Sains Malaysiana 46(3)(2017):
439–448
http://dx.doi.org/10.17576/jsm-2017-4603-11
Pencirian Bakteria Asid Laktik dan
Sebatian Aroma Ikan Pekasam
(Characterisation of Lactic Acid Bacteria and Aromatic
Compounds in Fermented
Fish Pekasam)
WEI CHI
TAN, SENG JOE LIM
& WAN AIDA WAN MUSTAPHA*
Pusat Pengajian Sains Kimia dan Teknologi
Makanan, Fakulti Sains dan Teknologi
Universiti Kebangsaan Malaysia, 43600 UKM
Bangi, Selangor Darul Ehsan, Malaysia
Diserahkan: 4 September 2015/Diterima: 16
Jun 2016
ABSTRAK
Dalam kajian ini, bakteria asid laktik
(LAB) serta sebatian aroma ikan pekasam daripada spesies
yang berbeza ditentukan. Persampelan ikan pekasam iaitu tilapia,
loma, lampam, sepat dan gelama diperoleh daripada pembekal Perusahaan
Ikan Pekasam Kiah di Kuala Kangsar, Perak. Penentuan spesies LAB
dijalankan melalui kaedah pencairan bersiri, pengkulturan LAB, ujian katalase, ujian pewarnaan
spora serta ujian pengesanan Gram bakteria dan morfologi. Pengesahan
spesies LAB
dijalankan melalui pengekstrakan asid deoksiribonukleik
(DNA),
amplifikasi dengan tindak balas rantaian polimerasi (PCR),
analisis elektroforesis gel dan penjujukan DNA.
Hasil jujukan DNA yang diperoleh dibandingkan dengan jujukan dalam pangkalan
data GenBank di NCBI menggunakan BLAST.
Didapati Lactobacillus brevis KB290 DNA dan
Lactobacillus casei W56 wujud dalam pekasam tilapia, Lactobacillus
plantarum 16 dalam pekasam lampam, Lactobacillus casei BD-II
kromosom dan Lactobacillus plantarum WCFS1 dalam pekasam sepat,
Corynebacterium vitaeruminis DSM 20294 dan Streptococcus anginosus C1051 dalam
pekasam gelama. Manakala Staphylococcus carnosus subsp. carnosus
TM300
kromosom adalah LAB dominan dalam pekasam loma. Sementara
itu, sebatian aroma ditentukan melalui kaedah pengekstrakan cecair
menggunakan pelarut metanol dan heksana. Pemprofilan sebatian aroma
dijalankan dengan kromatografi gas-spektometer jisim (GC-MS).
Sebatian aroma dalam ekstrak metanol dan heksana daripada lima jenis
ikan pekasam dibandingkan. Bilangan sebatian aroma yang diekstrak
menggunakan metanol adalah lebih banyak berbanding dengan yang menggunakan
heksana. Sebatian aroma yang paling banyak dikesan adalah daripada
pekasam loma. Asid karboksilik merupakan sebatian yang paling dominan
dalam ikan pekasam dan memberi bau hamis serta tengik.
Kata kunci: Bakteria asid laktik;
ikan pekasam; sebatian aroma
ABSTRACT
In this research, lactic acid bacteria
(LAB) and aromatic compounds on different species of pekasam
were determined. The sampling of pekasam which were tilapia, loma,
lampam, sepat and gelama were performed at Kiah Pekasam Enterprise
at Kuala Kangsar, Perak. Several procedures were performed to determine
the LAB,
namely, serial dilution, pour plating, enumeration of LAB,
catalase test, Gram-staining and determination of LAB morphology.
Confirmation of LAB was carried out which were deoxyribonucleic
acid (DNA) extraction, amplification of polymerization
chain reaction (PCR), gel electrophoresis and
DNA
sequencing. The DNA sequence for each strain was matched to the available
sequence from GenBank database via BLAST at NCBI.
Lactobacillus brevis KB290 DNA and
Lactobacillus casei W56 were found on pekasam tilapia, Lactobacillus
plantarum 16 was on pekasam lampam, Lactobacillus casei BD-II
chromosome and Lactobacillus plantarum WCFS1
were on pekasam sepat, Corynebacterium vitaeruminis DSM 20294
and Streptococcus anginosus C1051 were found on pekasam gelama.
Staphylococcus carnosus subsp. carnosus TM300 chromosome was the dominant
LAB on pekasam loma. Liquid extraction was carried out to
determine the aromatic compounds in pekasam, followed by profiling
using gas chromatography-mass spectrometer (GC-MS). Comparison of aromatic
compounds between methanol and hexane extracts from five different
species of pekasam was performed. Our findings indicated that the
methanol extraction was more efficient compared to that of hexane,
as the quantity of aromatic compounds in methanol extract was higher
to those using hexane. The highest amount of aromatic compounds
produced was from pekasam loma. Carboxylic acids were the most dominant
compounds found on pekasam and it gave rancid and 'goaty' off-flavour.
Keywords: Aromatic compounds; asid lactic bacteria; fermented fish
pekasam
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*Pengarang untuk surat-menyurat;
email: wanaidawm@ukm.edu.my
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