Sains Malaysiana 43(5)(2014): 739–744

Phylogenetic Analysis of Respiratory Syncytial Virus Identified at

Universiti Kebangsaan Malaysia Medical Centre

(Analisis Filogenetik Virus Pernafasan Sinsitium di Pusat Perubatan, Universiti Kebangsaan Malaysia)

M.M. RAHMAN*¹, K.K. WONG¹, I. ISAHAK², Z.Z. RASHID¹& H. ALFIZAH¹

¹Department of Microbiology & Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latiff, Cheras, 56000 Kuala Lumpur, Malaysia

²Department of Medical Sciences, Faculty of Medicine and Health Sciences

Universiti Sains Islam Malaysia (USIM), Tingkat 13, Menara B, Persiaran MPAJ

Jalan Pandan Utama, 55100 Kuala Lumpur, Malaysia

Diserahkan: 22 Mei 2012/Diterima: 1 September 2013

ABSTRACT

Human respiratory syncytial virus (RSV) is an important cause of acute respiratory tract infection in infants and young children. Phylogenetic analysis for RSV in Malaysia has not been reported before. We investigated the genetic features of RSV in respiratory specimens from March to August 2011 with molecular methods. From a total of 130 throat swab and nasopharyngeal aspirate specimens, 54 (41.5%) were positive with RSV, identified by in-house real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay. Thirty-four out of 54 (63.0%) RSV positive patients were children below two years old and two (1.4%) were adults. Phylogenetic analysis showed 39 isolates were genotype GA5, 13 genotypes GA2, one genotype GA1 and one genotype GA7. The findings indicated four genotypes of RSV circulating in the country and the predominant genotype is GA5.

Keywords: Genotype GA1, GA2, GA5 and GA7; phylogenetic analysis; respiratory syncytial virus

ABSTRAK

Virus pernafasan sinsitium (RSV) adalah punca utama yang menyebabkan jangkitan saluran pernafasan akut pada bayi dan kanak-kanak. Analisis filogenetik untuk RSV di Malaysia belum pernah dilaporkan. Kami mengkaji ciri-ciri genetik RSV daripada spesimen yang dikumpulkan bermula dari bulan Mac hingga bulan Ogos 2011 dengan cara pengesanan molekular. Daripada jumlah sebanyak 130 spesimen swab tekak dan cecair nasofarink, 54 (41.5%) adalah RSV positif selepas diuji dengan asai dalaman masa nyata transkriptase balik tindak balas berantai polimerase (rRT-PCR). Tiga puluh empat daripada 54 (63.0%) pesakit dengan RSV positif adalah kanak-kanak di bawah dua tahun dan dua (1.4%) adalah dewasa. Analisis filogenetik menunjukkan bahawa 39 virus RSV adalah genotip GA5, 13 genotip GA2, satu genotip GA1 dan satu genotip GA7. Hasil penyelidikan ini menunjukkan empat genotip RSV yang sedang merebak di negara kita dan genotip utama adalah GA5.

Kata kunci: Analisis filogenetik; genotip GA1, GA2, GA5 dan GA7; virus pernafasan sinsitium

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*Pengarang untuk surat-menyurat; email: mmr@ppukm.ukm.edu.my