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Histomorphology of the Lymphoid Tissues of Broiler Chickens
in Kelantan, Malaysia
(Histomorfologi Tisu Limfa
Ayam Pedaging di Kelantan, Malaysia)
MOHD ZAHIRUL ISLAM KHAN1*, MOHD MASUM2, MD. ZUBAYER IBNA KHAN3, ABDUR RAHMAN BIN AZIZ1, MORSHEDA NASRIN2, MOHD NAZMUL HASAN SIDDIQUE2 & MOHD MOKHTAR
BIN ARSHAD1
1Faculty of Veterinary
Medicine, University Malaysia Kelantan, Locked Bag 36, Pengkalan Chepa
16100 Kota Bharu,
Kelantan, Malaysia
2Department of Anatomy
and Histology, Faculty of Veterinary Science, Bangladesh Agricultural
University, Mymensingh-2202, Bangladesh
3Dhaka Medical College,
Dhaka, Bangladesh
Diserahkan: 4 Mac 2013/Diterima:
22 Oktober 2013
ABSTRACT
The present research has been designed
to understand the histomorphological development of lymphatic tissues
of Cobb 500 strains of postnatal broiler chickens, aged between
day old and D14 of
Kelantan, Malaysia by gross and histological study. In the present
study, it was found that the gross weight, length and width of the
thymus, bursa of Fabricius and spleen were increased with the advancement
of ages of the broiler chickens and was found higher from D14 to
D28. Fine septa of connective tissue divide the thymus into lobes
and lobules. The cortex of lobule is densely populated with lymphocytes.
The bursal follicles were composed of a peripheral cortex which
was rich in bursal cells and centrally depopulated medulla. The
mucosal folds of the bursa were lined by pseudostratified columnar
epithelium. The spleen has two compartments, the red and white pulp.
The red pulp consisted principally of red blood cells, while the
majority of the populations of white pulp were the lymphocytes.
The histological mean length and width of the thymic lobules, bursal
follicles and white pulp of the spleen were grown faster with the
advancement of ages at D14 and D28. In conclusion, the increment
of gross and histological parameters of lymphoid organs of broilers
in the present study was due to aging of broilers.
Keywords: Broiler; bursa of
Fabricius; histomorphology; spleen; thymus
ABSTRAK
Kajian dasar dan histologi ini telah
direka khusus untuk memahami pembangunan histomorfologi tisu limfa ayam
pedaging baka Cobb 500 selepas beranak, berumur antara 1 hari hingga 14 hari di
Kelantan, Malaysia. Kajian ini mendapati bahawa berat kasar,
panjang dan lebar timus, bursa fabricius serta limpa telah bertambah dengan
peningkatan umur ayam tersebut dari hari ke 14 ke hari ke 28. Septa halus tisu perantara membahagikan timus kepada cuping dan
lobul. Korteks lobul jelas dipenuhi limfosit. Folikel bursal pula terdiri daripada korteks periferal yang kaya
dengan sel-sel bursal dan mengosongi tengah medula. Lipatan
mukosa bursa dibarisi oleh sel epetelium pseudostratified kolumnar. Limpa mempunyai dua petak, iaitu pulpa merah dan pulpa putih. Pulpa merah terdiri terutamanya daripada sel darah merah,
manakala majoriti pulpa putih dipenuhi limfosit. Purata
panjang dan lebar lobul thimik, folikel bursal dan pulpa putih limpa berkembang
dengan lebih cepat mengikut pertambahan umur. Kesimpulannya, pertambahan
parameter kasar dan histologi organ limfa pada ayam pedaging dalam kajian ini
adalah disebabkan oleh proses penuaan.
Kata kunci: Ayam pedaging; bursa Fabricius;
histomorfologi; limpa; timus
RUJUKAN
Bach, J.F. 1978. Immunology.
5th ed., New York: John Wiley and Sons. pp. 15-35.
Cooper,
M.D., Peterson, R.D.A. & Good, R.A. 1965. Delineation
of the thymic and bursal lymphoid systems in the chicken. Nature 205:
116-143.
Cortan, R.S., Kumar, V.
& Robbin, S.L. 1998. Robbin's Pathogenic Basis of Disease.
4th ed. Philadelphia: W.B. Saunders pp. 163-164. Getty, R. 1975. Sission
and Grossman's The Anatomy of the Domestic Animal. 5th
ed. Vol. 2. Philadelphia: W.B. Saunders Co. pp. 2010-2018.
Gridley, M.F. 1960. Manual of Histologic and Special Staining Technique. New York: McGraw-Hill Book Company, Inc. pp. 28-29, 82-83.
Hodges, R.D. 1974. The Histology of the Fowl. New York: Academic Press.
pp. 35-108.
Honjo, K. & Hirota,
Y. 1993. Immunohistochemical investigations of lymphocytes in the lymphoid
organs of cyclophosphamide treated chickens. Journal of Veterinary Medical
Science 55: 895-897.
Karim, M.R., Khan, M.Z.I. & Haque, Z. 2005. The dynamics
of immunocompetent cells in the major lymphoid organs and mucosa associated lymphoid tissues of
chemotherapy treated chickens. Journal of the Bangladesh Society for
Agricultural Science and Technology 2: 5-8.
Khalil,
Mo., Khan, M.Z.I., Khalil, Ma. & Islam, M.R. 2003. A
prospective study of prenatal and postnatal development of thymus of deshi
chicken. Mymensingh Medical Journal 12: 20-24.
Khalil,
Mo., Khan, M.Z.I., Khalil, Ma. & Islam, M.R. 2002. Effects of age in the
structure of the bursa of Fabricius of deshi chicken of Bangladesh. Mymensingh
Medical Journal 11: 91-93.
Khan,
M.Z.I., Hashimoto, Y. & Asaduzzaman, M. 1998. Development
of T-cell sub-populations in postnatal chicken lymphoid organs. Vetrinarski
Arhiv 68: 183-189.
Khan,
M.Z.I., Hashimoto, Y., Konno, A., Kon, Y. & Iwanaga, T. 1996. Development
of T-lymphocyte subpopulations in the postnatal chicken oviduct. Cell
and Tissue Research 284: 317-325.
Nasrin,
M., Khan, M.Z.I., Siddiqi, M.N.H. & Masum, M.A. 2013. Mobilization of
immunoglobulin (Ig)- containing plasma cells in Harderian gland, cecal tonsil
and trachea of broilers vaccinated with newcastle disease vaccine. Tissue and Cell 45: 191-197.
Rahman, M.L., Islam,
M.R., Asaduzzaman, M. & Khan, M.Z.I. 2003. Lymphoid
tissues in the digestive tract of Deshi Chicken (Gallus domesticus) in
Bangladesh. Pakistan Journal of Biological Science 6: 1145-1150.
*Pengarang untuk surat-menyurat; email: zahirul@umk.edu.my
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