Sains Malaysiana 50(6)(2021): 1589-1598

http://doi.org/10.17576/jsm-2021-5006-07

Antifungal Activity of Aqueous Plant Extracts and Effects on Morphological and Germination of Fusarium Fruit Rot Pathogens

(Aktiviti Antikulat Ekstrak Tumbuhan Akueos dan Kesannya kepada Morfologi dan Percambahan oleh Patogen Reput Buah Fusarium)

NUR BAITI ABD MURAD¹, MUSKHAZLI MUSTAFA¹, KHOZIRAH SHAARI² & NUR AIN IZZATI MOHD ZAINUDIN¹*

¹Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

²Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

Diserahkan: 22 Jun 2020/Diterima: 31 Oktober 2020

ABSTRACT

Fusarium fruit rot caused by Fusarium species pathogens can be considered a threat to economic loss. The use of synthetic antifungals to control the pathogens has failed to the development of resistance of pathogens. Previous studies reported that plant extracts may contain various bioactive constituents that are able to control pathogen growth. Hence, the aims of this study are to examine the inhibition activity of some plant fresh extracts on mycelial growth and morphological changes of Fusarium oxysporum, F. proliferatum, and F. solani, causal agents of Fusarium fruit rot. Aqueous extract of selected plants was evaluated for their inhibition activity against all the three Fusarium pathogens under in vitro condition using poisoned food bioassay. Averrhoa bilimbi fruit extract demonstrated a highly significant effect against mycelial growth of the pathogens with fungal inhibition percentage of 80.51% for F. oxysporum, 61.28% for F. proliferatum and 58.97% for F. solani. The results showed that the highest formation of 100% extract has lowered the conidia concentration and germination percentage of F. oxysporum (35.43%), F. proliferatum (47.61%), and F. solani (38.67%) compared to the control. Significant shriveled mycelia were observed via scanning electron microscope (SEM) on the pathogens treated with A. bilimbi extract, indicating morphological changes occurred in the cell membrane compared to the control in which the mycelia were in normal form. This innovation, which can be prepared and applied at home, has the potential as an eco-friendly and a benign approach to control fruit rot pathogen.

Keywords: Antifungal activity; Fusarium species; in vitro; plant extracts; SEM

ABSTRAK

Penyakit Fusarium reput buah oleh jangkitan patogen spesiesFusarium mengakibatkan kerugian dalam sektor ekonomi. Kekerapan penggunaan racun sintetik antikulat bagi mengawal patogen menyebabkan patogen menjadi lebih rintang. Kajian terdahulu mendapati bahawa ekstrak tumbuhan mempunyai pelbagai kandungan bahan bioaktif yang mampu mengawal pertumbuhan patogen pada hasil tanaman. Oleh itu, matlamat kajian ini adalah untuk mengkaji aktiviti perencatan oleh beberapa ekstrak segar tumbuhan terhadap pertumbuhan miselium dan perubahan morfologi patogen yang menyebabkan penyakit Fusarium reput buah seperti Fusarium oxysporum, F. proliferatum dan F. solani. Ekstrak akueos daripada beberapa tumbuhan terpilih dinilai daripada segi kesan aktiviti perencatannya ke atas ketiga-tiga patogen spesies Fusarium secara in vitro dengan menggunakan kaedah bioassai makanan beracun. Ekstrak buah Averrhoa bilimbi (bilimbing buluh) menunjukkan kesan yang amat ketara dalam mengawal pertumbuhan miselium patogen dengan peratusan perencatan sebanyak 80.51% bagi F. oxysporum, 61.28% bagi F. proliferatum dan 58.97% bagi F. solani. Hasil kajian mendapati kepekatan ekstrak tertinggi sebanyak 100% memberi kesan dalam penghasilan dan percambahan spora patogen yang rendah, iaitu hanya 35.43% bagiF. oxysporum, 47.61% bagi F. proliferatum dan 38.67% bagiF. solani apabila dibandingkan dengan patogen kawalan masing-masing. Pengecutan miselium yang ketara ke atas patogen yang diberikan rawatan ekstrak buah belimbing buluh dapat diperhatikan melalui SEM yang menunjukkan bahawa terdapat perubahan morfologi pada sel membran jika dibandingkan dengan patogen kawalan yang masih dalam keadaan normal. Inovasi yang dapat dihasilkan dan diguna pakai di rumah ini mempunyai potensi sebagai salah satu cara pengawalan patogen penyakit reput buah yang mesra alam dan menjimatkan kos.

