Sains Malaysiana 42(4)(2013): 529–535
Molecularly
Imprinted Polymer Synthesis Using RAFT Polymerisation
(Sintesis Polimer Molekul Tercetak Menggunakan Pempolimeran RAFT)
Peter
A.G. Cormack* & Faizatul Shimal Mehamod
WestCHEM,
Department of Pure and Applied Chemistry, University of Strathclyde
Thomas
Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, Scotland
Faizatul
Shimal Mehamod
Department
of Chemical Sciences, Faculty of Science and Technology
Universiti
Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
Diserahkan:
26 Jun 2011 / Diterima: 9 Mei 2012
ABSTRACT
In this paper, the synthesis and characterisation of
caffeine-imprinted polymers are described. The polymers were prepared in
monolithic form via both reversible addition-fragmentation chain-transfer (RAFT)
polymerisation and conventional free radical polymerisation, using methacrylic
acid and ethylene glycol dimethacrylate as the functional monomer and
crosslinking agent, respectively. The potential benefits in applying RAFT polymerisation techniques towards the synthesis of molecularly
imprinted polymers (MIPs) are explored and elucidated. The
pore structures of the polymers produced were characterised by nitrogen
sorption porosimetry and the molecular recognition properties of representative
products were evaluated in high-performance liquid chromatography (HPLC)
mode. Molecular imprinting effects were confirmed by analysing the relative
retentions of analytes on imprinted and non-imprinted HPLC stationary
phases. It was found that a caffeine-imprinted polymer synthesised by RAFT polymerisation was superior to a polymer prepared using a
conventional synthetic approach; the imprinting factor and column efficiency
were found to be higher for the former material.
Keywords: Caffeine; molecular recognition; molecularly imprinted
polymers; novel stationary phases; RAFT polymerisation
ABSTRAK
Dalam penyelidikan ini, sintesis dan pencirian terhadap polimer
tercetak-kafein telah diterangkan. Polimer tersebut telah disediakan dalam
bentuk monolitik melalui pempolimeran tambahan-fragmentasi rantai pindah boleh
balik (RAFT) dan pempolimeran konvensional radikal bebas,
menggunakan asid metaakrilik sebagai monomer berfungsi dan etilena glikol
sebagai ejen taut silang. Potensi yang dapat dimanfaatkan dengan menggunakan
teknik pempolimeran RAFT dalam sintesis polimer molekul
tercetak (MIPs) telah diterokai dan difahami. Struktur liang
polimer yang terhasil telah dicirikan dengan menggunakan porosimeter penyerapan
nitrogen manakala pengecaman sifat molekul produk tersebut telah dinilai dalam
mod kromatografi cecair berprestasi tinggi (HPLC).
Hasil pencetakan molekul dikenal pasti dengan menganalisis perbezaan relatif
antara puncak analit-tercetak dan analit-tidak tercetak dalam fasa gerak HPLC.
Didapati polimer tercetak-kafein melalui pempolimeran RAFT adalah
lebih baik daripada polimer yang disintesis melalui kaedah konvensional; kesan
pencetakan dan kecekapan kolum didapati lebih tinggi bagi bahan yang pertama
tadi.
Kata kunci: Fasa gerak
terbaharu; kafein; pempolimeran RAFT;
pengecaman molekul; polimer molekul tercetak
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*Pengarang untuk
surat-menyurat; email: Peter.Cormack@strath.ac.uk