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Sains Malaysiana 44(8)(2015): 1167–1174 Aqueous Extract of Cornsilk Confers Mild Diuretic Activity in Normal Rats (Ekstrak Akues Sutera
Jagung Cetus Aktiviti
Diuretik Sederhana dalam Tikus Normal) M.A.
SOLIHAH,
A.R.
NURHANAN,
W.A.
WAN
AMIR
NIZAM
& W.I. WAN
ROSLI* Nutrition
Programme, School of Health Sciences,
Universiti Sains Malaysia, 11800
Penang, Malaysia Diserahkan: 1
Jun 2014/Diterima: 1 April 2015 ABSTRACT Cornsilk is traditionally used to treat illnesses related to kidney and as
diuretic agent. The study was performed to evaluate the effectiveness
of Malaysian cornsilk in elevating diuresis
and their dose response relationship in normal Sprague-Dawley
rat. The diuresis activity was determined by administered the
rats with different dose treatments of 400, 500, 600, 700 and 800
mg/kg. Cumulative urine was significantly increased with the dosage
levels (400-600 mg/kg) ranging from 14.06 - 20.13 mL.
Cumulative urine of aqueous extract of cornsilk
(AEC) at 400 mg/kg (14.06 mL) and
500 mg/kg (15.21 mL) treatments found to be significantly lower
than positive control (21.25 mL). In addition, Na+ content
was significantly higher compared with negative control at dosages
of 500, 600, 700 and 800 mg/kg. At any rate, K+ and
Cl- content of all AEC treatments
were not significantly different during 24 h monitoring. The pH
values were increased paralleled with the increment of AEC dosages,
though it was not significant. On the other result, the ED50 of
AEC was observed at 454.10 mg/kg. Malaysian AEC had
shown a mild diuretic activity in elevating urine and Na+ content
at dosages from 500 to 800 mg/kg. Whilst, AEC also
showed an effect of potassium sparing diuretics. Thus, it
is suggested that Malaysian cornsilk can
be used as an alternative natural diuretic agent. Keywords: Aqueous extract;
cornsilk; diuretic property ABSTRAK Sutera jagung kerap
kali digunakan secara
tradisi untuk merawat
masalah kesihatan
yang berkaitan dengan buah pinggang dan
sebagai agen
diuretik. Kajian telah dilakukan untuk menilai keberkesanan
sutera jagung
Malaysia dalam meningkatkan diuresis dan perkaitan respons
dos dalam tikus
Sprague-Dawley normal. Aktiviti diuresis
telah ditentukan
dengan memberi rawatan kepada tikus pada dos yang berbeza (7 kumpulan/6 ekor setiap kumpulan)
iaitu 400, 500, 600, 700 dan
800 mg/kg. Air suling
digunakan sebagai kawalan negatif manakala hidroklorothiazid pula
sebagai kawalan positif. Keputusan kajian menunjukkan
urin kumulatif
meningkat secara signifikan dengan peningkatan dos (400-600 mg/kg) dalam
julat daripada
14.06-20.13 mL. Urin kumulatif ekstrak
akues sutera
jagung (AEC) pada
dos 400 (14.06 mL) dan 500 mg/kg (15.21
mL) didapati lebih rendah daripada kawalan positif (21.25 mL).
Sebagai
tambahan, kandungan Na+ adalah lebih tinggi
secara signifikan
berbanding kawalan negatif pada dos 500, 600, 700 dan 800 mg/kg. Pada semua kadar, kandungan
K+ dan
Cl- bagi
semua perlakuan
AEC
adalah tidak signifikan
dalam masa 24 jam pemerhatian.
Nilai
pH meningkat selari dengan peningkatan dos AEC,
walaupun tidak
signifikan. Dalam keputusan lain, ED50 AEC
adalah sebanyak 454.10 mg/kg. AEC Malaysia telah menunjukkan aktiviti diuretik sederhana dalam meningkatkan kandungan urin dan Na+ pada dos 500 hingga 800 mg/kg.
Sementara
itu, AEC juga menunjukkan
kesan penahanan
diuretik kalium. Oleh itu, sutera jagung
Malaysia dicadangkan boleh
digunakan sebagai agen diuretik semula
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untuk surat-menyurat;
email: rosliishak@gmail.com |
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