Sains
Malaysiana 40(10)(2011): 1087–1095
Genetic Characterization of Two
Mahseer Species (Tor douronensis and
Tor tambroides) Using
Microsatellite Markers from Other Cyprinids
(Pencirian Genetik
dua Spesies Mahseer (Tor douronensis dan Tor tambroides)
Menggunakan Penanda
Mikrosatelit daripada Siprinid yang Berbeza)
Yuzine
B. Esa
Department
of Zoology, Faculty of Resource Science and Technology, Universiti Malaysia
Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Khairul
Adha A. Rahim
Department
of Aquatic Sciences, Faculty of Resource Science and Technology, Universiti
Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Siti
Shapor Siraj*, Muhammad Fadhil Syukri
Department
of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400
Serdang Selangor D.E., Malaysia
Siti
Khalijah Daud, Ho Ghim Chong
Biology Department , Faculty of Science, Universiti
Putra Malaysia, 43400 Serdang, Selangor D.E., Malaysia
Tan
Soon Guan
Department
of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular
Sciences, Universiti Putra Malaysia, 43400 Serdang Selangor D.E., Malaysia
Diserahkan: 23
Ogos 2010 / Diterima: 14 Disember 2010
ABSTRACT
This study examined the
genetic characteristics of twenty-six microsatellite primers developed from
three cyprinid fishes (Cyprinus carpio Linnaeus, Barbus
barbus Linnaeus and Barbonymus gonionotus Bleeker) in two indigenous
mahseer. The Tor douronensis Valenciennes were randomly collected from two locations in Sarawak (N=52),
while Tor tambroides Bleeker were obtained from Peninsular
Malaysia (N=56). A total of ten and twelve primers were successfully amplified
producing four and five polymorphic loci in T. douronensis and T.
tambroides, respectively. The number of alleles per locus ranging from 2 to
5 in T. douronensis and 2 to 7 in T. tambroides. A significant
deviation from Hardy-Weinberg equilibrium (HWE)
was observed at three loci (Barb37, Barb59 and Barb62) in one or more
populations in T. tambroides while two loci (Barb37 and Barb62) were
deviated in T. douronensis population of Batang Ai. Population structure
analysis showed low level of inter-population genetic differentiation in both
mahseer. Overall, the identified microsatellite loci should be useful in
analysing T. douronensis and T. tambroides natural populations.
Keywords: Cross-species
study; genetic characterization; mahseer; microsatellites
ABSTRAK
Kajian ini meneliti
pencirian genetik dua puluh enam primer mikrosatelit yang dibentuk daripada
tiga ikan siprinid (Cyprinus carpio Linnaeus, Barbus barbus Linnaeus and Barbonymus
gonionotus Bleeker) ke atas dua ikan kelah indigenus. Ikan kelah Tor
douronensis Valenciennes telah dipilih secara rawak dari dua tempat yang
berbeza di Sarawak (N=52), manakala Tor tambroides Bleeker pula telah
dikumpul secara rawak dari Semenanjung Malaysia (N=56). Sejumlah sepuluh primer
berjaya diamplifikasi menghasilkan empat lokus polimorfik pada Tor
douronensis; dan dua belas primer pada Tor tambroides dengan lima
lokasi polimorfik. Nombor alel per lokus berjulat di antara dua hingga lima
dalam Tor douronensis dan dua hingga tujuh dalam Tor tambroides.
Penyimpangan daripada keseimbangan Hardy-Weinberg (HWE)
yang signifikan telah dijumpai pada tiga lokus (Barb37, Barb59 dan Barb62) di
dalam satu atau lebih populasi T. tambroides manakala dua lokus (Barb37
dan Barb62) mengalami penyimpangan dalam populasi T. douronensis dari
Batang Ai. Analisis struktur populasi menunjukkan tahap perbezaan genetik yang
rendah di peringkat inter-populasi dalam kedua-dua ikan kelah. Keseluruhannya,
lokus mikrosatelit yang dikenalpasti berguna untuk menganalisis secara mendalam
populasi semulajadi ikan T. douronensis dan T. tambroides.
Kata kunci: Kajian spesies-silang; kelah; mikrosatelit; pencirian
genetic
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*Pengarang untuk surat-menyurat; email:
shaporsiraj@yahoo.com
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