Sains
Malaysiana 50(8)(2021): 2141-2152
http://doi.org/10.17576/jsm-2021-5008-01
Effects of
Prohexadione Calcium Applications on Growth and Yield Characteristics of Cucumber (Cucumis sativus L.)
(Kesan Penggunaan
Kalsium Proheksadion kepada Ciri Pertumbuhan dan Hasil Timun (Cucumis sativus L.)
HAKAN BAŞAK*
Kırşehir Ahi Evran University, Faculty of Agriculture, Department of
Horticulture, Turkey
Diserahkan: 8 Ogos 2020/Diterima: 19 Disember 2020
ABSTRACT
This study was conducted to determine the effects of growth regulator
prohexadione calcium (Pro-Ca) and inhibitor gibberellic acid on some parameters
(yield, pigment levels of leaves, nutrient contents, proline, enzyme activities
and fruit quality) when applied at different doses (0, 15, 30, 45, and 60 mg L-1).
Chemicals were applied after transplanting seedlings. A total of 12 plots
including 3 plants for each dose were used in randomised plot experimental
design. Although it has a positive effect on leaf proline content and enzyme
activities, 45, and 60 mg L-1 doses of Pro-Ca had negative effects on morphological parameters (excluding
stem diameter), yield, leaf pigment (excluding chlorophyll a)
and nutrient contents compared to control. High Pro-Ca doses generally affected
the fruit quality properties positively while 15 mg L-1 of Pro-Ca
did not affect other parameters, except decreasing fruit yield. A 30 mg L-1 of Pro-Ca dose was the most positive effective dose on the examined parameters.
The highest vegetative development, yield, nutrient uptake and proline content
were obtained from the plants subjected to 30 mg L-1 of Pro-Ca dose.
Moreover, this dose increased fruit firmness, TSS, EC values and enzyme
activities compared to control. Being an important quality criterion in
cucumber storage after harvest, the weight loss rate 29.4% was lower in fruits
obtained from the plant having 30 mg L-1 of Pro-Ca dose compared to
that of the control plants. Therefore, 30 mg L-1 of Pro-Ca
application, beside its positive effects on plant growth, yield and fruit
quality, can be suggested for also increase shelf life of fruits in cucumber
cultivation in greenhouse.
Keywords: Cucumber; fruit quality; growth; prohexadione
calcium; yield
ABSTRAK
Penyelidikan ini dijalankan untuk mengkaji kesan penggunaan pengatur pertumbuhan kalsium proheksadion (Pro-Ca)
dan perencat asid giberelik pada beberapa parameter
(hasil, tahap pigmen daun-daun, kandungan nutrien, prolina, aktiviti enzim dan kualiti buah) apabila diaplikasikan pada dos yang berbeza (0, 15, 30, 45 dan 60 mg L-1). Bahan kimia diaplikasikan selepas pindah tanam anak-anak benih. Sejumlah 12 plot tanaman termasuk 3 tumbuhan untuk setiap dos telah digunakan dalam reka bentuk uji kaji plot rawak. Walaupun terdapat kesan positif pada kandungan prolina daun dan aktiviti enzim, dos Pro-Ca 45 dan 60 mg L-1 menunjukkan kesan negatif pada parameter morfologi (tidak termasuk diameter batang), hasil, pigmen daun (tidak termasuk klorofila) dan kandungan nutrien berbanding dengan parameter terkawal. Dos
Pro-Ca yang tinggi secara umumnya memberi kesan yang positif kepada kualiti buah manakala Pro-Ca dengan 15 mg L-1 tidak memberi kesan kepada parameter yang lain, kecuali penurunan hasil buah. Dos Pro-Ca 30 mg L-1 menunjukkan dos yang paling positif pada parameter yang dikaji. Pembangunan vegetatif, hasil, pengambilan nutrien dan kandungan prolina yang tertinggi telah diperoleh daripada tumbuhan yang dikenakan dos Pro- Ca 30 mg L-1. Malah, dos tersebut meningkatkan kepejalan buah, TSS, nilai EC dan aktiviti enzim jika dibandingkan dengan parameter terkawal. Penyimpanan timun selepas penuaian merupakan kriteria yang amat penting, kadar penurunan berat sebanyak 29.4% adalah lebih rendah pada buah yang diperoleh daripada tumbuhan yang menerima dos Pro-Ca 30 mg L-1 jika dibandingkan dengan tumbuhan yang terkawal. Oleh itu, aplikasi Pro-Ca dengan dos 30 mg
L-1, selain kesan positif terhadap pertumbuhan tumbuhan, hasil dan kualiti buah, ia juga menunjukkan peningkatan hayat simpan buah buahan dalam penuaian timun dalam rumah hijau.
Kata kunci: Hasil; kalsium proheksadion; kualiti buah; pertumbuhan; timun
RUJUKAN
Aebi, H. 1984. Catalase in vitro. Methods in Enzymology 105: 121-126.
