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Synergistic and Antagonistic Effects of Zinc Bioaccumulation with Lead and Antioxidant Activities in Centella asiatica (Kesan Sinergistik dan Antagonistik oleh Bioakumulasi Zink dengan
Plumbum dan Aktiviti Antioksidan di Centella asiatica)
G.H. ONG1, C.K.
YAP1*, M.
MAZIAH2 & S.G. TAN3
1Department of Biology, Faculty of Science, Universiti
Putra Malaysia 43400 UPM, Serdang, Selangor, Malaysia 2Department of Biochemistry, Faculty of Biotechnology
and Biomolecular Sciences Universiti Putra Malaysia, 43400 UPM, Serdang,
Selangor, Malaysia 3Department of Cell and Molecular Biology, Faculty of Biotechnology
and Biomolecular Sciences Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
Diserahkan: 13 Jun 2012 /Diterima: 9 April 2013
ABSTRACT
This study was carried out by using Centella asiatica grown using a hydroponic
system under laboratory conditions to study synergistic and antagonistic
effects of Zn bioaccumulation with added Pb and the changes in antioxidant
activities in leaves and roots of C. asiatica. The antioxidant
activities included superoxide dismutase (SOD),
catalase (CAT), ascorbate peroxidase (APX)
and guaiacol peroxidase (GPX). The treatments Zn (2 ppm) + Pb
(0.4 ppm) and Zn (4 ppm) + Pb (0.6 ppm) increased the accumulation of Zn in
leaves by 14.06 and 16.84%,
respectively, but decreased by 7.36% uptake in roots (Zn 4 ppm + Pb 0.6 ppm).
This showed that Pb and Zn acted synergistically to Zn accumulation in leaves
but antagonistically in roots. CAT and SOD activities
in leaves were increased when Zn was added together with Pb. In roots, CAT, APX and SOD activities were increased but GPX was
decreased. Owing to their sensitivities to Zn with Pb, SOD and CAT could be used as biomarkers to monitor the toxicity of Pb and Zn
exposure in the leaves and roots of C. asiatica.
Keywords: Antagonistic; antioxidant activities; Centella asiatica; synergistic
ABSTRAK
Kajian ini telah dijalankan dengan Centella asiatica secara hidroponik dalam keadaan
makmal untuk mengkaji kesan sinergistik dan antagonistik bioakumulasi
Zn ditambah dengan Pb dan perubahan dalam aktiviti antioksidan dalam
daun dan akar C. asiatica. Aktiviti antioksidan termasuk
superokside dimustase (SOD),
katalase (CAT), peroksidase askorbat (APX)
dan peroksidase guaiacol (GPX). Rawatan Zn (2 ppm) + Pb (0.4 ppm)
dan Zn (4 ppm) + Pb (0.6 ppm) menunjukkan pengumpulan Zn dalam daun
sebanyak 14.06 dan 16.84%
masing-masing tetapi menurun sebanyak 7.36% dalam pengambilan akar
(Zn 4 ppm + Pb 0.6 ppm). Ini menunjukkan bahawa Pb dan Zn bertindak
secara sinergistik untuk pengumpulan Zn dalam daun tetapi antogonistik
dalam akar. Aktiviti CAT
dan SOD
dalam daun meningkat apabila Zn ditambah bersama dengan
Pb. Dalam akar, CAT, APX dan SOD telah
meningkat tetapi GPX telah menurun. Disebabkan sentiviti
kepada Zn dengan Pb, SOD dan CAT boleh
digunakan sebagai penanda biologi untuk memantau ketoksikan pendedah
Pb dan Zn dalam daun dan akar C. asiatica.
Kata kunci: Aktiviti antioksidan; antagonistik; Centella asiatica; sinergistik
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