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Sains Malaysiana 47(8)(2018): 1787–1794 http://dx.doi.org/10.17576/jsm-2018-4708-17
Physicochemical
and Structural Characterization of Surface Modified Electrospun
PMMA Nanofibre (Pencirian
Fizikokimia dan Struktur bagi Permukaan Termodifikasi Elektroputaran
Nanogentian PMMA)
RABIATUL ADAWIYAH RAZALI1, YOGESWARAN LOKANATHAN1, SHIPLU ROY CHOWDHURY1, AMINUDDIN SAIM2 & RUSZYMAH HAJI IDRUS1,3*
1Tissue Engineering Centre, Universiti Kebangsaan Malaysia (UKM)
Medical Centre, Jalan Yaa'cob Latiff, 56000 Cheras, Kuala Lumpur,
Federal Territory, Malaysia
2Ear, Nose and Throat Consultant Clinic, KPJ Ampang Puteri
Specialist Hospital, Jalan Mamanda 9, Taman Dato Ahmad Razali, 68000 Ampang,
Selangor Darul Ehsan, Malaysia
Diserahkan: 8 Januari 2018/Diterima: 19
April 2018
ABSTRACT
Although electrospun poly(methyl methacrylate)
(PMMA) may mimic structural features of
extracellular matrix, its highly hydrophobic nature causes reduced
cell attachment. This study analysed the physicochemical and structural
changes of the surface modified PMMA nanofiber. The electrospun PMMA
nanofibers (PM) were surface-treated as follows:
PM
alone, collagen coated-PM (PM-C),
UV-irradiated
PM
(PM-UV), collagen coated UV-irradiated
PM
(PM-C-UV) and collagen coated-PM
crosslinked with genipin (PM-C-GEN). They were subjected to scanning electron microscopy,
Fourier transform infrared (FTIR), cell attachment analysis,
X-ray photoelectron spectroscopy (XPS),
atomic force microscopy and X-ray powder diffraction (XRD).
The surface roughness was lower in PM-C-UV group compared to others.
Based on FTIR results, all expected functional
group were present in all groups. XPS result showed that there are
changes in the mass concentration of UV-treated surfaces and in the
collagen coated surfaces. All PM groups showed amorphous nature
through XRD. UV irradiation
and collagen coating were shown to increase PM’s
functional groups and modify its surface, which contributed to the
increased attachment of cells onto the inert PM scaffold. As conclusion, collagen
coated UV irradiated PMMA provided
a better surface for cell to attach hence are suitable to be used
further as scaffold for in vitro model.
Keywords: Electrospun nanofiber; PMMA;
scaffold; surface modification; UV irradiation
ABSTRAK
Walaupun elektroputaran poli(metil metakrilat) (PMMA)
boleh memimik sifat struktur matriks ekstrasel, ia terlalu hidrofobik
lantas menyebabkan pengurangan pelekatan sel. Kajian ini telah menganalisis
perubahan fizikokimia dan strukur permukaan termodifikasi nanogentian
PMMA.
Permukaan terawat nanogentian PMMA (PM)
yang telah dielektroputar terbahagi seperti berikut: PM sahaja,
PM
bersalut kolagen (PM-C), PM diradiasi
dengan UV bersalut kolagen (PM-UV),
dan PM bersalut kolagen disilang dengan genipin (PM-C-GEN).
Antara analisis yang dijalankan adalah mikroskopi elektron penskanan
(SEM),
inframerah transformasi Fourier (FTIR), analisis pelekatan sel,
spektroskopi fotoelektron sinar-X (XPS), mikroskopi daya atom (AFM)
dan pembelauan sinar-X (XRD). Kekasaran permukaan kumpulan PM-C-UV
adalah kurang berbanding dengan yang lain. Berdasarkan
hasil FTIR,
semua kumpulan berfungsi yang dijangka wujud dalam semua kumpulan.
Keputusan XPS
menunjukkan bahawa terdapat perubahan dalam kepekatan
jisim pada permukaan yang telah di UV
radiasi dan yang telah disalut kolagen. XRD analisis menunjukkan semua
kumpulan PM mempunyai sifat amorf. Sinar UV dan
salutan kolagen telah menyebabkan peningkatan di dalam kumpulan
berfungsi PM lantas mengubah suai permukaannya dan menyebabkan peningkatan
pelekatan sel dalam perancah PM yang lengai. Kesimpulannya, PMMA
diradiasi dengan UV bersalut kolagen adalah permukaan
yang lebih baik untuk pelekatan sel dan menjadikannya sesuai untuk
digunakan sebagai perancah model in vitro.
Kata kunci: Elektroputaran nanogentian; PMMA; perancah; permukaan terubah; sinar UV
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*Pengarang untuk surat-menyurat; email: ruszyidrus@gmail.com
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