Sains Malaysiana 38(5)(2009): 793–797
Kesan Ekstrak Akuas Lunasia amara Blanco Terhadap Kualiti Sperma,
Kesuburan dan Kelakuan Seksual Tikus Jantan
(Effects
of Aqueous Extract Lunasia amara Blanco on Sperm Quality,
Fertility
and Sexual Behaviour of Male Rats)
Muhamad Jafar Luthfi & Mahanem Mat Noor*
School of Biosciences and
Biotechnology
Faculty of Science and Technology, Universiti
Kebangsaan Malaysia
43600 UKM Bangi, Selangor
D.E., Malaysia
Diserahkan: 18 Disember 2008 / Diterima: 1 April 2009
ABSTRAK
Sanrego (Lunasia amara Blanco) digunakan masyarakat tempatan di Indonesia sebagai bahan afrodisiak dan mampu mengatasi masalah kesuburan lelaki. Penyelidikan ini mengkaji kesan pengambilan ekstrak akuas sanrego pada dos 30, 60 dan 90 mg/kg berat badan masing-masing terhadap bilangan, motiliti dan morfologi sperma serta tahap kesuburan dan afrodisiak tikus jantan. Kumpulan kawalan diberikan air suling. Semua perlakuan diberikan secara suap-paksa sekali sehari pada pukul 10.00 pagi-11.00 pagi selama 42 hari diikuti dengan analisis sperma dan ujian tahap kesuburan. Analisis sperma menunjukkan bahawa sanrego mempunyai kesan peningkatan bilangan sperma dengan gred motiliti terbaik (gred a) dengan signifikan tanpa mempengaruhi morfologi sperma. Pengujian tahap kesuburan menunjukkan peningkatan pada kumpulan haiwan perlakuan. Jumlah bilangan pemanjatan tikus jantan dengan perlakuan sanrego juga lebih tinggi berbanding kumpulan kawalan. Kajian ini mendapati tumbuhan sanrego berpotensi meninggikan kesuburan dan mempunyai kesan afrodisiak pada haiwan jantan.
Kata kunci: Afrodisiak; analisis sperma; kesuburan; Lunasia amara
ABSTRACT
Sanrego (Lunasia amara Blanco) has been used in the
folk medicine in Indonesia as aphrodisiac agent and to treat male fertility
problem. This research studied the effects of oral administration of aqueous
extract of sanrego at the doses of 30, 60 and 90
mg/kg body weight respectively on the sperm count, motility, morphology,
fertility and its aphrodisiac property in male rats. Control group was
administered with distilled water. Administration was given by oral gavaging between 10.00 am to 11.00 am daily for a period of
42 days followed by sperm analysis and fertility test. The sperm analysis
showed that the sanrego significantly increase the
sperm count with the best grade in motility (grade a) without affecting the
sperm morphology. The fertility rate was also increased in treated animals. The
number of mounting in male rats treated with sanrego was higher than that in control. This study shows the potential of sanrego to increase male fertility and has aphrodisiac
property.
Keywords:
Aphrodisiac; fertility, Lunasia amara; sperm analysis
RUJUKAN
Biggers, J.D.,
Whitten, W.K. & Whittingham, D. 1971. The culture of mouse embryos in vitro. In Methods
in Mammalian Embryology. Ed. JC Daniel. Freeman, San Francisco,
86-116.
Darise, M. 1999. Obat kuat: Berkuda dengan sanrego. Gatra 23: 1-3.
Dooher, G.B. &
Bennett, D. 1977. Spermiogenesis and spermatozoa in
sterile mice carrying different Lethal T/t locus haplotypes:
A transmission and scanning electron microscopic study. Biology of
Reproduction 17: 269-288.
Ebisch, I.M.W., Pierik, F.H.P., De Jong, F.H.,
Thomas, C.M.G. & Steegers-Theunissen, R.P.M.
2006. Does folic acid and zinc sulphate intervention
affect endocrine parameters and sperm characteristics in man? Int. J. Andr. 29(2): 339-345.
Elbetieha, A. & Da’as, S.I. 2003. Assessment of antifertility activities of abamectin pesticide in male rats. Ecotoxicology and Environmental Safety 55: 307-313.
Finlayson, A.C. & Prager, R.H.
1978. Biosynthetic studies on alkaloids in Lunasia amara. Aust. J. Chem. 31: 2751-2755.
Foster, S. & Duke, J.A. 2000. A Field
Guide to Medicinal Plants and Herbs. Second Edition. Houghton
Mifflin Company. Boston, 16-17.
