sitotoksik. Hampir semua ekstrak M. clemensiae menghalang pertumbuhan secara sederhana bila diuji dengan kulat patogenik. Tetapi apabila diuji dengan bakteria patogenik, ketiga-tiga ekstrak daun menunjukkan aktiviti yang kuat terhadap S. aureus resisten metisillin dan sederhana terhadap bakteria yang lain. Dalam penelitian antioksidan menggunakan DPPH, ketiga-tiga ekstrak daun M. lunu-ankenda mempamerkan aktiviti yang kuat dengan nilai IC50 sebagai 7.8, 15.6 dan 7.8 μg/mL. Selain itu, ekstrak petroleum eter daun M. clemensiae dan ekstrak kloroform M. lunu-ankenda memberikan aktiviti sitotoksik yang kuat bila diuji dengan sel leukemia promielositik manusia (HL60) dengan nilai IC50 masing-masing sebagai 2.8 dan 0.8 μg/mL

 

Kata kunci: Antimikrob antioksidan; Melicope confusa; M. clemensiae; M. lunu-ankenda; M. latifolia; sitotoksik.

 

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*Pengarang untuk surat-menyurat; email: mawardi@fsas.upm.edu.my

 

 

 

 

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