Sains Malaysiana 42(9)(2013): 1267–1272
In vitro Antifungal Activities and Phytochemical Analysis of Filamentous
White-rot Fungi, Schizophyllum commune
(Aktiviti Anti-kulat Pereput In vitro dan Analisis
Fitokimia bagi Kulat Pereput Putih Berfilamen, Schizophyllum
commune)
Yi Peng Teoh
& Mashitah Mat Don*
School of Chemical Engineering, Universiti Sains Malaysia, 14300
Nibong Tebal
Seberang Perai South, Penang, Malaysia
Diserahkan:
23 Julai 2012/ Diterima: 16 Disember 2012
ABSTRACT
In this study, the in-vitro antifungal activity and phytochemical analysis of Schizophyllum
commune extracts have been investigated. The antifungal activity was tested
against 11 species of selected wood degrading fungi of rubberwood. The results
showed that water, methanol and ethanol extracts significantly inhibited the
growth of wood decaying fungi with minimum inhibitory concentration (MIC)
ranges 0.16-5.00 μg/μL. P. sanguineus was
found as the strongest wood degrading fungus where it required the highest
concentration of S. commune crude extracts (≥ 5.00 μg/μL) to
inhibit its mycelia growth. Phytochemicals
analysis revealed that the extracts contained flavonoid, phenol and saponin.
The methanol extracts of S. commune was then applied on the rubberwood blocks
and found that the growth of P. sanguineus was inhibited effectively at
5.00 μg/μL.
Keywords: Antifungal activity; minimum inhibitory concentration (MIC);
rubberwood block; Schizophyllum commune;
wood decaying fungi
ABSTRAK
Dalam kajian ini, aktiviti anti-kulat in vitro dan analisis fitokimia bagi kulat Schizophlyllum
commune telah dikaji. Aktiviti anti-kulat telah diuji ke
atas 11 spesies kulat pereput putih kayu yang terpilih. Keputusan menunjukkan ekstrak air, metanol dan etanol boleh merencat
pertumbuhan kulat pereput putih kayu dengan kepekatan rencatan minimum (KMM)
di dalam julat 0.16-5.00 μg/μL.P. sanguineus telah dikenal pasti sebagai kulat pereput putih kayu yang terkuat kerana ia
memerlukan kepekatan ekstrak kulat S. commune yang tinggi (≥5.00
μg/μL) untuk merencat pertumbuhan misilianya. Analisis
fitokimia pula menunjukkan yang ekstrak ini mengandungi flavanoid, asid
karbolik dan saponin. Ekstrak methanol daripada kulat S. commune ini
juga telah diuji keberkesanannya ke atas blok kayu
getah dan didapati kulat P. sanguineus telah terencat secara efektif
pada 5.00 μg/μL.
Kata kunci: Aktiviti antikulat; blok kayu getah; kepekatan merencat minimum (KMM);
kulat pereput kayu; Schizophyllum commune
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*Pengarang
untuk surat-menyurat; email: chmashitah@eng.usm.my
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