Sains Malaysiana 45(6)(2016): 909–914

Effect of Temperature on Lateral Gene Transfer Efficiency of Multi-Antibiotics Resistant Bacterium Alcaligenes faecalis

(Kesan Suhu ke atas Kecekapan Pemindahan Gen Lateral Multi-Antibiotik Rintangan Bakteria Alcaligenes faecalis)

GOUTAM BANERJEE¹*, ARUN KUMAR RAY¹ & RAVI KUMAR²

¹Department of Zoology, Visva-Bharati University, Santinilketan, 731235 West Bengal, India

²Helix Biogenesis, Noida, Sector-2, Pin-201301, Uttar Pradesh, India

Diserahkan: 4 November 2015/Diterima: 28 Disember 2015

ABSTRACT

Antibiotic resistant bacterial population is a great threat for human as well as for other domestic animals. Unscientific use of antibiotics in clinical sectors create a selective pressure on bacteria that make bacteria resistant to a wide range of drugs. The current finding demonstrated the isolation and characterization of multi antibiotics resistant bacterial species from oil contaminated soil and its lateral gene transfer efficiency at different temperature. Among six bacterial isolates, the strain OD1 showed highest antibiotic resistant capacity was selected for further studies. Morphological, biochemical and 16S rDNA sequence analysis confirmed the bacterial strains as Alcaligenes faecalis strain OD1. It was recorded that antibiotics resistant gene transfer was highly dependent on temperature and showed maximum transfer efficiency at 25°C (9.6 ×10-6).

Keywords: Antibiotics resistant bacterium; lateral gene transfer efficiency; temperature; 16S rDNA

ABSTRAK

Populasi antibiotik tahan bakteria adalah ancaman besar bagi manusia dan haiwan domestik lain. Penggunaan antibiotik tidak saintifik dalam sektor klinikal mewujudkan tekanan terpilih pada bakteria yang membuat bakteria tahan kepada pelbagai ubat-ubatan. Penemuan terkini menunjukkan pengasingan dan pencirian pelbagai spesies antibiotik tahan bakteria daripada minyak tanah tercemar dan kecekapan pemindahan gen lateral pada suhu yang berbeza. Antara enam pengasingan bakteria, terikan OD1 menunjukkan kapasiti antibiotik tahan yang tertinggi telah dipilih untuk melanjutkan pelajaran. Analisis morfologi, biokimia dan jujukan 16S rDNA mengesahkan terikan bakteria sebagai ketegangan Alcaligenes faecalis OD1. Ia telah dicatatkan bahawa pemindahan gen antibiotik tahan terlalu bergantung kepada suhu dan menunjukkan kecekapan pemindahan maksimum pada 25°C (9.6 × 10-6).

Kata kunci: Antibiotik rintangan bakteria; kecekapan pemindahan gen lateral; suhu; 16S rDNA

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*Pengarang untuk surat-menyurat, email: banerjee.goutam2@gmail.com