Sains Malaysiana 46(1)(2017): 123–128
http://dx.doi.org/10.17576/jsm-2017-4601-16
Production of Biomass by Schizophyllum
commune and Its Antifungal Activity towards
Rubberwood-Degrading Fungi
(Penghasilan Biojisim oleh Schizophyllum commune
dan Aktiviti Antikulat ke atas Kulat Pereput Kayu Getah)
YI PENG TEOH1,2*, MASHITAH MAT DON1 & SALMIAH UJANG3
1School of Chemical
Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal
Seberang Perai South,
Penang, Malaysia
2Faculty of Engineering
Technology, Universiti Malaysia Perlis (UniMAP),
P.O Box 77,
D/A Pejabat Pos Besar, 01000
Kangar, Perlis Indera Kayangan, Malaysia
3Chemistry and Wood
Protection Programme, Forest Product Division, Forest Research Institute of
Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan, Malaysia
Diserahkan: 18 Jun 2014/Diterima:
29 April 2016
ABSTRACT
Rubberwood is the most popular timber
for furniture manufacturing industry in Malaysia. Major drawback
concerned that rubberwood is very prone to attack by fungi and wood
borers, and the preservation method using boron compounds exhibited
hazardous effect to the workers. Fungal-based biological control
agents have gained wide acceptance and Schizophyllum commune secondary metabolite played an important
role in term of antifungal agent productivity. The effects of initial
pH, incubation temperature and agitation on biomass production by
S. commune were investigated under submerged shake culture.
In this work, it was found that the synthetic medium with initial
solution pH of 6.5 and incubated at 30ºC with shaking at 150
rpm provided the highest biomass production. The biomass extract
from S. commune was then applied onto the rubberwood block
panel to investigate its effectiveness. The results showed that
biomass extract at a concentration of 5 μg/μL could inhibit
the growth of selected rubberwood-degrading fungi, such as Lentinus
sp., L. strigosus and Pycnoporus sanguineus.
Keywords: Antifungal activity;
biomass production; effectiveness; rubberwood-degrading fungi;
Schizophyllum commune
ABSTRAK
Kayu getah
ialah kayu yang paling popular untuk industri pembuatan perabot
di Malaysia. Kelemahan utama bagi kayu getah itu adalah serangan oleh kulat dan
pengorek kayu dan kaedah pengawetan menggunakan sebatian boron menunjukkan
kesan berbahaya kepada pekerja. Agen kawalan biologi yang
berasaskan kulat telah mendapat penerimaan luas, dan metabolit sekunder
Schizophyllum
commune memainkan peranan penting dalam produktiviti agen antikulat.
Kesan pH awal, suhu pengeraman dan penggoncangan bagi penghasilan
biojisim oleh S. commune telah dikaji di bawah kultur
kelalang goncang. Dalam kajian ini, didapati bahawa medium sintetik
dengan pH permulaan 6.5 dan dikultur pada 30ºC dengan goncangan
pada 150 rpm menghasilkan biojisim tertinggi. Ekstrak biojism daripada
S. commune kemudiannya telah ditindakbalaskan ke atas blok
kayu getah untuk menguji akan keberkesanannya. Keputusan menunjukkan
ekstrak biojisim pada kepekatan 5 μg/μL berkeupayaan merencat
pertumbuhan kulat pereput kayu terpilih, seperti Lentinus sp.,
L. strigosus dan Pycnoporus sanguineus.
Kata kunci: Aktiviti antikulat; keberkesanan; kulat pereput kayu getah;
penghasilan biojisim; Schizophyllum commune
RUJUKAN
Adebayo-Tayo, B.C., Jonathan, S.G., Popoola, O.O. & Egbomuche,
R.C. 2011. Optimization of growth conditions for mycelia yield and
exopolysaccharride production by Pleurotus ostreatus cultivated in
Nigeria. African Journal of Microbiology Research 5(15): 2130-2138.
ASTM
D4445. 2003. Standard Test Method for Fungicides for Controlling Sapstain
and Mold on Unseasoned Lumber (Laboratory Method). USA: American Society
for Testing and Material. pp. 1-4.
Bai, Z., Harvey, L.M. & McNeil, B. 2003. Oxidative stress
in submerged cultures of fungi. Critical Reviews in Biotechnology 23(4):
267-302.
Barberel, S.I. &
Walker, J.R.L. 2000. The effect of aeration upon the
secondary metabolism of microorganisms. Biotechnology and Genetic
Engineering Reviews 17(1): 281-323.
Ertola, R.J., Giulietti,
A.M. & Castillo, F.J. 1995. Design, formulation, and
optimization of media. In Asenjo, J.A. & Merchuk, J.C. (Eds.), Bioreactor
System Design. New York: Marcel Dekker Inc. pp. 90-120.
Fasidi, I.O. &
Akwakwa, D.O. 1996. Growth requirements of Volvariella speciosa (Fr. Ex.
Fr.) Sing., a Nigerian mushroom. Food Chemistry 55(2):
165-168.
Fazenda,
M.L., Seviour, R., McNeil, B. & Harvey, L.M. 2008. Submerged culture
fermentation of “higher fungi”: The macrofungi. Advances in Applied
Microbiology 63(1): 33-92.
Gbolagade, J.S., Fasidi,
I.O., Ajayi, E.J. & Sobowale, A.A. 2006. Effect of
physic-chemical factors and semi-synthetic media on vegetative growth of Lentinus
subnudus (Berk.), an edible mushroom from Nigeria. Food Chemistry 99(4): 742-747.
Guo,
X., Zou, X. & Sun, M. 2009. Optimization of a chemically
defined medium for mycelia growth and polysaccharide production by medicinal
mushroom Phellinus igniarius. World Journal of Microbiology
and Biotechnology 25(12): 2187-2193.
Kumari,
M., Survase, S.A. & Singhal, R.S. 2008. Production
of schizophylan using Schizophyllum commune NRCM. Bioresource
Technology 99(5): 1036-1043.
Purwanto, L.A., Ibrahim,
D. & Sudrajat, H. 2009. Effect of agitation speed on morphological changes
in Aspergillus nigerhyphae during production
of tannase. World Journal of Chemistry 4(1): 34-38.
Rau,
U., Gura, E., Oleszewski, E. & Wagner, F. 1992. Enhanced glucan
formation of filamentous fungi by effective mixing, oxygen limitation and
fed-batch processing. Journal of Industrial Microbiology 9(1): 19-25.
Teoh, Y.P., Mashitah,
M.D. & Ujang, S. 2011. Assessment for the properties, utilization, and
preservation of rubberwood (Hevea brasiliensis): A case study in
Malaysia. Journal of Wood Science 57(4): 255-266.
Teoh, Y.P., Mashitah,
M.D. & Ujang, S. 2012. Nutrient improvement using
statistical optimization for growth of Schizophyllum commune, and its
antifungal activity against wood degrading fungi of rubberwood. Biotechnology
Progress 28(1): 232-241.
Zaidon, A., Norhairul,
N.A.M., Faizah, A., Paridah, M.T., Jalaluddin, H., Mohd Nor, M.Y. & Nor
Yuziah, M.Y. 2008. Efficacy of pyrethroid and boron preservatives in protecting particle board against fungus and termite. Journal
of Tropical Forest Science 20(1): 57-65.
Znidarsic, P. &
Pavko, A. 2001. The morphology of filamentous fungi in submerged cultivation as
a bioprocess parameter. Food Technology and Biotechnology 39(3):
237-252.
*Pengarang untuk surat-menyurat; email: ypteoh@unimap.edu.my
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