Sains
Malaysiana 43(10)(2014): 1543–1547
Proximate Analysis and
Anti-Proliferative Properties of Vitex negundo L.
(Analisis Proksimat dan Sifat
Perencatan Proliferatif Vitex negundo L.)
RABETA MOHD. SALLEH1*, NEDA GANJALI DASHTI1 & ONG MING THONG2
1Food
Technology Division, School of Industrial Technology, Universiti Sains Malaysia
11800 Minden, Penang, Malaysia
2Institute
for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden,
Penang
Malaysia
Diserahkan: 19 Julai 2013/Diterima: 7
Februari 2014
ABSTRACT
The study of the proximate analysis on the
leaves of Vitex negundo L. (VN)
was done to gain the preliminary data of the anti-proliferative properties on
cancer cell lines. Aqueous and organic extracts of the leaves of VN were used
to identify its cytotoxic effect on six types of cancer-origin and normal
cells, namely hormone-dependent breast cancer cell line (MCF-7),
non-hormone-dependent breast cancer cell line (MDA-MB-231), ovarian
cancer cell line (Caov-3), cervical cancer cell line (HeLa), liver cancer cell
line (HepG2) and human foreskin fibroblast cell line (Hs27). The
anti-proliferation activities of these extracts were investigated by employing
colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)2,5 diphenyltetrazolium bromide)
assay through time periods of 24, 48 and 72 h. Preliminary results showed that
the methanol extracts had significant effects (p<0.05) on MDA-MB-231
with IC50 values 65.38 μg/mL. The nutritional composition of the
leaves provides a strong basis for emphasizing the nutritional value of Vitex
negundo L.
Keywords: Cancer cell line; MTT assay; proximate
analysis; Vitex negundo L.
ABSTRAK
Kajian mengenai analisis proksimat ke atas daun Vitex negundo L. (VN) dilakukan bagi
mendapatkan data permulaan mengenai sifat perencatan proliferatif terhadap sel
kanser selanjar. Ekstrak akues dan organik daun VN digunakan untuk mengenal
pasti kesan sitotoksik terhadap enam jenis sel kanser dan normal, iaitu sel
selanjar kanser payudara yang bergantung pada hormon (MCF-7), sel selanjar
kanser payudara yang tak bergantung pada hormon (MDA-MB-231), sel selanjar
kanser ovari (Caov-3), sel selanjar kanser serviks (HeLa), sel selanjar kanser
hepar (HepG2) dan sel selanjar fibroblast kulit manusia (Hs27). Aktiviti
anti-proliferatif kedua-dua ekstrak dikaji dengan menggunakan asai MTT (3-(4,5-dimethylthiazol-2-yl)2,5 difeniltetrazolium bromida) melalui tempoh masa 24,
48 dan 72 jam. Data awal menunjukkan ekstrak metanol memberi
kesan signifikan (p<0.05) terhadap
sel MDA-MB-231
dengan nilai IC50 65.38 μg/mL. Komposisi
pemakanan daun ini memberi asas yang kuat bagi menekankan nilai pemakanan yang
ada pada Vitex negundo L
.
Kata kunci: Analisis proksimat; asai MTT;
sel kanser selanjar; Vitex negundo L.
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*Pengarang untuk surat-menyurat; email: rabeta75@yahoo.com
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