Sains
Malaysiana 41(12)(2012): 1549–1556
Kesan Anti-hiperglisemia Ekstrak Metanol Gynura procumbens terhadap
Kesuburan dan Libido Tikus Jantan Teraruh Diabetes
(Anti-hyperglycemic Effect of Gynura
procumbens Methanolic Extract on Fertility
and Libido of Induced Diabetic Male Rats)
Mahanem Matnoor*
& N. Anirahayu Mohd Radzuan
Pusat Pengajian Biosains dan Bioteknologi, Fakulti Sains dan
Teknologi
Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul
Ehsan, Malaysia
Diserahkan: 13 Jun 2012 / Diterima: 6 Ogos 2012
ABSTRAK
Gynura procumbens (GP) adalah sejenis
tumbuhan herba yang digunakan bagi merawat pelbagai jenis penyakit seperti
rheumatoid, darah tinggi, masalah ginjal dan juga diabetes. Diabetes Mellitus (DM)
adalah sejenis penyakit kronik dan salah satu komplikasi penyakit ini ialah
masalah kesuburan dan libido. Kajian ini dijalankan
untuk mengenal pasti potensi ekstrak GP dalam mengatasi masalah kesuburan dalam
kalangan pesakit diabetes. Ekstrak metanol GP dengan
fraksi etil asetat digunakan dalam kajian ini. Aruhan
diabetes dilakukan dengan suntikan dadah Streptozotosin sebanyak 55 mg/kg berat
tikus secara intravena. Empat kumpulan tikus teraruh diabetes dirawat
dengan 50 mg/kg GP (n=12), 300 mg/kg GP (n=12) dan 300 mg/kg metformin (n=12).
Kumpulan ke-empat adalah kumpulan diabetik tanpa sebarang rawatan dan dijadikan
kawalan negatif (n=12) dan satu kumpulan tikus normal yang diberi air
suling dijadikan kawalan normal (n=12). Satu minggu
selepas rawatan, enam ekor tikus daripada setiap kumpulan diuji kelakuan
seksual sebelum dikorbankan untuk kajian kesuburan. Ujian yang sama dilakukan ke atas baki haiwan dalam setiap kumpulan
pada minggu kedua rawatan. Pada semua dos rawatan, ekstrak GP menunjukkan
kesan anti-hiperglisemia sebagaimana kumpulan metformin. Rawatan dengan
dos rendah GP (50 mg/kg) selama dua minggu mencatatkan bilangan sperma yang
paling tinggi iaitu sebanyak (35.52 ± 6.54) × 106 sperma berbanding
kawalan diabetik, (23.72 ± 5.12) × 106 sperma. Kumpulan ini
turut mencatatkan peratus jumlah pergerakan sperma gred a (paling progresif)
yang tinggi iaitu sebanyak 38.41% berbanding 12.79% bagi kumpulan kawalan
diabetik. Tikus yang dirawat dengan GP turut menunjukkan
peningkatan kesan libido dengan tempoh pendam pemanjatan dikurangkan dan
bilangan pemanjatan meningkat berbanding tikus kawalan diabetik. Kajian ini menunjukkan ekstrak metanol GP dengan fraksi etil
asetat ini mampu meningkatkan kesuburan dan libido tikus diabetik pada dos yang
rendah (50 mg/kg) serta dalam tempoh yang singkat.
Kata kunci: Diabetes; Gynura procumbens (GP); kelakuan seksual;
kualiti sperma
ABSTRACT
Gynura procumbens (GP) is a type of
herbal plant used to treat numerous types of diseases such as rheumatoid,
hypertension, kidney failure as well as diabetes. Diabetes mellitus (DM)
is one of the most common chronic diseases and one of its complication is infertility and libido problems among male patients. This study aimed to
evaluate the potential of GP extract in enhancing fertility and libido
among diabetic patients. Ethyl acetate fraction of GP methanol extract was
used. Diabetic inductions were carried out using 55 mg/kg Streptozotocin via
intravein injection. Four groups of diabetic induced rats were treated with 50
mg/kg GP extract (n=12), 300 mg/kg GP extract (n=12)
and 300 mg/kg metformin (n=12) respectively. The fourth diabetic group
acted as negative control (without treatment) and one normal group was given
distilled water acted as control normal group (n=12). Each of these
groups underwent sexual behavioral tests prior to being sacrificed for
fertility test. Six rats from each of these groups were sacrificed after one
week while the rest were sacrificed after two weeks of treatment. All doses of GP exhibited
anti-hyperglycemia similar as to metformin. Treatment with low dose GP (50
mg/kg) for two weeks produced the highest number of sperm count (35.52 ±
6.54)×106 in
comparison with diabetic control group (23.72 ± 5.12)×106. Most of the sperm
motility for this group was in grade a (most progressive) with 38.41% while for
diabetic control group, only 12.79%. The mounting latency for diabetic rats
treated with GP decreases while the mounting frequency increases significantly in
comparison with the diabetic control group suggesting that GP extract possesses
aphrodisiac effect. These results showed that ethyl acetate fraction of GP methanol
extract enhances the fertility and libido of diabetic rats efficiently even at
low dose (50 mg/kg) in a short period of time.
Keywords: Diabetes; Gynura procumbens (GP); sexual behavior; sperm quality
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*Pengarang untuk surat-menyurat; email: mahanem@ukm.my