Goodwin, S., Smith, A.F., Velasquez, A.A. & Horning,
E.C. 1959. Alkaloids of Lunasia umara Blanco. Isolation Studies. J. Am. Chem. Soc. 81: 6209-6213.
Gonzales, G.F., Gasco, M.,
Cordova, A., Chung, A., Rubio, J. & Villegas, L. 2004. Effect of Lepidium meyenii (Maca) on
spermatogenesis in male rats acutely exposed to high altitude (4340 m). Journal
of Endocrinology 180: 87-95.
Gonzales, C., Rubio, J., Gasco,
M., Nieto, J., yucra, S. & Gonzales, G.F. 2006. Effect of short-term and long-term treatment
with three ecotypes of Lepidium meyenii (Maca) on
spermatogenesis in rats. Journal of Ethnopharmacology 130(12): 448-454 C.
Hamadeh, M.E., Zeginiadov, T. & Rosenbaum, P. 2001. Predictive
value of sperm chromatin condensation (aniline blue staining) in the assessment
of male fertility. Archives of Andrology 46:
99-104.
Hamilton, D.W. 1975. Structure, function of
the epithelium lining the ductuli efferents, ductus epididymis and ductus deferens in the rat. In: Handbook of
Physiology, Section VII, Endocrinology, vol.5, Male
Reproductive System (eds D.W.
Hamilton and R.O. Greep). American Physiological Society. Washington, 259-301.
Kohn, F.M. 2001. Nonmedical and naturopathic
approaches to treatment of male fertility. Proceedings
of the 7th Andrology Symposium. Treatment of male infertility - viewpoints, controversies,
perspectives. Giessen,
Germany, 337.
Liu, D.y., Clarke, G.N. & Baker,
H.W.G. 1991. Relationship between sperm motility assessed with the
Hamilton-Thorn motility analyzer and fertilization rates in vitro. Journal
of Andrology 12: 231-239.
Luthfi, M.J., Mat Noor, M. & Latip. J. 2008. Effect of the aqueous extract of Lunasia amara Blanco on sperm quality and in vivo fertility of male
rats. Proceeding International Seminar on Chemistry. Universitas Sumatera Utara. Medan. Indonesia.
MacKay, D. 2004. Nutrients and botanicals for erectile
dysfunction: examining the evidence. Alternative Medicine Review 9(1):
4-16.
Mat Noor, M. & Luthfi, M.J. 2006. Penabiran lima herba yang berpotensi dalam meningkatkan parameter kesuburan haiwan jantan. Prosiding Seminar Bersama UKM-UNRI. Pusat Kembangan Pendidikan. Universiti Kebangsaan
Malaysia. Bangi.
Meyerson, B.J.,
Lindstrom, L., Nordstrom, E.B. & Agmo, A. 1973. Sexual motivation in the female rat after testosterone treatment. Physiology Behaviour 11: 421-428.
Nickel, L.N. 2001. Nature’s
Aphrodisiacs. USA: Crossing Press. 6-10.
Nordic Association for Andrology & European Society of Human Reproduction and
Embryology-Special Interest Group on Andrology 2002. Manual on basic semen analysis. Oxford: Oxford University Press. 1-27.
Nurlaila. 2000. Analisis Farmakognosi dan Fitokimia Simplia Daun Maitan (Lunasia amara Blanco). Tesis undergraduate. Fakultas Farmasi, Universitas Padjajaran. Bandung.
Pei, J., Strehler, E., Noss, U., Abt, M., Piomboni, P., Baccetti, B. & Sterzik, K. 2005. Quantitative evaluation of spermatozoa ultrastructure after acupuncture treatment for idiopathic male infertility. Fertility
and Sterility 84(1): 141-147.
Prasad, M.R.N., Chinoy, N.J.
& Kadam, K.M. 1972. Changes in succinic dehydrogenase levels in the rat epididymis under normal and altered physiologic conditions. Fertility and Sterility 23(3):
186-190.
Sinclair, S. 2000. Male infertility : Nutritional and Environmental Considerations. Alternative Medicine Review 5(1):
28-38.
Sutanto, H. 2002. Uji efek androgenik ekstrak kayu sanrego (Lunasia amara Blanco) pada tikus jantan. Tesis Sarjana. Unpublished. Universitas Katholik Widya Mandala Surabaya.
World Health Organization 1999. Laboratory manual for the examination of
human semen and semen-cervical mucus interaction. New york: Cambridge University Press.
*Pengarang untuk surat menyurat;
email: mahanem@ukm.my
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