Sains Malaysiana 44(10)(2015): 1453–1459
Microsatellite
DYS385 Polymorphism among the Tai and Mon-Khmer Speaking Populations of
Northern Thailand
(Polimorfisme
Mikrosatelit DYS385 antara Populasi Penutur Tai dan
Mon-Khmer di Utara Thailand)
JATUPOL KAMPUANSAI*
& SIRIWADEE CHOMDEJ
Department of Biology,
Faculty of Science, Chiang Mai University, 50202, Thailand
Diserahkan: 8 Disember
2014/Diterima: 22 Jun 2015
ABSTRACT
Microsatellite DYS385
is a highly polymorphic marker in the Y chromosome. It has been used for
investigating population genetic structure and personal identification in
various ethnic groups of the world. This research aimed to analyze the
microsatellite DYS385 polymorphism among 9 Tai and 11
Mon-Khmer speaking populations of northern Thailand. Fifty-six different
haplotypes were found in 453 samples from 20 populations. Haplotype diversities
and discrimination powers of populations belonging to the Tai linguistic family
was higher than those of the Mon-Khmer group. Genetic affinities based on DYS385
variation do not conform to linguistic classification but a fraction of genetic
divergence patterns can be explained by geographic distances.
Keywords: DYS385;
genetic polymorphism; microsatellite; Mon-Khmer; Tai
ABSTRAK
Mikrosatelit DYS385
adalah petanda polimorfik tertinggi dalam kromosom Y. Ia telah digunakan bagi mengkaji struktur genetik dan pengenalan peribadi dalam
pelbagai kumpulan etnik di dunia. Kajian ini bertujuan untuk
menganalisis polimorfisme mikrosatelit DYS385 antara populasi penutur
9 Tai dan 11 Mon-Khmer di Utara Thailand. Lima
puluh-enam jenis haplo berbeza ditemui dalam 453 sampel daripada 20 populasi. Kepelbagaian jenis haplo dan kuasa diskriminasi populasi
tergolong dalam keluarga linguistik Tai adalah lebih tinggi berbanding kumpulan
Mon-Khmer. Afinisi genetik berdasarkan variasi DYS385
tidak mematuhi klasifikasi linguistik tetapi sebahagian kecil daripada pola
perbezaan genetik dapat dijelaskan oleh jarak geografi.
Kata
kunci: DYS385; Genetik polimorfisme;
mikrosatelit; Mon-Khmer; Tai
RUJUKAN
Besaggio,
D., Fuselli, S., Srikummool, M., Kampuansai, J., Castrì, L., Tyler-Smith, C.,
Seielstad, M., Kangwanpong, D. & Bertorelle, G. 2007. Genetic variation in
Northern Thailand Hill Tribes: Origins and relationships with social structure
and linguistic differences. BMC Evolutionary Biology 7(Suppl 2): S12.
Bhoopat,
T., Hohoff, C. & Steger, H.F. 2003. Identification of
DYS385 allele variants by using shorter amplicons and Northern Thai haplotype
data. Journal of Forensic Science 48(5): 1108-1112.
Dupanloup, I.,
Schneider, S. & Excoffier, L. 2002. A simulated annealing approach to
define the genetic structure of populations. Molecular Ecology 11(12):
2571-2581.
Excoffier, L. &
Lischer, H.L. 2010. Arlequin suite ver 3.5: A new series of programs to perform
population genetics analyses under Linux and Windows. Molecular Ecology
Resource 10: 564-567.
Jobling,
M. & Tyler-Smith, C. 2003. The human Y chromosome: An evolutionary marker
comes of age. Nature review 4: 598-612.
Kampuansai, J., Kutanan,
W., Phuphanitcharoenkul, S. & Kangwanpong, D. 2012. A suggested Khmuic
origin of the hunter-gatherer Mlabri in northern Thailand: Evidence from
maternal DNA lineages. Thai Journal of Genetics 5(2): 203-215.
Kampuansai,
J., Bertoelle, G., Castri, L., Nakbunluang, S., Seielsted, M. &
Kangwanpong, K. 2007. Mitochondrial DNA variation of Tai
speaking people in Northern Thailand. ScienceAsia 33: 443-448.
Kangwanpong,
D., Chaijaruwanich, J., Srikummool, M. & Kampuansai, J. 2004. Selection
of Y-Chromosomal microsatellites for phylogenetic study among Hilltribes in
Northern Thailand using the decision tree induction algorithm. ScienceAsia 30: 239-245.
Kayser, M., Caglia, A.,
Corach, D., Fretwell, N., Gehrig, C., Graziosi, G., Heidorn, F., Herrmann, S.,
Herzog, B., Hidding, M., Honda, K., Jobling, M., Krawczak, M., Leim, K.,
Meuser, S., Meyer, E., Oesterreich, W., Pandya, A., Parson, W., Penacino, G., Perez-Lezaun,
A., Piccinini, A., Prinz, M., Schmitt, C. & Roewer, L. 1997. Evaluation of
Y-chromosomal STRs: A multicenter study. International Journal of Legal
Medicine 110(3): 125-133.
Lewis,
M.P., Gary, F.S. & Charles, D.F. 2009. Ethnologue: Languages of the World, 17th
ed. Dallas, Tex.: SIL International. http://www.ethnologue.com. Access on 13
October 2014.
Pascali, V.L., Dobosz,
M. & Brinkmann, B. 1999. Coordinating Y-chromosomal STR research for the
Courts. International Journal of Legal Medicine 112(1): 1.
Schliesinger, J. 2000. Ethnic
Groups of Thailand: Non-Tai- Speaking Peoples. Bangkok: White Lotus Press.
Seielstad,
M., Bekele, E., Ibrahim, M., Toure, A. & Traore, M. 1999. A
view of modern human origins from Y chromosome microsatellite variation. Genome Research 9: 558-567.
Slatkin, M. 1995. A
measure of population subdivision based on microsatellite allele frequencies. Genetics 139: 457-462.
Willuweit, S. &
Roewer, L. 2007. Y chromosome haplotype reference database (YHRD): Update. Forensic
Science International: Genetics 1(2): 83-87.
*Pengarang untuk surat-menyurat; email: Jatupol.k@cmu.ac.th
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