Sains
Malaysiana 42(2)(2013): 123–127
Total
Phenolics and Antioxidant Activities of Pouteria campechiana Fruit Parts
(Fenolik Jumlah dan Aktiviti Antioksida Sebatian Bahagian
Buah Pouteria campechiana)
K.W.
Kong1, H.E. Khoo2, N.K. Prasad3, L.Y. Chew4,
I. Amin2,5*
1Department
of Molecular Medicine, Faculty of Medicine, University of Malaya
50603
Kuala Lumpur, Malaysia
2Department
of Nutrition and Dietetics, Faculty of Medicine and Health Sciences
Universiti
Putra Malaysia, UPM Serdang, 43400 Selangor, Malaysia
3Monash
University, Chemical Engineering Discipline, School of Engineering
Jalan
Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia
4School
of Biosciences, Taylor’s University, Taylor’s Lakeside Campus
No.
1, Jalan Taylor’s, 47500 Subang Jaya, Selangor, Malaysia
5Laboratory
of Halal Science Research, Halal Products Research Institute
Universiti
Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Diserahkan:
26 April 2012 / Diterima: 10 Oktober 2012
ABSTRACT
This study aimed to evaluate the total phenolics and antioxidant
capacities of the seeds, pulp and peel of Pouteria campechiana fruit using
three extraction solvents (water, 70% methanol and 70% ethanol). Among them,
70% ethanol exhibited the best solvent for yielding highest total phenolic
content (TPC), total flavonoid content (TFC)
and antioxidant activities. The result showed that 70% ethanol extract from the
peel contained the highest TPC (2304.7 mg gallic acid
equivalent/100 g dw) while the pulp has the highest TFC (6414.03
mg rutin equivalent/100 g dw). The antioxidant activities of the pulp and peel
ethanolic extracts were high as determined using
2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
radical cation assay (49.60 and 49.56 mmoL TE/100
g dw) and ferric reducing antioxidant power assay (43.88 and 42.94 Fe2+/100
g dw) but not for seeds. However, their diphenyl-1-picrylhydrazyl (DPPH)
radical scavenging activities were ~88%. Thus, the pulp and peel of P.
campechiana fruit can be utilized as natural source for antioxidant
components.
Keywords: Antioxidant activity; Pouteria campechiana; solvent
extraction; total phenolics
ABSTRAK
Kajian ini bertujuan untuk menentukan fenolik jumlah dan keupayaan antioksidan daripada biji, isi dan kulit buahPouteria campechiana dengan menggunakan tiga pelarut pengekstrakan (air, 70% metanol dan 70% etanol). Antara pelarut tersebut,
70% etanol adalah yang terbaik untuk mendapatkan kandungan fenolik jumlah (TPC), kandungan flavonoid jumlah (TFC) dan mempunyai aktiviti antioksidan yang tertinggi. Keputusan kajian menunjukkan bahawa ekstrak 70% etanol daripada kulit buah mengandungi TPC tertinggi (2304.7 mg persamaan asid galik /100 g bk), manakala isinya mengandungi TFC yang tertinggi (6414.03 mg persamaan rutin /100 g bk). Aktiviti antioksidan ekstrak etanol isi dan kulit yang ditentukan oleh asai radikal kation ABTS (49.60 and 49.56 mmoL TE/100
g bk) dan asai kuasa antioksidan penurunan ferik (43.88 and
42.94 Fe2+/100 g bk) adalah tinggi tetapi tidak untuk bijinya. Aktiviti merantas radikal DPPH adalah ~88%. Maka, isi dan kulit buah P. campechiana boleh digunakan sebagai sumber semula jadi untuk mendapatkan sebatian antioksidan
Kata kunci: Aktiviti antioksidan; jumlah
fenolik; pelarut pengestrakan; Pouteria
campechiana
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*Pengarang
untuk surat-menyurat; email: amin@medic.upm.edu.my
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