Sains Malaysiana 41(6)(2012): 721–729

Changes in Hepatic Phosphoprotein Levels in Mice Infectedwith Plasmodium berghei

(Perubahan Aras Fosfoprotein Hepar dalam Mencit Terinfeksi Plasmodium berghei)

Pramila Maniam, Zainalabidin Abu Hassan, Noor Embi & Hasidah Mohd Sidek*

School of Biosciences & Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

Diserahkan: 7 Februari 2011 / Diterima: 5 Januari 2012

ABSTRACT

Hepatic phosphoprotein levels are altered in mouse liver as a manifestation of bacteria, virus or parasite infection. Identification of signaling pathways mediated by these hepatic proteins contribute to the current understanding of the mechanism of pathogenesis in malarial infection. The present study was undertaken to evaluate the changes in hepatic phosphoprotein levels during Plasmodium berghei infection. Our study revealed changes in levels of three hepatic phosphoproteins following P. berghei infection compared to non-infected controls. Peptide fragment sequence analysis using tandem mass spectrometry (MS/MS) showed these hepatic proteins to be homologs to haemoglobin beta (HBB), class Pi glutathione S-tranferase (GSTPi) and carbonic anhydrase III (CAIII) proteins of Mus musculus species respectively from the NCBInr sequence database. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis predicted the involvement of these proteins in specific pathways in Mus musculus species; GSTPi in glutathione and drug metabolism and CAIII in nitrogen metabolism. This shows that P. berghei infection affects similar signaling pathways as those reported in other pathogenic infections such as that related to GSTPi and CAIII in response to oxidative stress.

Keywords: Chloroquine; hepatomegaly; malaria; phosphoprotein; Plasmodium berghei

ABSTRAK

Aras fosfoprotein hepar mencit berubah semasa manifestasi infeksi oleh bakteria, virus dan parasit. Pengenalpastian tapak jalan pengisyaratan yang diperantara oleh protein hepar boleh menyumbang kepada pemahaman terhadap patogenesis infeksi malaria. Kajian ini dilakukan untuk menentukan perubahan aras fosfoprotein hepar semasa infeksi Plasmodium berghei. Dalam kajian ini, perubahan aras tiga fosfoprotein hepar telah diperhatikan sewaktu infeksi P. berghei berbanding kawalan tanpa infeksi. Analisis jujukan fragmen peptida menggunakan spektrometri jisim tandem (MS/MS) menunjukkan protein hepar tersebut terdiri daripada homolog kepada hemoglobin beta (HBB), glutation S-tranferase kelas Pi (GSTPi) dan karbonik anhidrase III (CAIII) daripada spesies Mus musculus masing-masing dari pangkalan data jujukan NCBInr. Analisis tapak jalan menggunakan pangkalan data Kyoto Encyclopedia of Genes and Genomes (KEGG) meramalkan penglibatan GSTPi dalam metabolisme glutation dan dadah; dan protein CAIII dalam metabolisme nitrogen, kesemuanya dalam spesies Mus musculus. Kajian ini menunjukkan bahawa infeksi P. berghei memberi kesan terhadap tapak jalan pengisyaratan yang telah dilaporkan terlibat dalam infeksi patogen lain umpamanya tapak jalan berkaitan GSTPi dan CAIII sebagai respons terhadap tekanan oksidatif.

Kata kunci: Fosfoprotein; hepatomegali; klorokuin; malaria; Plasmodium berghei

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*Pengarang surat-menyurat; email: hasidah@ukm.my