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Sains Malaysiana
49(11)(2020): 2793-2800
http://dx.doi.org/10.17576/jsm-2020-4911-18
Green Tea (Camellia
sinensis) Extract Reduces Peptic Ulcer Induced by Helicobacter pylori in Sprague Dawley Rats
(Ekstrak Teh Hijau (Camellia sinensis) Mengurangkan Ulser Peptik yang Diaruh oleh Helicobacter pylori pada Tikus Sprague
Dawley)
1Drug and Herbal Research Centre, Faculty
of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Federal
Territory, Malaysia
2The Center of Quality Management of
Medicine, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala
Lumpur, Federal Territory, Malaysia
Diserahkan: 26 November 2019/Diterima: 16 Mei 2020
ABSTRACT
The
study was conducted to evaluate the anti-peptic ulcer activity of ethanol
extract of Camellia
sinensis (green tea) induced by Helicobacter
pylori in Sprague Dawley rats. The standard regimen of anti-peptic ulcer
agents, clarithromycin 30 mg/kg, amoxicillin 30 mg/kg and esomeprazole 3 mg/kg
used as the positive control. The rats divided into 6 groups (n = 6), normal
control, vehicle control (2% of tragacanth
suspension), positive control for preventive and treatment groups, preventive
and treatment groups were given 50 mg/kg orally of green tea extract for 14
days before and after inoculation (as prevention and treatment, respectively)
with 5×108 CFU/mL H. pylori suspension orally twice daily for
7 days. Anti-peptic ulcer activity was evaluated based on the pH of gastric
juice, ulcer score and body weight of rats. The results demonstrated that the
use of green tea for prevention or treatment showed a significant difference
(p<0.001) in reducing the pH of gastric juice 3.88 ± 0.09 and 5.74 ± 0.08,
respectively, compared to vehicle control 5.85 ± 0.12. Meanwhile, vehicle
control rats had high significantly reduced body weight when compared with all groups
treated. The preventive and the treatment groups showed significantly reduce in
ulcer score to 0.50 ± 0.18 (p<0.01) and 0.83 ± 0.10 (p<0.05),
respectively, compared to vehicle control 1.50 ± 0.22. Meanwhile, it was
comparable to the positive control. In conclusion, H. pylori suspension 5×108 CFU/mL given orally twice
daily for seven days able to induce ulcer in Sprague Dawley rats, and the green
tea extract 50 mg/kg was comparable to the standard regimen as anti-peptic
ulcer agent.
Keywords: Anti-peptic ulcer; Camellia sinensis; green tea extract; Helicobacter
pylori; ulcer score
ABSTRAK
Kajian
ini dijalankan untuk menilai aktiviti anti-ulser peptik ekstrak etanol Camellia sinensis (teh hijau)
yang diaruhkan oleh Helicobacter pylori pada tikus Sprague Dawley.
Regimen piawai agen anti-ulser peptik, klaritromisin 30 mg/kg, amoksisilin 30
mg/kg dan esomeprazol 3 mg/kg digunakan sebagai kawalan positif. Tikus
dibahagikan kepada 6 kumpulan (n = 6), kawalan normal, kawalan pembawa (2% suspensi tragacanth), kawalan positif untuk
kumpulan pencegahan dan rawatan, kumpulan pencegahan dan rawatan yang diberikan
ekstrak teh hijau 50 mg/kg secara oral selama 14 hari sebelum atau selepas
inokulasi (sebagai kumpulan pencegahan dan rawatan) berserta dengan pemberian
ampaian 5×108 CFU/mL H. pylori secara oral sebanyak dua kali
sehari selama 7 hari. Aktiviti anti-ulser peptik dinilai berdasarkan pH jus
gastrik, skor ulser dan berat badan tikus. Keputusan menunjukkan bahawa
penggunaan teh hijau untuk pencegahan atau rawatan menunjukkan perbezaan yang
signifikan (p<0.001) dalam mengurangkan pH jus gastrik 3.88 ± 0.09 dan 5.74
± 0.08, setiap satu, berbanding dengan kawalan pembawa 5.85 ± 0.12. Sementara
itu, terdapat penurunan berat badan yang ketara dalam tikus kawalan pembawa
apabila dibandingkan dengan semua kumpulan yang dirawat. Kumpulan pencegahan
dan rawatan menunjukkan penurunan skor ulser menjadi 0.50 ± 0.18 (p<0.01)
dan 0.83 ± 0.10 (p<0.05), berbanding kawalan pembawa 1.50 ± 0.22. Ia adalah
setanding dengan kawalan positif. Sebagai kesimpulan, pemberian ampaian H. pylori 5×108 CFU/mL diberikan secara oral dua kali setiap hari selama tujuh hari dapat
menyebabkan ulser pada tikus Sprague Dawley dan ekstrak teh hijau 50 mg/kg
adalah setanding dengan regimen piawai sebagai agen anti-ulser peptik.
Kata kunci: Anti-ulser peptik; Camellia sinensis; ekstrak teh
hijau; Helicobacter pylori; skor ulser
RUJUKAN
Adhikary,
B., Yadav, S.K., Roy, K., Bandyopadhyay, S.K. & Chattopadhyay, S. 2011.
Black Tea and theaflavins assist healing of indomethacin-induced gastric
ulceration in mice by antioxidative action. Evidence-Based Complementary and
Alternative Medicine 2011: Article ID. 546560.