Kata kunci: Aktiviti antikulat; ekstrak tumbuhan; in vitro; SEM; spesies Fusarium

RUJUKAN

Abd Murad, N.B., Mohamed Nor, N.M.I., Shohaimi, S. & Mohd Zainudin, N.A.I. 2017. Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia. Journal of Applied Microbiology 123(6): 1533-1546.

Abu Bakar, A.I., Nur Ain Izzati, M.Z. & Umi Kalsom, Y. 2013. Diversity of Fusarium species associated with post-harvest fruit rot disease of tomato. Sains Malaysiana 42(7): 911-920.

Alhassan, A.M. & Ahmed, Q.U. 2016. Averrhoa bilimbi Linn.: A review of its ethnomedicinal uses, phytochemistry, and pharmacology. Journal of Pharmacy & Bioallied Science 8(4): 265-271.

Asma, A. 2019. Characterization of Fusarium species associated with wilt disease of cucurbit and effectiveness of Trichoderma asperellum as biological enhancer. Universiti Putra Malaysia. Ph.D. Thesis (Unpublished).

Aziz, M.A., Rahman, S., Islam, M. & Begum, A.A. 2014. A comparative study on antibacterial activities and cytotoxic properties of various leaves extracts of Averrhoa bilimbi. International Journal of Pharmaceutical Sciences and Research 5(3): 913-918.

Aziz, N.A. 2016. A review of the antimicrobial properties of three selected underutilized fruits of Malaysia. International Journal of Pharmaceutical and Clinical Research 8(9): 1278-1283.

Breda, C.A., Gasperini, A.M., Garcia, V.L., Monteiro, K.M., Bataglion, G.A., Eberlin, M.N. & Duarte, M.C.T. 2016. Phytochemical analysis and antifungal activity of extracts from leaves and fruit residues of Brazilian Savanna plants, aiming its use as safe fungicides. Natural Product and Bioprospect 6(4): 195-204.

Carandang, V.C.A., Hernandez, M.R.V. & Alvaira, C.E. 2017. In vitro screening of anti-fungal effect of Averrhoa bilimbi leaves methanolic extract against Microsporum canis. Lyceum of the Philippines-St. Cabrini College of Allied Medicine Research 2(2): 58-66.

da Cruz Cabral, L., Pinto, V.F. & Patriarca, A. 2013. Application of plant derived compounds to control fungal spoilage and mycotoxin production in foods. International Journal of Food Microbiology 166(1): 1-14.

de Lima, V.L.A.G., Mélo, E.D.A. & Lima, L.D.S. 2001. Physicochemical characteristics of bilimbi (Averrhoa bilimbi L.). Revista Brasileira de Fruiticultura 23(2): 421-423.

Dey, U., Harlapur, S.I., Dhutraj, D.N., Suryawanshi, A.P., Jagtap, J.P., Apet, K.T., Badgujar, S.L., Gholve, V.M., Kamble, H.N., Kuldhar, D.P. & Wagh, S.S. 2013. Effect of fungicides, botanicals, bioagents and Indigenous Technology Knowledge (ITK’s) on germination of urediniospores of Puccinia sorghi in vitro. African Journal of Agricultural Research 8(39): 4960-4971.