Aghdam,
M.S. 2013. Mitigation of postharvest chilling injury in tomato fruit by
prohexadione calcium. Journal of
Agriculture and Food Technology 50(5): 1029-1033.
Akdemir,
S. 2018. Effects of paclobutrazol and
prohexadione-calcıum applications on lettuce (Lactuca sativa L.) seedlings quality and plant
growth. Ahi
Evran University. MSc Thesis (Unpublished).
Altuntaş, Ö. 2016. The effects of prohexadione-Ca threatments on plant growth,
nutrient elements uptake and fruit quality in tomato. Yuzuncu Yıl University Journal of Agricultural Sciences 26(1): 98-105.
Altıntaş,
S. 2011. Effects of prohexadione‑calcium with three rates of phosphorus
and chlormequat chloride on vegetative and generative growth of tomato. African Journal of Biotechnology 75:
17142-17151.
Arnon, D.I.
1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24(1): 1-15.
Asin, L., Alegre, S. & Montserrat, R. 2007. Effect of paclobutrazol, prohexadione-Ca, deficit
irrigation, summer pruning and root pruning on shoot growth, yield, and return
bloom, in a ‘Blanquilla’ pear orchard. Scientia
Horticulturae 113(2): 142-148.
BASF. 2019. Agricultural
Solutions. https://www.agro.basf.com.tr/tr/Ürünler/Ürün
Bilgileri/Velonta®-TR.html. Accessed on 10 Febuary 2018.
Bates, L.S., Waldren, R.P.
& Teare, I.D. 1973. Rapid determination of free proline for water stress
studies. Plant and Soil 39(1): 205-207.
Beauchamp, C. &
Fridovich, I. 1971. Superoxide dismutase:
Improved assay and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1): 276-287.
Bekheta, M.A., Abdelhamid, M.T.
& El-Morsi, A.A. 2009. Physiological response of Vicia faba to prohexadione-calcium under saline conditions. Planta Daninha 27(4): 769-779.
Bizjak,
J., Jakopic, J., Slatnar, A., Stampar, F., Stich, K., Halbwirth, H., Zadravec,
P. & Veberic, R. 2012. Late prohexadione-calcium
application on maturing apple cv. ‘Braeburn’ fruit reduces anthocyanins and
alters the phenolic content. Europen
Journal of Horticultural Science 77(4): 154-162.
Borzouei, A., Kafi, M.,
Akbari-Ghogdi, E. & Mousavi-Shalmani, M. 2012. Long term salinity stress in
relation to lipid peroxidation, super oxide dismutase activity and proline
content of salt sensitive and salt-tolerant wheat cultivars. Chilean Journal of Agricultural Research 72(4): 476-482.
Bremner, J.M. 1965. Total
nitrogen. In Methods of Soil Analysis, part 2, edited by Black, C.A.
Madison, Wisconsin: American Society of Agronomy. pp. 1149-1178.
Brown, A. 2007. Understanding Food Principles and
Preparation. Boston: Cengage Learning.
Chettri, M.K., Cook, C.M.,
Vardaka, E., Sawidis, T. & Lanaras, T. 1998. The effect of Cu, Zn and Pb on
the chlorophyll content of the lichens Cladonia convoluta and Cladonia
rangiformis. Environmental and
Experimental Botany 39(1):
1-10.
Demir,
K. & Başak, H. 2008. Applications that provide growth control in
vegetable seedlings. Seed Congress, Cappadocia. pp. 207-210.
De Saint
Germain, A., Ligerot, Y., Dun, E.A., Pillot, J.P., Ross, J.J., Beveridge, C.A.
& Rameau, C. 2013. Strigolactones stimulate internode elongation
independently of gibberellins. Plant
Physiology 163(2): 1012-1025.
Dragisic-Maksimovic,
J.J., Poledica, M.M., Radivojevi, D.D. & Milivojevic, J.M. 2017. Enzymatic
profile of ′Willamette′ raspberry leaf and fruit affected by
prohexadione-Ca and young canes removal treatments. Journal of Agricultural and Food Chemistry 65(24):
5034-5040.
Edge, R. & Truscott, T.G. 2018. Singlet oxygen and free radical
reactions of retinoids and carotenoids - A review. Antioxidants 7(1): 5.
El-Ghorab,
A.H., El-Massry, K., Alsohaimi, I.H., Hamza, M., Shaheen, M.S., Bekheta, M.A.,
Anees, A.K., Imran, M. & El-sherif, M.A. 2019. The impact of
some bioregulators on growth, chemical characters and radical scavenging
properties of onion volatile oil. Pakistan Journal Agricultural Science 56(4): 828-838.
Fard,
J.R., Kafi, M. & Naderi, R. 2015. The enhancement of drought stress
tolerance of kentucky bluegrass by prohexadione-calcium treatment. Journal of Ornamental Plants 5(4): 197-204.
