Sains Malaysiana 46(4)(2017):
583–588
http://dx.doi.org/10.17576/jsm-2017-4604-10
Kajian Serologi Toksoplasmosis
dalam Kalangan Penerima Transplan Renal di Hospital Kuala Lumpur
(Serological Study of
Toxoplasmosis among Renal Transplant Recipients in Kuala Lumpur
Hospital)
HARTINI
YUSOF1
& MOHAMED KAMEL ABD
GHANI2*
1Jabatan Teknologi Makmal
Perubatan, Fakulti Sains Kesihatan, Universiti Teknologi MARA
Kampus Puncak Alam, 42300
Puncak Alam, Selangor Darul Ehsan, Malaysia
2Jabatan Sains Bioperubatan,
Fakulti Sains Kesihatan Bersekutu, Universiti Kebangsaan Malaysia
50300 Jalan Raja Muda
Abdul Aziz, Kuala Lumpur, Wilayah Persekutuan, Malaysia
Diserahkan: 16 Jun 2016/Diterima:
10 Oktober 2016
ABSTRAK
Toksoplasmosis adalah
penyakit akibat daripada jangkitan Toxoplasma gondii kerana
protozoa ini merupakan patogen oportunistik yang penting dalam kalangan
penerima organ. Jangkitan biasanya adalah tanpa gejala tetapi ia
boleh menjadi serius sehingga mengancam nyawa terutama bagi pesakit
terimunokompromi. Oleh itu, suatu kajian irisan lintang dengan pensampelan
purposif telah dijalankan untuk mengesan kadar prevalens jangkitan
T. gondii dalam kalangan 171 orang pesakit renal kronik di
Hospital Kuala Lumpur (HKL).
Subjek kajian dibahagi kepada 2 kumpulan iaitu kumpulan penerima
transplan renal yang terdiri daripada 138 orang pesakit renal kronik
dan kumpulan kawalan yang merupakan 33 orang pesakit renal kronik
yang belum menjalani transplantasi renal. Kaedah serologi iaitu
‘Indirect Immunofluorescent Antibody’ (IFA) digunakan untuk mengesan IgG anti-Toksoplasma di dalam
serum kedua-dua kumpulan pesakit. Selain itu, kadar seropositif
dan min titer IgG ditentukan berdasarkan tempoh pascatransplantasi.
Sebanyak 39.4% pesakit daripada kumpulan kawalan dikesan mempunyai
antibodi IgG terhadap T. gondii manakala kadar seropositif
dalam kalangan pesakit pascatransplan adalah 30.4%. Hasil kajian
turut menunjukkan tempoh pascatransplantasi tidak mempengaruhi corak
jangkitan T. gondii walaupun terdapat peningkatan kadar seropositif
jangkitan selepas 6 bulan transplantasi renal dijalankan. Di samping
itu, wujudnya korelasi antara min titer IgG dan tempoh pascatransplantasi
dengan titer meningkat secara signifikan daripada 2.68 kepada 7.14
sejajar dengan pertambahan tempoh pascatransplantasi renal. Kadar
kes toksoplasmosis dalam kalangan pesakit pascatransplan boleh dikurangkan
sekiranya ujian saringan awal terhadap jangkitan T. gondii dijalankan.
Profilaksis dan pemeriksaan susulan juga perlu dilakukan ke atas
pesakit renal kronik untuk mengelak daripada berlakunya sebarang
kerumitan yang teruk.
Kata kunci: Renal; serologi;
transplantasi; toksoplasmosis
ABSTRACT
Toxoplasmosis is a parasitic
disease caused by an important opportunistic pathogen, Toxoplasma gondii. Infection with T. gondii is often asymptomatic
but it can be lethal in immunocompromised patients. Therefore, a
cross-sectional study with purposive sampling had been conducted
in order to determine the prevalence of T. gondii infection
in 171 patients with chronic renal diseases. The patients were divided
into 2 groups: 138 of chronic renal patients who received renal
transplants were categorized in renal transplant recipient group
and 33 patients of chronic renal disorders who have not yet received
renal transplants were selected as a control group. The serological
Indirect Fluorescent Antibody Test (IFAT)
was used to detect the presence of IgG anti-Toxoplasma in sera from
both groups. This study showed that the prevalence of T. gondii
infection in control and renal transplant patients were 39.4%
and 30.4%, respectively. Furthermore, there was no significant association
between post-transplantation period and pattern in prevalence of
T. gondii infection, however, it was noted that the prevalence
rates of T. gondii had increased after 6 months post transplantation.
In addition to that, the significant correlation occurring between
mean titer of IgG where the values increased from 2.68 to 7.14 with
the duration of renal postransplantation. Therefore, the prevalence
of infection among renal transplant patients may reduce if screening
tests are not being neglected. Patients with chronic renal disease
should also be administered with prophylaxis and followed up for
toxoplasmosis to prevent severe complications after transplantation
procedure.
Keywords: Renal; serology; toxoplasmosis; transplantation
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*Pengarang
untuk surat-menyurat; email: profkamel@ukm.edu.my
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