Elsherbiny, E.A., Amin, B.H. & Baka, Z.A. 2016. Efficiency of pomegranate (Punica granatum L.) peels extract as a high potential natural tool towards Fusarium dry rot on potato tubers. Postharvest Biology and Technology 111: 256-263. 

Gholamnezhad, J. 2019. Effect of plant extracts on activity of some defense enzymes of apple fruit in interaction with Botrytis cinerea. Journal of Integrative Agriculture 18(1): 115-123.

Gumgumjee, N.M., Khedr, A. & Hajar, A.S. 2012. Antimicrobial activities and chemical properties of Tamarindus indica L. leaves extract. African Journal of Microbiology Research 6(32): 6172-6181.

Hassan, R., El-Kadi, S. & Sand, M. 2015. Effect of some organic acids on some fungal growth and their toxins production. International Journal of Advances in Biology 2(1): 1-11.

Ma, L.J., Geiser, D.M., Proctor, R.H., Rooney, A.P., O’Donnell, K., Trail, F., Gardiner, D.M., Manners, J.M. & Kazan, K. 2013. Fusarium pathogenomics. Annual Review of Microbiology 67: 399-416.

Mdee, L.K., Masoko, P. & Eloff, J.N. 2009. The activity of extracts of seven common invasive plant species on fungal phytopathogens. South African Journal of Botany 75(2): 375-379.

Mondali, N., Mojumdar, A., Chatterje, S., Banerjee, A., Datta, J.K. & Gupta, S. 2009. Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium. Journal Application Science Environment Management 13(1): 49-53.

Nakyinsige, K., Abdul Rahman, N.S., Salwani, M.S., Abd Hamid, A., Adeyemi, K.D., Sakimin, S.Z. & Sazili, A.Q. 2016. Effect of belimbing buluh (Averrhoa bilimbi) juice extract on oxidative stability and microbiological quality of spent chicken meat. International Food Research Journal 23(6): 2675-2680.

Nur Fatimma, A., Munirah, M.S., Sharifah Siti Maryam, S.A.R., Najihah, A. & Nur Ain Izzati, M.Z. 2018. Efficacy of Allium sativum extract as post-harvest treatment of fruit rot of mango. Plant Pathology and Quarantine 8(2): 144-152.

Rana, S.M.M., Billah, M.M., Hossain, M.S., Saifuddin, A.K.M., Islam, S.K.M.A., Banik, S., Naim, Z. & Raju, G.S. 2014. Susceptibility of microorganism to selected medicinal plants in Bangladesh. Asian Pacific Journal of Tropical Biomedicine 4(11): 911-917.

Raut, J.S. & Karuppayil, S.M. 2014. A status review on the medicinal properties of essential oils. Industrial Crops and Products 62: 250-264.

Serpa, R., França, E.J.G., Furlaneto-Maia, L., Andrade, C.G.T.J., Diniz, A. & Furlaneto, M.C. 2012. In vitro antifungal activity of the flavonoid baicalein against Candida species. Journal of Medical Microbiology 61(Pt 12): 1704-1708.

Shuping, D.S.S. & Elof, J.N. 2017. The use of plants to protect plants and food against fungal pathogens: A review. African Journal of Traditional Complement Alternative Medicines 14(4): 120-127.

Zakaria, L. 2017. Mycotoxigenic Fusarium species from agricultural crops in Malaysia. JSM Mycotoxins 67(2): 67-75.

Zakaria, L., Chik, M.W., Heng, K.W. & Salleh, B. 2012. Fusarium species associated with fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava (Psidium guajava). Malaysian Journal of Microbiology 8(2): 127-130.

Zulhaimi, H.I., Rosli, I.R., Kasim, K.F., Akmal, H.M., Nuradibah, M.A. & Sam, S.T. 2017. A comparative study of Averrhoa bilimbi extraction method. In AIP Conference Proceedings. AIP Publishing LLC. 1885(1): 020178.

*Pengarang untuk surat-menyurat; email: ainizzati@upm.edu.my