Sains
Malaysiana 38(6)(2009): 939–945
Identification of
Immune-related Genes by Analysis of Spleen Expressed Sequence Tags from the
Asian Seabass, Lates calcarifer
(Pengenalpastian
Gen Berkaitan Imun melalui Analisis Penanda Jujukan Terungkap Limpa
daripada
Ikan Siakap, Lates calcarifer)
Nurul-Yuziana
Mohd-Yusof, Chee-Choong Hoh, Adura Mohd-Adnan & Kiew-Lian Wan*
School
of Biosciences and Biotechnology
Faculty
of Science and Technology, Universiti Kebangsaan Malaysia
43600 UKM Bangi, Selangor, Malaysia
Malaysian
Genome Institute
Heliks
Emas Block, UKM-MTDL Technology Centre
Universiti
Kebangsaan Malaysia, 43600 Bangi Selangor D.E.,
Malaysia
Diserahkan:
7 Januari 2009 / Diterima: 8 April 2009
ABSTRACT
The Asian
seabass (Lates calcarifer) is one of the
most economically important aquaculture fish species in South East Asia. While
the biology of the Asian seabass is widely studied, relatively little
information is available at the molecular level. This lack of molecular
information represents one obstacle to rapid progress in the study of immune
responses particularly under aquaculture conditions. In light of this
situation, we have undertaken an expressed sequence tag (EST) project on the Asian seabass spleen, the secondary lymphoid
organ, for the identification of immune-related genes. A total of 2932 ESTs were generated and grouped into 1063 unique transcripts (UTs), which consisted of 104 consensi and 959 singletons. Of
these, 51.3% (545/1063) matched to previously identified genes, while 48.7%
(518/1063) showed no match. Of the 545 homologous UTs, 102 (9.6%) can be putatively identified as immune-related
genes. The identification of the putative immune-related genes provides a
meaningful framework in the effort to comprehend the Asian seabass immune
system that may lead to an increase in the understanding of the defense
mechanisms of and our abilities to manage this fish species.
Keywords:
Fish immune system; innate immunity; transcriptome
ABSTRAK
Ikan siakap (Lates calcarifer) merupakan salah satu daripada
spesies ikan akuakultur yang terpenting daripada segi ekonomi di rantau Asia
Tenggara. Walaupun biologi ikan siakap banyak dikaji, namun maklumat pada
peringkat molekul mengenai biologi spesies ikan ini masih kurang. Kekurangan
maklumat molekul ini merupakan salah satu sebab yang menghalang kemajuan dalam
kajian gerak balas imun terutamanya dalam sekitaran akuakultur. Memandangkan
keadaan ini, kami telah menjalankan projek penanda jujukan terungkap (EST) terhadap limpa ikan siakap, yang merupakan organ limfoid
sekunder, bagi mengenalpasti gen berkaitan imun. Sejumlah 2932 EST telah dijana dan dikelompokkan ke dalam 1063 transkrip unik (UT), yang terdiri daripada 104 konsensus dan 959 tunggalan.
Daripada jumlah ini, 51.3% (545/1063) mempunyai padanan terhadap gen yang
pernah dicirikan, manakala 48.7% (518/1063) tidak menunjukkan sebarang padanan.
Di antara 545 UT yang
mempunyai homologi, 102 (9.6%) boleh dikenalpasti secara putatif sebagai gen
berkaitan imun. Pengenalpastian gen berkaitan imun putatif menyediakan kerangka
yang berguna dalam usaha untuk memahami sistem imun ikan siakap yang mungkin
dapat membawa kepada peningkatan pemahaman mengenai mekanisme pertahanan dan
keupayaan kita untuk mengurus spesies ikan ini.
Kata kunci:
Keimunan inat; sistem keimunan ikan; transkriptom
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*Pengarang
untuk surat-menyurat; email: klwan@ukm.my
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