Sains Malaysiana 51(9)(2022): 3069-3079

http://doi.org/10.17576/jsm-2022-5109-26

Perbandingan Penghasilan Siderofor daripada Bacillus aryabhattai, Bacillus megaterium dan Bacillus cereus

(Comparison of Siderophore Production by Bacillus aryabhattai, Bacillus megaterium, and Bacillus cereus)

LEE SHIN YIN¹, YONG SHIH NEE², KUAN SENG HOW^2,3 & SYLVIA CHIENG^1,*  

¹Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

²Department of Mechanical and Material Engineering, Lee Kong Chian Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Sg Long Campus, 43000 Kajang, Selangor Darul Ehsan, Malaysia

³Centre for Photonics and Advanced Materials Research, Universiti Tunku Abdul Rahman, Sg Long Campus, 43000 Kajang, Selangor Darul Ehsan, Malaysia

Diserahkan: 14 Mac 2022/Diterima: 10 Mei 2022

Abstrak

Besi merupakan unsur yang penting bagi proses metabolisme organisma seperti kitar asid trikarboksilik, pengangkutan elektron dan fosforilasi oksidatif. Walau bagaimanapun, sumber besi di persekitaran luar sentiasa berada dalam bentuk kompleks yang tidak tersedia untuk pengambilan terus. Bagi mengatasi pengehadan besi, mikroorganisma berupaya untuk menghasilkan siderofor, sejenis pengkelat Fe(III) yang berafinan tinggi. Salah satu genus bakteria yang dapat menghasilkan siderofor adalah Bacillus. Dalam kajian ini, penghasilan dan keupayaan pengkompleksan siderofor Bacillus cereus, Bacillus megaterium dan Bacillus aryabhattai ditentukan melalui asai piring agar dan cecair krom azurol S (CAS). Kesan sumber karbon (glukosa, maltosa dan gliserol) ke atas penghasilan siderofor oleh Bacillus turut dikaji. Hasil kajian telah menunjukkan bahawa B. cereus yang dikulturkan dalam kaldu LB berupaya untuk menghasilkan siderofor yang tertinggi berbanding B. aryabhattai dan B. megaterium. Siderofor yang dihasilkan oleh ketiga-tiga spesies berupaya untuk berkompleks dengan Mn(II), Zn(II) dan Cu(II). Hasil kajian turut menunjukkan pengkulturan dengan maltosa telah memberikan penghasilan siderofor yang paling tinggi bagi B. aryabhattai dan B. megaterium.

Kata kunci: Bacillus aryabhattai; Bacillus cereus; Bacillus megaterium; siderofor

Abstract

Iron is an essential element for metabolic processes of organisms such as the tricarboxylic acid cycle, electron transport, and oxidative phophorylation. However, iron resources in the environment are always in complex forms that are not readily available for direct intake. To overcome iron limitation, microorganisms are able to produce a high affinity Fe(III) chelator, known as siderophore. One of the bacterial genus that can produced siderophore is Bacillus. In this study, the production and complexing ability of siderophore from Bacillus cereus, Bacillus megaterium, and Bacillus aryabhattai were determined through chrome azurol S (CAS) agar plate and liquid assay. The effect of carbon sources (glucose, maltose and glycerol) on siderophore production by the bacteria were also studied. The results demonstrated the ability of B. cereus to produce the most siderophore in LB broth in comparison to B. aryabhattai and B. megaterium. Siderophores produced by all three species were capable of complexing with Mn(II), Zn(II) and Cu(II). The results also showed that production of siderophore was the most effective with maltose as carbon source for B. aryabhattai and B. megaterium.

Keywords: Bacillus aryabhattai; Bacillus cereus; Bacillus megaterium; siderophore

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^*Pengarang untuk surat-menyurat; email: sylvia@ukm.edu.my