Freitas, S.T., Jiang, C.Z. & Mitcham, E.J. 2012. Mechanisms involved in calcium deficiency development in
tomato fruit in response to gibberellins. Journal
of Plant Growth Regulation 31(2): 221-234.
Ghiasi,
N. & Razavi, F. 2013. Impact of postharvest prohexadione calcium treatment
on PAL activity in tomato fruit in response to chilling stress. Iranian Journal of Plant Physiology 4(1): 865-871.
Gül, A., Tüzel, İ.H.,
Okur, B., Tuncay, Ö., Aykut, N. & Engindeniz, S. 2000. Cucumber
Cultivation with Soilless Agriculture Technique in Greenhouse. Scientific
and Technological Research Institution of Turkey (TUBITAK).
Kacar,
B. & İnal, A. 2008. Plant analysis. Nobel Pres. 1241: 891.
Kang, S.M., Kim, J.T.,
Hamayun, M., Hwang, I.C., Khan, A.L., Kim, Y.H., Lee, J.H. & Lee, I.J.
2010. Influence of prohexadione-calcium on growth and gibberellins content of
Chinese cabbage grown in alpine region of South Korea. Scientia Horticulturae 125(2): 88-92.
Kaptan, M.A. & Aydın, M. 2007. Effects of different
nitrogen doses on yield and some quality parameters of cucumber cultivation in
soilless culture. Journal of Adnan Menderes Universıty Agricultural
Faculty 4(1-2): 77-81.
Kim, H.M., Lee, H.R., Kang, J.H. & Hwang, S.J. 2019. Prohexadione-calcium
application during vegetative growth affects growth of mother plants, runners,
and runner plants of Maehyang strawberry. Agronomy 9(3): 155.
Kim,
H.Y., Lee, I.J., Hamayun, M., Kim, J.T., Won, J.G., Hwang, I.C. & Kim, K.U.
2007. Effect of prohexadione-calcium on growth components and endogenous
gibberellins contents of rice (Oryza
sativa L.). Journal of Agronomy and
Crop Science 193(6): 445-451.
Kim, Y.H., Khan, A.L.,
Hamayun, M., Kim, J.T., Lee, J.H., Hwang, I.C., Yoon, C.S. & Lee, I.J.
2010. Effects of prohexadione calcium on growth and gibberellins contents of Chrysanthemum morifolium R. cv Monalisa
White. Scientia Horticulturae 123(3):
423-427.
Lichtenthaler, H.K. &
Wellburn, A.R. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transctions 11(5): 591-592.
Omran, R.G. 1980. Peroxide levels and the
activities of catalase, peroxidase, and indoleacetic acid oxidase during and
after chilling cucumber seedlings. Plant
Physiology 65(2): 407-408.
Özbay, N. & Süslüoğlu, Z. 2016. Assessment of growth
regulator prohexadione calcium as priming agent for germination enhancement of
pepper at low temperature. The Journal of
Animal and Plant Sciences 26: 1652-1658.
Özbay, N. & Ergun, N. 2015. Prohexadione calcium on the growth
and quality of eggplant seedlings. Pesquisa Agropecuária Brasileira 50(10): 932-938.
Poledica,
M.M., Milivojevic, J.M., Radivojevic, D.D. & Maksimovic, J.J.D. 2012.
Prohexadione-Ca and young cane removal treatments control growth, productivity,
and fruit quality of the Willamette raspberry. Turkish Journal Agriculture and Forestry 36(6): 680-687.
Rademacher, W., Saarloos,
K., Garuz-Porte, J.A., Riera Forcades, F., Senechal, Y., Andreotti, C.,
Sinelli, F., Sabatini, E. & Costa, G. 2004. Impact of prohexadione–Ca on
the vegetative and reproductive performance of apple and pear trees. European Journal of Horticultural Science 69(6): 221-228.
Spinelli,
F., Speakman, J.B., Rademacher, W., Halbwirth, H., Stich, K. & Costa, G.
2005. Luteoforol, a flavan 4-ol, is induced in pome fruits by prohexadione-Ca
and shows phytoalexin-like properties against Erwinia amylovora and other plant pathogens. Europen Journal of Plant Pathology 112(2): 133-142.
Ugur,
A. & Kavak, S. 2007. The effects of PP 333 and CCC on seed germination and
seedling height control of tomato. Acta
Horticulturae 729: 205-208.
Zhang,
Y.P., Jia, F.F., Zhang, X.M., Qiao, Y.X., Shi, K., Zhou, Y.H. & Yu, J.Q.
2012. Temperature effects on the reactive oxygen species formation and
antioxidant defence in roots of two cucurbit species with contrasting root zone
temperature optima. Acta Physiologiae
Plantarum 34(2): 713-720.
*Pengarang untuk surat-menyurat;
email: hbasak@ahievran.edu.tr
|