Adinortey,
M.B., Ansah, C., Galyuon, I. & Nyarko, A. 2013. In vivo models used
for evaluation of potential antigastroduodenal ulcer agents. Ulcers 13(2): 1-13.
Amić,
D., Davidović-Amić, D., Bešlo, D. & Trinajstić, N. 2003.
Structure-radical scavenging activity relationships of flavonoids. Croatica
Chemica Acta 76(1): 55-61.
Azmi, N.,
Chee, S.H., Mohd Fauzi, N., Jasamai, M. & Kumolosasi, E. 2018. Viability
and apoptotic effects of green tea (Camellia sinensis) methanol extract
on human leukemic cell lines. Acta Poloniae Pharmaceutica 75(1): 51-58.
Burkitt,
M.D., Duckworth, C.A., Jonathan, M., Williams, J.M. & Pritchard, D.M. 2017. Helicobacter pylori-induced gastric
pathology: Insights from in vivo and ex vivo models. Disease
Models & Mechanisms 10: 89-104.
Dunne,
C., Dolan, B. & Clyne, M. 2014. Factors that mediate colonization of the
human stomach by Helicobacter pylori. World Journal of
Gastroenterology 20(19): 5610-5624.
Elseweidy,
M.M., Taha, M.M., Younis, N.N., Ibrahim, K.S., Hamouda, H.A., Eldosouky, M.A.
& Soliman, H. 2010. Gastritis induced by Helicobacter pylori infection in experimental rats. Digestive Diseases and Sciences 55(10):
2770-2777.
Hirayama,
F., Takagi, S., Yokoyama, Y., Iwao, E. & Ikeda, Y. 1996. Establishment of
gastric Helicobacter pylori infection in mongolian gerbils. The
Journal of Gastroenterology 31(9): 24-28.
Mabe, K.,
Yamada, M., Oguni, I. & Takahashi, T. 1999. In vitro and in vivo activities of tea catechins
against Helicobacter pylori. Antimicrobial Agents and Chemotherapy 43(7):
1788-1791.
Mahmood,
A.A., Mariod, A.A., Al-Bayaty, F. & Abdel-Wahab, S.I. 2009. Antiulcerogenic
activity of Gynura procumbens leaf extract against
experimentally-induced gastric lesions in rats. Journal of. Medicinal Plants
Research 4(8): 685-691.
Mégraud,
F. 2012. The challenge of Helicobacter pylori resistance to antibiotics:
The comeback of bismuth-based quadruple therapy. Therapeutic Advances in
Gastroenterology 5(2): 103-109.
Nair, V.,
Arjuman, A., Gopalakrishna, H.N., Dorababu, P., Mirshad, P.V., Bhargavan, D.
& Chatterji, D. 2010. Evaluation of the anti-ulcer activity of NR-ANX-C (a
polyherbal formulation) in aspirin & pyloric ligature induced gastric
ulcers in albino rats. The Indian Journal of Medical Research 132:
218-223.
Palframan,
S.L., Kwok, T. & Gabriel, K. 2012. Vacuolating cytotoxin A (VacA), a key
toxin for Helicobacter pylori pathogenesis. Frontiers in Cellular and
Infection Microbiology 2(92): 1-9.
Prabha,
P., Karpagam, T., Badrinarayanan, V. & Packiavathy, A.S.C. 2011. Indigenous
anti-ulcer activity of Musa sapientum on peptic ulcer. Pharmacognosy
Research 3(4): 232-238.
Savoldi,
A., Carrara, E., Graham, D.Y., Conti, M. & Tacconelli, E. 2018. Prevalence
of antibiotic resistance in Helicobacter
pylori: A systematic review and meta-analysis in world health organization
regions. Gastroenterology 155(5): 1372-1382.
Shahamat,
M., Mai, U.E.H., Paszko-Kolva, C., Yamamoto, H. & Colwell, R.R. 1991.
Evaluation of liquid media for growth of Helicobacter pylori. Journal
of Clinical Microbiology 29: 2835-2837.
Sonwjaya,
K., Spandana, U., Manjula, R.R., Havilah, E.S., Sravani, T. & Rao, G.S.N.
2016. Wound healing activity and
anti-ulcer activity of ethanolic extract of peels of Annas Comosus. European
Journal of Pharmaceutical and Medical Research 3(3): 417-422.
Tombola,
F., Campello, S., De Luca, L., Ruggiero, P., Del Giudice, G., Papini, E. &
Zoratti, M. 2003. Plant polyphenols inhibit vaca, a toxin secreted by the
gastric pathogen Helicobacter pylori. FEBS Letters 543: 184-189.
Werawatganon,
D. 2014. Simple animal model of Helicobacter pylori infection. World
Journal of Gastroenterology 20(21): 6420-6424.
Xia,
H.X., English, L., Keane, C.T. & O’Morain, C.A. 1993. Enhanced cultivation
of Helicobacter pylori in liquid media. Journal of Clinical Pathology 46: 750-753.
Xu, J.,
Czinn, S.J. & Blanchard, T.G. 2010. Maintenance of Helicobacter pylori cultures in agar stabs. Helicobacter 15(5): 477-480.
*Pengarang untuk
surat-menyurat; email: e_kumolosasi@ukm.edu.my
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