Sains Malaysiana 38(6)(2009): 929–934

Profil Darah dan Urin Tikus Teraruh Hipertensi yang diberi

Suplemen Puri Jambu Batu Merah (Psidium guajava)

(Blood and Urine Profiles of Spontaneous Hypertensive Rats Supplemented with Pink Guava

(Psidium guajava) Puree)

Norazmir Md Nor

Department of Nutrition and Dietetics, Faculty of Health Sciences

Universiti Teknologi MARA, Jalan Othman, 46000 Petaling Jaya, Selangor, Malaysia

Ayub Mohd Yatim* & Mamot Said

School of Chemical Sciences & Food Technology, Faculty of Science & Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

Diserahkan: 12 Januari 2009 / Diterima: 15 April 2009

ABSTRAK

Kajian ini dijalankan untuk menentukan kesan pemberian suplemen puri jambu batu merah (Psidium guajava) ke atas profil darah dan urin tikus teraruh hipertensi. Sebanyak 24 ekor tikus dibahagi 4 kumpulan (kawalan, CG (air suling); dos rendah, LDG (0.5 g/kg berat badan); dos sederhana, MDG (1.0 g/kg berat badan); dos tinggi, HDG (2.0 g/kg berat badan). Tikus diberi puri jambu batu merah secara suap-paksa selama 28 hari dan diletakkan dalam sangkar individu. Berat organ dan profil lipid serum tidak berbeza secara signifikan antara kumpulan rawatan. Nilai glukosa darah (mmol/l) MDG (5.53±0.69) lebih tinggi secara signifikan berbanding CG (4.53±0.39) dan LDG (4.80±0.54). Tiada perbezaan signifikan pada status antioksida total, urea, fosfat alkali dan globulin antara kumpulan rawatan. Terdapat perbezaan signifikan pada hematologi darah, bilirubin total, gama-glutamil transpeptidase (GGT), protein total, albumin dan nisbah albumin:globulin antara CG dan kumpulan rawatan. Sel darah merah dan platlet bagi LDG (10.95±0.57 x 10¹²/l; 941.17±139.0 x 10⁹/l) lebih tinggi berbanding CG (10.05±0.63 x 10¹²/l; 858.83±163.4 x 10⁹/l) masing-masing. LDG (3.51 umol/l) mempunyai kepekatan bilirubin total lebih rendah berbanding CG (4.25 umol/l). Tahap GGT pula lebih tinggi pada HDG (8.67 U/l) berbanding CG dan MDG (7.00 U/l). Nisbah albumin:globulin lebih rendah pada CG (1.50) berbanding MDG (1.36) dan HDG (1.37). Julat pH urin dan kandungan urobilinogen dalam darah antara 7.3-8.0 dan 2.8-3.2 μmol/l masing-masing. Tiada kehadiran glukosa dan bilirubin pada semua urin kumpulan rawatan. Suplemen puri jambu batu merah (Psidium guajava) ke atas tikus SHR menunjukkan tiada kesan signifikan terhadap profil lipid serum, urin, berat organ dan tahap glukosa darah.

Kata kunci: Jambu batu merah; profil darah; profil urin; tikus teraruh hipertensi

ABSTRACT

The objectives of this study were to investigate the effects of pink guava (Psidium guajava) puree on blood and urine profiles of Spontaneous Hypertensive Rats (SHR). Twenty-four male SHR rats were divided into four groups (control, CG (distilled water); low dosage group, LDG (0.5 g/kg body weight); medium dosage group, MDG (1.0 g/kg body weight); and high dosage group, HDG (2.0g/kg body weight)). The rats were given pink guava puree via force-feeding and fed ad libitum for 28 days in individual cages. Organ weights and serum lipid profiles were not significantly different between the groups. Blood glucose value (mmol/l) for MDG (5.53±0.69) was significantly higher than CG (4.53±0.39) and LDG (4.80±0.54). Blood chemistry analysis showed no significant difference in total antioxidant status, urea, alkaline phosphate and globulin between the groups. There were significant differences in the blood hematology, concentrations total bilirubin, gamma-glutamyl transpeptidase (GGT), total protein, albumin and albumin:globulin ratio between CG and treated groups. LDG's red blood cell (10.95±0.57 x 10¹²/l) and platelet (941.17±139.0 x 10⁹/l) were higher compared to CG (10.05±0.63 x 10¹²/l; 858.83±163.4  x 10⁹/l) respectively. LDG (3.51 umol/l) had lower total bilirubin concentration than CG (4.25 umol/l). GGT level was significantly higher in HDG (8.67 U/l) compared to CG and MDG (7.00 U/l). Albumin:globulin ratio values were significantly lower in CG (1.50) than MDG (1.36) and HDG (1.37). In conclusion, this study shows pink guava (Psidium guajava) puree supplementation did not have significant effects on serum lipid and urine profiles, organ weight and blood glucose concentration of SHR.

Keywords: Pink guava; blood profile; spontaneous hypertensive rats; urine profile

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*Pengarang untuk surat-menyurat; email: amy@ukm.my